Experiment set3IT023 for Bacteroides thetaiotaomicron VPI-5482

(+)-Arabinogalactan carbon source

Group: carbon source
Media: Varel_Bryant_medium + (+)-Arabinogalactan (5 mg/ml)
Culturing: Btheta_ML6, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 17-Aug-17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 13 genes in this experiment

For carbon source (+)-Arabinogalactan in Bacteroides thetaiotaomicron VPI-5482

For carbon source (+)-Arabinogalactan across organisms

SEED Subsystems

Subsystem	#Specific
Predicted carbohydrate hydrolases	1
Xylose utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Starch and sucrose metabolism
• Nucleotide sugars metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
(1,4)-β-D-xylan degradation	2	1	1