Experiment set3IT019 for Pseudomonas syringae pv. syringae B728a

KB with Mitomycin C 0.625 ug/ml

Group: stress
Media: KB + Mitomycin C (0.625 ug/ml)
Culturing: SyringaeB728a_ML2, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on 2/15/18
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate

Specific Phenotypes

For 55 genes in this experiment

For stress Mitomycin C in Pseudomonas syringae pv. syringae B728a

For stress Mitomycin C across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, UvrABC system	2
Ammonia assimilation	1
DNA-replication	1
DNA repair, bacterial	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
HMG CoA Synthesis	1
Leucine Degradation and HMG-CoA Metabolism	1
Peptidoglycan Biosynthesis	1
Ribosome biogenesis bacterial	1
Serine-glyoxylate cycle	1
Transport of Zinc	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Pyrimidine metabolism
• Glutamate metabolism
• Methionine metabolism
• Valine, leucine and isoleucine degradation
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis
• Nitrogen metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate biosynthesis I	2	2	2
L-glutamine degradation I	1	1	1
L-glutamine degradation II	1	1	1
ammonia assimilation cycle III	3	3	2
pseudouridine degradation	2	1	1
L-glutamate and L-glutamine biosynthesis	7	5	2
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	3	1
L-leucine degradation I	6	5	1
L-citrulline biosynthesis	8	7	1
superpathway of L-citrulline metabolism	12	9	1