Experiment set3IT018 for Pseudomonas fluorescens FW300-N1B4

LB with Rifampicin 0.004 mg/ml

Group: stress
Media: LB + Rifampicin (0.004 mg/ml)
Culturing: pseudo1_N1B4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 26 (C), shaken=orbital
By: Adam on 12/15/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1036 F5,F6

Specific Phenotypes

For 10 genes in this experiment

For stress Rifampicin in Pseudomonas fluorescens FW300-N1B4

For stress Rifampicin across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	1
Experimental tye	1
Glutamate dehydrogenases	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nitrogen metabolism
• Glutamate metabolism
• Arginine and proline metabolism
• Histidine metabolism
• Sulfur metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate degradation I	1	1	1
assimilatory sulfate reduction III	3	3	1
L-alanine degradation II (to D-lactate)	3	3	1
ethene biosynthesis IV (engineered)	3	2	1
assimilatory sulfate reduction I	4	4	1
L-histidine degradation I	4	4	1
tRNA processing	10	10	2
L-histidine degradation II	5	5	1
L-histidine degradation III	6	4	1
L-glutamate degradation XI (reductive Stickland reaction)	7	3	1
4-aminobutanoate degradation V	7	2	1
L-histidine degradation VI	8	7	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	1
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)	10	8	1
L-glutamate degradation V (via hydroxyglutarate)	10	6	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	12	1
methylaspartate cycle	19	9	1