Experiment set3IT018 for Caulobacter crescentus NA1000

PYE with Cobalt chloride hexahydrate 0.08 mM

Group: stress
Media: PYE + Cobalt chloride hexahydrate (0.08 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.0 generations
By: Adam on 1-May-17
Media components: 2 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.8 mM Magnesium sulfate, 0.5 mM Calcium chloride
Growth plate: 1706 A5

Specific Phenotypes

For 5 genes in this experiment

For stress Cobalt chloride hexahydrate in Caulobacter crescentus NA1000

For stress Cobalt chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Cobalamin synthesis	1
Coenzyme B12 biosynthesis	1
Hemin transport system	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Riboflavin metabolism
• Porphyrin and chlorophyll metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
benzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
adeninyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
5-hydroxybenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
5-methoxybenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
5-methoxy-6-methylbenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
adenosylcobalamin biosynthesis from adenosylcobinamide-GDP I	3	1	1
2-methyladeninyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
5-methylbenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
superpathway of adenosylcobalamin salvage from cobinamide I	8	4	1
superpathway of adenosylcobalamin salvage from cobinamide II	9	4	1
adenosylcobalamin biosynthesis II (aerobic)	33	15	1
adenosylcobalamin biosynthesis I (anaerobic)	36	14	1