Experiment set3IT017 for Pseudomonas fluorescens FW300-N1B4

Compare to:

LB with Spectinomycin dihydrochloride pentahydrate 0.05 mg/ml

200 most important genes:

  gene name fitness t score description  
Pf1N1B4_327 -3.9 -2.7 hypothetical protein compare
Pf1N1B4_769 -3.9 -4.6 Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3) compare
Pf1N1B4_1 -3.6 -3.4 Colicin V production protein compare
Pf1N1B4_3362 -3.1 -4.7 Multidrug resistance protein B compare
Pf1N1B4_4772 -3.1 -5.2 RND efflux system, inner membrane transporter CmeB conserved
Pf1N1B4_1399 -3.0 -2.9 FIG003573: hypothetical protein compare
Pf1N1B4_3093 -3.0 -2.7 Outer membrane protein H precursor compare
Pf1N1B4_652 -2.9 -10.8 Sensory box histidine kinase compare
Pf1N1B4_1307 -2.8 -2.7 3-dehydroquinate dehydratase II (EC 4.2.1.10) compare
Pf1N1B4_2609 -2.7 -13.2 Catalase (EC 1.11.1.6) compare
Pf1N1B4_289 -2.5 -16.8 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54) compare
Pf1N1B4_2004 -2.5 -2.9 putative membrane protein compare
Pf1N1B4_2114 -2.5 -7.0 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf1N1B4_5690 -2.5 -5.1 Putative TEGT family carrier/transport protein compare
Pf1N1B4_2949 -2.4 -2.3 FIG00956396: hypothetical protein compare
Pf1N1B4_2106 -2.4 -3.9 Hydrogen peroxide-inducible genes activator compare
Pf1N1B4_58 -2.3 -8.1 Transaldolase (EC 2.2.1.2) compare
Pf1N1B4_1462 -2.3 -13.6 Oligosaccharide repeat unit polymerase Wzy; O-antigen ligase compare
Pf1N1B4_5991 -2.3 -2.8 Transcriptional regulator, AraC family compare
Pf1N1B4_959 -2.3 -13.4 Stringent starvation protein A compare
Pf1N1B4_3246 -2.2 -4.8 RND efflux system, inner membrane transporter CmeB compare
Pf1N1B4_5383 -2.2 -7.6 hypothetical protein compare
Pf1N1B4_1315 -2.2 -7.9 IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) compare
Pf1N1B4_3445 -2.1 -3.6 Succinylarginine dihydrolase (EC 3.5.3.23) compare
Pf1N1B4_4611 -2.1 -3.4 GlpG protein (membrane protein of glp regulon) compare
Pf1N1B4_3833 -2.1 -9.3 Major porin and structural outer membrane porin OprF compare
Pf1N1B4_2037 -2.1 -3.6 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf1N1B4_2917 -2.0 -8.9 FIG140336: TPR domain protein compare
Pf1N1B4_888 -2.0 -4.3 Transglycosylase, Slt family compare
Pf1N1B4_2208 -2.0 -6.2 Polyphosphate kinase (EC 2.7.4.1) compare
Pf1N1B4_4636 -1.9 -2.4 TPR domain protein in aerotolerance operon compare
Pf1N1B4_1296 -1.9 -10.5 Biosynthetic arginine decarboxylase (EC 4.1.1.19) (from data) compare
Pf1N1B4_3091 -1.9 -4.5 Membrane-associated zinc metalloprotease compare
Pf1N1B4_1441 -1.9 -10.4 Putative esterase, FIGfam005057 compare
Pf1N1B4_1619 -1.8 -9.8 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf1N1B4_2739 -1.8 -1.5 acetyltransferase, GNAT family compare
Pf1N1B4_1403 -1.8 -7.2 3'-to-5' exoribonuclease RNase R compare
Pf1N1B4_1375 -1.7 -8.2 Mannose-6-phosphate isomerase (EC 5.3.1.8) conserved
Pf1N1B4_1906 -1.7 -4.3 Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25) compare
Pf1N1B4_5838 -1.7 -3.0 FIG00956589: hypothetical protein compare
Pf1N1B4_814 -1.7 -2.4 Transcriptional regulator, PadR family compare
Pf1N1B4_3812 -1.7 -8.3 Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10) compare
Pf1N1B4_2354 -1.6 -4.7 twitching motility protein PilH compare
Pf1N1B4_2401 -1.6 -1.2 Lipoprotein, putative compare
Pf1N1B4_578 -1.6 -3.2 enoyl-CoA hydratase, R-specific compare
Pf1N1B4_710 -1.6 -3.5 Transporter, LysE family compare
Pf1N1B4_2105 -1.6 -2.5 ATP-dependent DNA helicase RecG (EC 3.6.1.-) compare
Pf1N1B4_932 -1.5 -2.1 FIG00963370: hypothetical protein compare
Pf1N1B4_2314 -1.5 -2.4 FIG028932: hypothetical protein compare
Pf1N1B4_3081 -1.5 -12.8 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf1N1B4_5010 -1.5 -5.4 Alkyl hydroperoxide reductase protein F (EC 1.6.4.-) compare
Pf1N1B4_4640 -1.5 -4.8 MoxR-like ATPase in aerotolerance operon compare
Pf1N1B4_4531 -1.5 -2.4 FIG01221261: hypothetical protein compare
Pf1N1B4_4699 -1.5 -1.5 Multidrug translocase MdfA compare
Pf1N1B4_3866 -1.5 -2.3 Adenine phosphoribosyltransferase (EC 2.4.2.7) compare
Pf1N1B4_5668 -1.5 -2.8 Peptidase, U7 family compare
Pf1N1B4_835 -1.5 -2.8 Trehalose-6-phosphate hydrolase (EC 3.2.1.93) compare
Pf1N1B4_1544 -1.5 -7.0 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf1N1B4_1105 -1.5 -2.9 4-carboxymuconolactone decarboxylase (EC 4.1.1.44) compare
Pf1N1B4_2283 -1.5 -2.0 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf1N1B4_4051 -1.5 -3.2 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42) compare
Pf1N1B4_1614 -1.4 -2.9 Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state) compare
Pf1N1B4_1153 -1.4 -2.8 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf1N1B4_683 -1.4 -2.6 Fumarate hydratase class I, aerobic (EC 4.2.1.2) compare
Pf1N1B4_5927 -1.4 -11.9 Capsular polysaccharide biosynthesis protein compare
Pf1N1B4_1683 -1.4 -1.6 Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17) compare
Pf1N1B4_4700 -1.4 -2.4 FIG00956898: hypothetical protein compare
Pf1N1B4_3730 -1.4 -3.4 Sodium-dependent transporter compare
Pf1N1B4_2489 -1.4 -5.5 Biotin synthesis protein BioH compare
Pf1N1B4_2377 -1.4 -5.8 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf1N1B4_3454 -1.4 -4.8 Mg/Co/Ni transporter MgtE / CBS domain compare
Pf1N1B4_5370 -1.4 -1.6 hypothetical protein compare
Pf1N1B4_1624 -1.3 -6.0 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf1N1B4_1844 -1.3 -8.3 Oligopeptidase A (EC 3.4.24.70) compare
Pf1N1B4_2959 -1.3 -4.7 FIG067310: hypothetical protein compare
Pf1N1B4_1626 -1.3 -6.0 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf1N1B4_2210 -1.3 -2.6 Glutamate transport ATP-binding protein compare
Pf1N1B4_2791 -1.3 -2.2 Putative inner membrane protein (Fragment) compare
Pf1N1B4_4771 -1.3 -2.3 Probable transmembrane protein compare
Pf1N1B4_1703 -1.3 -3.0 Methionine ABC transporter permease protein compare
Pf1N1B4_1234 -1.3 -3.9 Exodeoxyribonuclease V alpha chain (EC 3.1.11.5) compare
Pf1N1B4_4731 -1.3 -2.9 Sigma-54 dependent transcriptional regulator compare
Pf1N1B4_5829 -1.3 -2.2 FIG005107: hypothetical protein compare
Pf1N1B4_197 -1.3 -2.1 FIG139991: Putative thiamine pyrophosphate-requiring enzyme compare
Pf1N1B4_4024 -1.3 -2.5 Tryptophan synthase beta chain like (EC 4.2.1.20) compare
Pf1N1B4_3751 -1.3 -1.7 FIG00953060: hypothetical protein compare
Pf1N1B4_539 -1.3 -1.9 Transcriptional regulators compare
Pf1N1B4_5972 -1.3 -2.8 Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1) compare
Pf1N1B4_799 -1.3 -2.1 hypothetical protein compare
Pf1N1B4_3368 -1.3 -2.1 Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54) compare
Pf1N1B4_3525 -1.3 -2.1 hydrolase, alpha/beta fold family compare
Pf1N1B4_2006 -1.3 -2.6 hypothetical protein compare
Pf1N1B4_875 -1.3 -2.7 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf1N1B4_3074 -1.3 -1.6 HesA/MoeB/ThiF family protein related to EC-YgdL compare
Pf1N1B4_1830 -1.2 -6.2 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf1N1B4_350 -1.2 -4.2 Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51) compare
Pf1N1B4_1827 -1.2 -3.5 FIG00953482: hypothetical protein compare
Pf1N1B4_2353 -1.2 -2.9 twitching motility protein PilG compare
Pf1N1B4_958 -1.2 -2.5 Stringent starvation protein B compare
Pf1N1B4_1469 -1.2 -9.0 Heptose kinase WapQ, eukaryotic type compare
Pf1N1B4_1617 -1.2 -5.9 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1) compare
Pf1N1B4_236 -1.2 -3.0 Molybdenum cofactor biosynthesis protein MoaB compare
Pf1N1B4_5891 -1.2 -3.0 FIG00961753: hypothetical protein compare
Pf1N1B4_5284 -1.2 -2.6 hypothetical protein compare
Pf1N1B4_1610 -1.2 -3.7 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf1N1B4_3482 -1.2 -6.1 outer membrane protein H1 compare
Pf1N1B4_4028 -1.2 -3.4 NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3) compare
Pf1N1B4_2026 -1.2 -2.9 Transcriptional regulator compare
Pf1N1B4_3447 -1.2 -4.0 Succinylglutamate desuccinylase (EC 3.5.1.96) compare
Pf1N1B4_630 -1.2 -3.7 TPR repeat containing exported protein; Putative periplasmic protein contains a protein prenylyltransferase domain compare
Pf1N1B4_3889 -1.1 -1.4 hypothetical protein compare
Pf1N1B4_763 -1.1 -3.4 Ferric iron ABC transporter, ATP-binding protein compare
Pf1N1B4_3143 -1.1 -2.9 RecA protein compare
Pf1N1B4_3559 -1.1 -7.6 Aromatic amino acid transport protein AroP compare
Pf1N1B4_2493 -1.1 -2.2 Acyl-CoA dehydrogenase (EC 1.3.8.7) compare
Pf1N1B4_4063 -1.1 -3.1 CrcB protein compare
Pf1N1B4_275 -1.1 -2.0 Organic hydroperoxide resistance transcriptional regulator compare
Pf1N1B4_3997 -1.1 -3.6 Uncharacterized protein YtfM precursor compare
Pf1N1B4_4107 -1.1 -1.7 Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog compare
Pf1N1B4_3501 -1.1 -4.8 BarA sensory histidine kinase (= VarS = GacS) compare
Pf1N1B4_348 -1.1 -3.0 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf1N1B4_617 -1.1 -1.7 Carbamate kinase (EC 2.7.2.2) compare
Pf1N1B4_5884 -1.1 -1.6 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf1N1B4_1749 -1.1 -1.6 Prevent host death protein, Phd antitoxin compare
Pf1N1B4_3508 -1.1 -3.7 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf1N1B4_3364 -1.1 -2.0 Cyn operon transcriptional activator compare
Pf1N1B4_675 -1.1 -2.5 hypothetical protein compare
Pf1N1B4_1336 -1.1 -1.5 Putative multicopper oxidases compare
Pf1N1B4_2888 -1.1 -3.5 FIG00961017: hypothetical protein compare
Pf1N1B4_1627 -1.1 -3.0 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf1N1B4_2075 -1.1 -2.7 ABC-type antimicrobial peptide transport system, permease component compare
Pf1N1B4_493 -1.1 -2.5 hypothetical protein compare
Pf1N1B4_1271 -1.1 -4.5 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) compare
Pf1N1B4_1831 -1.1 -6.8 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf1N1B4_4318 -1.1 -2.6 hypothetical protein compare
Pf1N1B4_2866 -1.0 -2.7 Periplasmic hemin-binding protein compare
Pf1N1B4_3329 -1.0 -4.8 Putative permease PerM (= YfgO) compare
Pf1N1B4_5684 -1.0 -2.8 Glyceraldehyde-3-phosphate dehydrogenase, putative compare
Pf1N1B4_239 -1.0 -2.8 Similar to ribosomal large subunit pseudouridine synthase A compare
Pf1N1B4_2682 -1.0 -1.7 Antiholin-like protein LrgA compare
Pf1N1B4_3038 -1.0 -4.5 Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61) compare
Pf1N1B4_4518 -1.0 -2.5 Possible pyrimidine permease in reductive pathway compare
Pf1N1B4_4729 -1.0 -2.0 Hemolysin-type calcium-binding region compare
Pf1N1B4_1236 -1.0 -4.4 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf1N1B4_4472 -1.0 -2.2 Transcriptional regulator, ArsR family compare
Pf1N1B4_4145 -1.0 -1.3 hypothetical protein compare
Pf1N1B4_5463 -1.0 -1.7 hypothetical protein compare
Pf1N1B4_2352 -1.0 -4.1 Glutathione synthetase (EC 6.3.2.3) compare
Pf1N1B4_1159 -1.0 -2.1 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf1N1B4_5187 -1.0 -0.7 5S RNA compare
Pf1N1B4_2872 -1.0 -5.9 FIG00955330: hypothetical protein compare
Pf1N1B4_2488 -1.0 -4.8 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf1N1B4_3636 -1.0 -1.2 FIG00953562: hypothetical protein compare
Pf1N1B4_644 -1.0 -1.6 Cold shock protein CspC compare
Pf1N1B4_233 -1.0 -2.7 Transcription regulator [contains diacylglycerol kinase catalytic domain] compare
Pf1N1B4_2491 -1.0 -2.7 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf1N1B4_2487 -1.0 -5.1 Biotin synthase (EC 2.8.1.6) compare
Pf1N1B4_2490 -0.9 -4.1 Biotin synthesis protein BioC compare
Pf1N1B4_2736 -0.9 -2.0 ATP-dependent RNA helicase PA3950 compare
Pf1N1B4_292 -0.9 -8.6 ErfK/YbiS/YcfS/YnhG family protein compare
Pf1N1B4_2795 -0.9 -2.5 Alkylphosphonate utilization operon protein PhnA compare
Pf1N1B4_4818 -0.9 -1.8 RNA polymerase sigma-70 factor, ECF subfamily compare
Pf1N1B4_1527 -0.9 -2.5 Queuosine Biosynthesis QueC ATPase compare
Pf1N1B4_5334 -0.9 -1.9 hypothetical protein compare
Pf1N1B4_1090 -0.9 -4.2 TldE protein, part of TldE/TldD proteolytic complex compare
Pf1N1B4_4368 -0.9 -2.7 Guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf1N1B4_3819 -0.9 -3.1 Methylisocitrate lyase (EC 4.1.3.30) compare
Pf1N1B4_1611 -0.9 -3.1 Nitrogen regulation protein NR(I) compare
Pf1N1B4_4256 -0.9 -1.7 Probable transmembrane protein compare
Pf1N1B4_5724 -0.9 -2.0 High-affinity iron permease compare
Pf1N1B4_2766 -0.9 -4.4 Catalase (EC 1.11.1.6) compare
Pf1N1B4_3789 -0.9 -2.2 Superfamily II DNA/RNA helicases, SNF2 family compare
Pf1N1B4_2712 -0.9 -3.2 tRNA-i(6)A37 methylthiotransferase compare
Pf1N1B4_373 -0.9 -2.1 5-methylaminomethyl-2-thiouridine-forming enzyme mnmC compare
Pf1N1B4_4585 -0.9 -2.0 Putative Isoquinoline 1-oxidoreductase subunit, Mll3835 protein compare
Pf1N1B4_967 -0.9 -1.7 hypothetical protein compare
Pf1N1B4_824 -0.9 -2.8 POSSIBLE MEMBRANE PROTEIN compare
Pf1N1B4_5745 -0.9 -1.2 FIG139928: Putative protease compare
Pf1N1B4_2483 -0.9 -2.5 DNA-binding domain of ModE / Molybdate-binding domain of ModE compare
Pf1N1B4_4482 -0.9 -2.3 FIG146518: Zn-dependent hydrolases, including glyoxylases compare
Pf1N1B4_1235 -0.9 -3.8 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf1N1B4_823 -0.9 -1.7 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf1N1B4_4666 -0.9 -2.2 Acriflavin resistance plasma membrane protein compare
Pf1N1B4_883 -0.9 -1.5 Hypothetical nudix hydrolase YeaB compare
Pf1N1B4_5446 -0.9 -2.0 hypothetical protein compare
Pf1N1B4_4921 -0.9 -2.2 Haloacid dehalogenase, type II compare
Pf1N1B4_5725 -0.9 -1.4 High-affinity iron permease compare
Pf1N1B4_2350 -0.8 -2.6 UPF0301 protein YqgE compare
Pf1N1B4_697 -0.8 -2.9 Glycine cleavage system transcriptional activator compare
Pf1N1B4_5430 -0.8 -2.0 hypothetical protein compare
Pf1N1B4_544 -0.8 -2.2 Small-conductance mechanosensitive channel compare
Pf1N1B4_3907 -0.8 -1.7 FIG00953287: hypothetical protein compare
Pf1N1B4_2975 -0.8 -1.5 Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12) compare
Pf1N1B4_3069 -0.8 -2.1 FIG00954614: hypothetical protein compare
Pf1N1B4_2168 -0.8 -4.2 FIG004064: hypothetical protein compare
Pf1N1B4_4491 -0.8 -2.7 ABC transporter permease protein compare
Pf1N1B4_2237 -0.8 -2.3 Ribonucleotide reductase, alpha subunit compare
Pf1N1B4_1704 -0.8 -2.2 hypothetical protein compare
Pf1N1B4_5464 -0.8 -1.6 Cointegrate resolution protein T compare
Pf1N1B4_1474 -0.8 -2.9 Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) compare


Specific Phenotypes

For 7 genes in this experiment

For stress Spectinomycin dihydrochloride pentahydrate in Pseudomonas fluorescens FW300-N1B4

For stress Spectinomycin dihydrochloride pentahydrate across organisms