Experiment set3IT017 for Pseudomonas fluorescens FW300-N1B4

Compare to:

LB with Spectinomycin dihydrochloride pentahydrate 0.05 mg/ml

200 most detrimental genes:

  gene name fitness t score description  
Pf1N1B4_1463 +4.5 29.7 hypothetical protein compare
Pf1N1B4_1461 +4.2 13.6 Protein fixF compare
Pf1N1B4_1464 +3.0 14.7 Glycosyl transferase compare
Pf1N1B4_973 +2.4 21.1 Cytochrome O ubiquinol oxidase subunit II (EC 1.10.3.-) compare
Pf1N1B4_969 +2.4 22.9 Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB compare
Pf1N1B4_972 +2.3 20.9 Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-) compare
Pf1N1B4_1466 +2.3 3.8 hypothetical protein compare
Pf1N1B4_262 +2.3 19.8 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf1N1B4_1465 +2.3 14.3 Carbamoyltransferase in large core OS assembly cluster compare
Pf1N1B4_970 +2.3 17.6 Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-) compare
Pf1N1B4_971 +2.2 18.2 Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-) compare
Pf1N1B4_1095 +1.9 14.7 Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-) compare
Pf1N1B4_2854 +1.8 7.0 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf1N1B4_1668 +1.8 10.3 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf1N1B4_3876 +1.7 10.3 Cytochrome c oxidase subunit CcoP (EC 1.9.3.1) compare
Pf1N1B4_336 +1.7 13.6 Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40) compare
Pf1N1B4_337 +1.6 8.6 O-antigen export system permease protein RfbD compare
Pf1N1B4_3878 +1.5 7.7 Cytochrome c oxidase subunit CcoO (EC 1.9.3.1) compare
Pf1N1B4_1460 +1.5 11.0 Lipid A core - O-antigen ligase and related enzymes compare
Pf1N1B4_2912 +1.5 2.9 GTP-binding and nucleic acid-binding protein YchF compare
Pf1N1B4_106 +1.5 5.8 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf1N1B4_1334 +1.4 2.6 FIG00955840: hypothetical protein compare
Pf1N1B4_3879 +1.4 11.3 Cytochrome c oxidase subunit CcoN (EC 1.9.3.1) compare
Pf1N1B4_3880 +1.4 10.3 hypothetical protein compare
Pf1N1B4_1417 +1.3 9.7 NAD(P)HX epimerase / NAD(P)HX dehydratase compare
Pf1N1B4_4205 +1.3 3.4 Probable signal peptide protein compare
Pf1N1B4_2853 +1.2 3.0 Pantoate--beta-alanine ligase (EC 6.3.2.1) compare
Pf1N1B4_1565 +1.2 4.2 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf1N1B4_340 +1.2 6.4 Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-) compare
Pf1N1B4_2858 +1.2 9.3 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf1N1B4_4969 +1.2 2.8 FIG00956189: hypothetical protein compare
Pf1N1B4_4717 +1.2 2.2 Agmatinase (EC 3.5.3.11) compare
Pf1N1B4_335 +1.2 8.0 Glycosyltransferase compare
Pf1N1B4_5924 +1.1 6.1 hypothetical protein compare
Pf1N1B4_1566 +1.1 6.5 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf1N1B4_2643 +1.1 1.9 Predicted zinc-binding protein compare
Pf1N1B4_338 +1.1 4.4 Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-) compare
Pf1N1B4_139 +1.1 3.0 Pyoverdin biosynthesis protein PvdN, putative aminotransferase, class V compare
Pf1N1B4_5136 +1.1 5.5 Carboxynorspermidine dehydrogenase compare
Pf1N1B4_919 +1.1 3.1 hypothetical protein compare
Pf1N1B4_2433 +1.1 3.6 FIG00953108: hypothetical protein compare
Pf1N1B4_1569 +1.1 8.8 Twin-arginine translocation protein TatC compare
Pf1N1B4_4436 +1.1 3.1 Glutaminase (EC 3.5.1.2) compare
Pf1N1B4_1418 +1.0 8.0 Epoxyqueuosine (oQ) reductase QueG compare
Pf1N1B4_753 +1.0 2.7 hypothetical protein compare
Pf1N1B4_4755 +1.0 3.3 ribosomal protein S6 glutaminyl transferase related protein compare
Pf1N1B4_3815 +1.0 4.6 FIG002781: Alpha-L-glutamate ligase family protein compare
Pf1N1B4_4280 +1.0 3.2 Myo-inositol 2-dehydrogenase (EC 1.1.1.18) compare
Pf1N1B4_1115 +1.0 7.6 Dna binding response regulator PrrA (RegA) compare
Pf1N1B4_5137 +1.0 4.7 Carboxynorspermidine decarboxylase, putative (EC 4.1.1.-) compare
Pf1N1B4_2859 +1.0 8.1 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
Pf1N1B4_647 +1.0 6.5 hypothetical protein compare
Pf1N1B4_3708 +1.0 2.6 Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family compare
Pf1N1B4_649 +1.0 3.1 Small-conductance mechanosensitive channel compare
Pf1N1B4_5448 +1.0 2.2 hypothetical protein compare
Pf1N1B4_4201 +1.0 2.4 Transcriptional regulator, LysR family compare
Pf1N1B4_1335 +1.0 4.0 Copper metallochaperone, bacterial analog of Cox17 protein compare
Pf1N1B4_4377 +1.0 2.1 Cell wall hydrolyses involved in spore germination compare
Pf1N1B4_4473 +1.0 2.0 Homoserine/homoserine lactone efflux protein compare
Pf1N1B4_572 +1.0 7.5 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf1N1B4_339 +0.9 1.6 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf1N1B4_74 +0.9 2.0 hypothetical protein compare
Pf1N1B4_3869 +0.9 7.1 Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein compare
Pf1N1B4_5674 +0.9 2.9 Strictosidine synthase precursor (EC 4.3.3.2) compare
Pf1N1B4_648 +0.9 8.5 Outer membrane porin, OprD family compare
Pf1N1B4_5353 +0.9 4.2 hypothetical protein compare
Pf1N1B4_2096 +0.9 2.1 Protein serine/threonine phosphatase PrpC, regulation of stationary phase compare
Pf1N1B4_1096 +0.9 4.1 Septum formation protein Maf compare
Pf1N1B4_103 +0.9 3.9 Histidine permease YuiF compare
Pf1N1B4_5658 +0.9 3.4 Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133) compare
Pf1N1B4_322 +0.9 1.7 nucleotide sugar epimerase/dehydratase WbpM compare
Pf1N1B4_1116 +0.9 7.0 Sensor histidine kinase PrrB (RegB) (EC 2.7.3.-) compare
Pf1N1B4_5649 +0.9 2.5 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34) compare
Pf1N1B4_5604 +0.9 2.7 Permeases of the major facilitator superfamily compare
Pf1N1B4_1888 +0.9 3.8 Glutamate-aspartate carrier protein compare
Pf1N1B4_1485 +0.8 2.9 FIG00963848: hypothetical protein compare
Pf1N1B4_2228 +0.8 3.2 FIG001590: Putative conserved exported protein precursor compare
Pf1N1B4_3872 +0.8 5.6 Putative analog of CcoH, COG3198 compare
Pf1N1B4_5239 +0.8 2.7 hypothetical protein compare
Pf1N1B4_4658 +0.8 2.6 hypothetical protein compare
Pf1N1B4_1094 +0.8 2.4 FIG005080: Possible exported protein compare
Pf1N1B4_929 +0.8 2.6 FIG00964214: hypothetical protein compare
Pf1N1B4_4523 +0.8 1.7 RNA polymerase sigma-70 factor, ECF subfamily compare
Pf1N1B4_3692 +0.8 1.8 Chaperone protein HtpG compare
Pf1N1B4_2284 +0.8 3.0 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf1N1B4_3241 +0.8 1.8 hypothetical protein compare
Pf1N1B4_6032 +0.8 1.8 Ribose operon repressor compare
Pf1N1B4_3817 +0.8 2.6 FIG008443: hypothetical protein compare
Pf1N1B4_2878 +0.8 5.8 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf1N1B4_4172 +0.8 2.3 hypothetical protein compare
Pf1N1B4_1411 +0.8 3.0 GTP-binding protein HflX compare
Pf1N1B4_2733 +0.8 1.8 DNA polymerase III alpha subunit (EC 2.7.7.7) compare
Pf1N1B4_627 +0.8 2.1 hypothetical protein compare
Pf1N1B4_1114 +0.8 4.0 ABC transporter, ATP-binding protein compare
Pf1N1B4_4103 +0.8 1.2 Flagellar synthesis regulator FleN compare
Pf1N1B4_2411 +0.8 2.2 FIG00953568: hypothetical protein compare
Pf1N1B4_5266 +0.8 1.8 hypothetical protein compare
Pf1N1B4_1691 +0.8 1.0 hypothetical protein compare
Pf1N1B4_2021 +0.8 1.2 hypothetical protein compare
Pf1N1B4_4043 +0.8 1.7 Type II and III secretion system family protein compare
Pf1N1B4_3625 +0.8 2.2 Flagellar synthesis regulator FleN compare
Pf1N1B4_4423 +0.8 2.5 ABC-type amino acid transport/signal transduction systems, periplasmic component/domain compare
Pf1N1B4_3871 +0.8 6.5 Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf1N1B4_2171 +0.8 1.9 Tyrosine recombinase XerC compare
Pf1N1B4_358 +0.8 4.8 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf1N1B4_2860 +0.8 3.7 FIG00954153: hypothetical protein compare
Pf1N1B4_3900 +0.8 2.8 Universal stress protein family COG0589 compare
Pf1N1B4_381 +0.8 1.5 hypothetical protein compare
Pf1N1B4_2339 +0.8 1.9 COG1872 compare
Pf1N1B4_5659 +0.8 6.0 NfuA Fe-S protein maturation compare
Pf1N1B4_5451 +0.8 1.9 hypothetical protein compare
Pf1N1B4_625 +0.7 3.0 FIG023103: Predicted transmembrane protein compare
Pf1N1B4_3870 +0.7 6.4 Type cbb3 cytochrome oxidase biogenesis protein CcoS, involved in heme b insertion compare
Pf1N1B4_2694 +0.7 5.6 Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-) compare
Pf1N1B4_3665 +0.7 2.4 Transcriptional regulator, TetR family compare
Pf1N1B4_4603 +0.7 1.2 hypothetical protein compare
Pf1N1B4_2048 +0.7 1.9 hypothetical protein compare
Pf1N1B4_2001 +0.7 2.4 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
Pf1N1B4_1866 +0.7 2.8 Methionine ABC transporter ATP-binding protein compare
Pf1N1B4_4975 +0.7 2.1 hypothetical protein compare
Pf1N1B4_4568 +0.7 1.3 FIG00793915: hypothetical protein compare
Pf1N1B4_3656 +0.7 0.8 Eukaryotic-type low-affinity urea transporter compare
Pf1N1B4_5574 +0.7 1.5 hypothetical protein compare
Pf1N1B4_2794 +0.7 1.8 FIG00953595: hypothetical protein compare
Pf1N1B4_2229 +0.7 5.9 Xaa-Pro aminopeptidase (EC 3.4.11.9) compare
Pf1N1B4_3816 +0.7 3.5 FIG139976: hypothetical protein compare
Pf1N1B4_4239 +0.7 3.0 hypothetical protein compare
Pf1N1B4_2303 +0.7 5.8 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf1N1B4_274 +0.7 3.2 Transcriptional regulator, LysR family compare
Pf1N1B4_5507 +0.7 1.3 hypothetical protein compare
Pf1N1B4_5644 +0.7 2.3 amino acid ABC transporter, permease protein compare
Pf1N1B4_5433 +0.7 2.4 hypothetical protein compare
Pf1N1B4_2047 +0.7 2.6 Chorismate--pyruvate lyase (EC 4.1.3.40) compare
Pf1N1B4_977 +0.7 2.4 Molybdenum cofactor biosynthesis protein MoaC compare
Pf1N1B4_614 +0.7 2.4 Arginine/ornithine antiporter ArcD compare
Pf1N1B4_3113 +0.7 5.0 Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77) compare
Pf1N1B4_846 +0.7 1.5 hypothetical protein compare
Pf1N1B4_5899 +0.7 1.6 FIG00954746: hypothetical protein compare
Pf1N1B4_731 +0.7 1.3 Scaffold protein for [4Fe-4S] cluster assembly ApbC, MRP-like compare
Pf1N1B4_1183 +0.7 5.4 Sodium-dependent transporter family protein compare
Pf1N1B4_4517 +0.7 1.7 Dihydropyrimidinase (EC 3.5.2.2) compare
Pf1N1B4_3025 +0.7 2.6 Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1) compare
Pf1N1B4_3435 +0.7 1.2 ABC transporter for L-Arginine and L-Citrulline, ATPase component (from data) compare
Pf1N1B4_2856 +0.7 2.8 Poly(A) polymerase (EC 2.7.7.19) compare
Pf1N1B4_4481 +0.7 1.6 Transcriptional regulator BkdR of isoleucine and valine catabolism operon compare
Pf1N1B4_259 +0.7 2.1 Glutathione peroxidase family protein compare
Pf1N1B4_5549 +0.7 1.8 hypothetical protein compare
Pf1N1B4_2701 +0.7 6.1 Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-) compare
Pf1N1B4_3487 +0.7 3.1 Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, aspartate compare
Pf1N1B4_733 +0.7 1.3 Methionyl-tRNA synthetase (EC 6.1.1.10) compare
Pf1N1B4_1146 +0.7 4.1 fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components (from data) compare
Pf1N1B4_1568 +0.7 2.1 Twin-arginine translocation protein TatB compare
Pf1N1B4_2485 +0.7 1.3 Competence protein F homolog, phosphoribosyltransferase domain; protein YhgH required for utilization of DNA as sole source of carbon and energy compare
Pf1N1B4_5806 +0.7 3.0 Aromatic hydrocarbon utilization transcriptional regulator CatR (LysR family) compare
Pf1N1B4_1135 +0.7 2.0 FIG00960055: hypothetical protein compare
Pf1N1B4_237 +0.7 2.2 Molybdopterin-guanine dinucleotide biosynthesis protein MobA compare
Pf1N1B4_3965 +0.7 1.3 FIG033889: YebC paralog in Betaproteobacteria compare
Pf1N1B4_5159 +0.7 2.5 Cellulose synthase operon protein C compare
Pf1N1B4_1246 +0.7 1.6 FIG00954047: hypothetical protein compare
Pf1N1B4_2718 +0.7 2.4 Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7) compare
Pf1N1B4_2852 +0.7 3.8 Aspartate 1-decarboxylase (EC 4.1.1.11) compare
Pf1N1B4_101 +0.7 1.7 hypothetical protein compare
Pf1N1B4_573 +0.7 4.4 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf1N1B4_1963 +0.7 2.8 O-antigen export system permease protein RfbD compare
Pf1N1B4_2442 +0.6 2.2 Electron transfer flavoprotein, beta subunit compare
Pf1N1B4_656 +0.6 4.4 Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1) compare
Pf1N1B4_909 +0.6 1.5 Ketosteroid isomerase-related protein compare
Pf1N1B4_5387 +0.6 2.9 hypothetical protein compare
Pf1N1B4_5833 +0.6 0.9 Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5) compare
Pf1N1B4_2331 +0.6 2.5 Thiazole biosynthesis protein ThiG compare
Pf1N1B4_149 +0.6 2.6 Pyoverdine sidechain non-ribosomal peptide synthetase PvdD compare
Pf1N1B4_4860 +0.6 1.2 Sensory box sensor histidine kinase/response regulator (EC 2.7.3.-) compare
Pf1N1B4_4806 +0.6 1.5 Argininosuccinate lyase (EC 4.3.2.1) compare
Pf1N1B4_4763 +0.6 2.5 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43) compare
Pf1N1B4_1561 +0.6 1.9 FIG00954300: hypothetical protein compare
Pf1N1B4_1514 +0.6 2.5 FIG022199: FAD-binding protein compare
Pf1N1B4_4402 +0.6 2.3 SoxH protein, homolog compare
Pf1N1B4_3719 +0.6 2.4 Glutathione peroxidase family protein compare
Pf1N1B4_4961 +0.6 2.0 FIG00441227: hypothetical protein compare
Pf1N1B4_2668 +0.6 4.4 DedA protein compare
Pf1N1B4_446 +0.6 2.0 Thymidylate kinase (EC 2.7.4.9) compare
Pf1N1B4_5591 +0.6 1.5 Vanillin dehydrogenase (Hydroxybenzaldehyde dehydrogenase) (EC 1.2.1.28) compare
Pf1N1B4_2382 +0.6 4.6 ATP-dependent RNA helicase RhlE compare
Pf1N1B4_4828 +0.6 1.5 hypothetical protein compare
Pf1N1B4_2071 +0.6 1.4 Protein phosphatase ImpM compare
Pf1N1B4_3399 +0.6 2.5 RNA polymerase sigma-70 factor, ECF subfamily compare
Pf1N1B4_4892 +0.6 1.4 Transcriptional regulator, AraC family compare
Pf1N1B4_1761 +0.6 2.9 Fibronectin type III domain protein compare
Pf1N1B4_5115 +0.6 2.0 sucrose ABC transporter, ATPase component (from data) compare
Pf1N1B4_974 +0.6 1.9 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf1N1B4_372 +0.6 1.7 UDP-galactose-lipid carrier transferase (EC 2.-.-.-) compare
Pf1N1B4_341 +0.6 2.0 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) compare
Pf1N1B4_429 +0.6 1.6 FIG00964214: hypothetical protein compare
Pf1N1B4_4336 +0.6 1.7 FMN-dependent NADH-azoreductase compare
Pf1N1B4_4526 +0.6 1.4 Myo-inositol 2-dehydrogenase (EC 1.1.1.18) compare
Pf1N1B4_1331 +0.6 1.6 Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-) compare
Pf1N1B4_4401 +0.6 2.4 Non-heme chloroperoxidase (EC 1.11.1.10) compare
Pf1N1B4_3961 +0.6 1.8 transcriptional regulator, MerR family compare
Pf1N1B4_3531 +0.6 1.8 FIG039061: hypothetical protein related to heme utilization compare
Pf1N1B4_2537 +0.6 1.7 DNA-binding response regulator, LuxR family, near polyamine transporter compare


Specific Phenotypes

For 7 genes in this experiment

For stress Spectinomycin dihydrochloride pentahydrate in Pseudomonas fluorescens FW300-N1B4

For stress Spectinomycin dihydrochloride pentahydrate across organisms