Experiment set3IT017 for Pseudomonas fluorescens FW300-N1B4

LB with Spectinomycin dihydrochloride pentahydrate 0.05 mg/ml

Group: stress
Media: LB + Spectinomycin dihydrochloride pentahydrate (0.05 mg/ml)
Culturing: pseudo1_N1B4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 26 (C), shaken=orbital
By: Adam on 12/15/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1036 C7,C8

Specific Phenotypes

For 7 genes in this experiment

For stress Spectinomycin dihydrochloride pentahydrate in Pseudomonas fluorescens FW300-N1B4

For stress Spectinomycin dihydrochloride pentahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Acid resistance mechanisms	1
Alginate metabolism	1
Arginine and Ornithine Degradation	1
Mannose Metabolism	1
Multidrug Resistance Efflux Pumps	1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	1
Polyamine Metabolism	1
Terminal cytochrome C oxidases	1
Thioredoxin-disulfide reductase	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Oxidative phosphorylation
• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism
• Selenoamino acid metabolism
• Nucleotide sugars metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
arginine dependent acid resistance	1	1	1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	2	1
putrescine biosynthesis I	2	2	1
D-mannose degradation I	2	1	1
arsenite to oxygen electron transfer	2	1	1
D-mannose degradation II	2	1	1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway)	3	3	1
putrescine biosynthesis II	3	3	1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
mannitol biosynthesis	3	2	1
arsenite to oxygen electron transfer (via azurin)	3	1	1
GDP-mannose biosynthesis	4	4	1
aerobic respiration II (cytochrome c) (yeast)	4	3	1
superpathway of putrescine biosynthesis	4	3	1
aerobic respiration I (cytochrome c)	4	3	1
mannitol degradation II	4	2	1
spermidine biosynthesis III	4	1	1
1,5-anhydrofructose degradation	5	2	1
Fe(II) oxidation	6	3	1
β-(1,4)-mannan degradation	7	2	1
superpathway of polyamine biosynthesis I	8	5	1
superpathway of polyamine biosynthesis II	8	5	1
colanic acid building blocks biosynthesis	11	9	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	9	1
superpathway of L-arginine and L-ornithine degradation	13	11	1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	7	1
superpathway of arginine and polyamine biosynthesis	17	14	1