Experiment set3IT015 for Caulobacter crescentus NA1000

PYE with Nickel (II) chloride hexahydrate 0.15 mM

Group: stress
Media: PYE + Nickel (II) chloride hexahydrate (0.15 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.8 generations
By: Adam on 1-May-17
Media components: 2 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.8 mM Magnesium sulfate, 0.5 mM Calcium chloride
Growth plate: 1706 A1

Specific Phenotypes

For 4 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Caulobacter crescentus NA1000

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Methionine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Methionine metabolism
• Cysteine metabolism
• Selenoamino acid metabolism
• Nitrogen metabolism
• Sulfur metabolism
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
hydrogen sulfide biosynthesis II (mammalian)	6	1	1