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Experiment set3IT010 for
Methanococcus maripaludis S2
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H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
200 most detrimental genes:
gene
name
fitness
t score
description
MMP
_RS08860
+2.8
16.0
MinD/ParA family protein
compare
MMP
_RS08855
+2.8
20.8
hypothetical protein
compare
MMP
_RS08675
+2.7
10.4
class III signal peptide-containing protein
compare
MMP
_RS00240
+2.6
23.2
type II secretion system F family protein
compare
MMP
_RS01290
+2.6
23.0
class III signal peptide-containing protein
compare
MMP
_RS01285
+2.5
22.5
hypothetical protein
compare
MMP
_RS00245
+2.5
23.4
type II secretion system F family protein
compare
MMP
_RS01295
+2.5
21.8
hypothetical protein
compare
MMP
_RS01280
+2.5
21.6
DUF515 domain-containing protein
compare
MMP
_RS01310
+2.5
22.9
hypothetical protein
compare
MMP
_RS01305
+2.5
22.7
hypothetical protein
compare
MMP
_RS00250
+2.5
21.4
Flp pilus assembly complex ATPase component TadA
compare
MMP
_RS01270
+2.4
21.5
prepilin peptidase
compare
MMP
_RS01315
+2.4
22.2
DUF2341 domain-containing protein
compare
MMP
_RS09055
+2.4
3.9
CAP domain-containing protein
compare
MMP
_RS01275
+2.3
16.1
class III signal peptide-containing protein
compare
MMP
_RS08940
+2.3
4.7
MetS family NSS transporter small subunit
compare
MMP
_RS05635
+2.2
15.6
NAD-dependent epimerase/dehydratase family protein
compare
MMP
_RS03125
+2.2
13.5
cell division ATPase MinD
compare
MMP
_RS07330
+2.1
19.7
membrane protein
compare
MMP
_RS01085
+2.0
5.4
hypothetical protein
compare
MMP
_RS01195
+2.0
3.7
metalloregulator ArsR/SmtB family transcription factor
compare
MMP
_RS08615
+1.9
2.8
flagellar protein G
compare
MMP
_RS06035
+1.9
9.5
glycosyltransferase family 2 protein
compare
MMP
_RS09045
+1.6
9.9
class III signal peptide-containing protein
compare
MMP
_RS00570
+1.5
1.7
protein-L-isoaspartate O-methyltransferase
compare
MMP
_RS05580
+1.5
12.1
glycosyltransferase
compare
MMP
_RS06720
+1.4
5.7
response regulator transcription factor
compare
MMP
_RS05985
+1.4
2.9
hypothetical protein
compare
MMP
_RS01075
+1.3
2.2
hypothetical protein
compare
MMP
_RS00140
+1.3
6.8
DUF192 domain-containing protein
compare
MMP
_RS06715
+1.3
9.1
cache domain-containing protein
compare
MMP
_RS08750
+1.3
1.7
hypothetical protein
compare
MMP
_RS03550
+1.3
1.6
DUF2124 domain-containing protein
compare
MMP
_RS08580
+1.2
4.3
flagellin
compare
MMP
_RS08845
+1.1
4.4
archaellum operon transcriptional activator EarA family protein
compare
MMP
_RS05865
+1.1
1.1
hypothetical protein
compare
MMP
_RS00535
+1.1
1.8
TIGR00375 family protein
compare
MMP
_RS01790
+1.0
2.4
restriction endonuclease
compare
MMP
_RS04125
+1.0
2.3
TetR/AcrR family transcriptional regulator
compare
MMP
_RS08830
+1.0
3.2
GTP-binding protein
compare
MMP
_RS08400
+0.9
1.4
MJ0307 family thioredoxin
compare
MMP
_RS03535
+0.9
1.5
helix-turn-helix transcriptional regulator
compare
MMP
_RS07345
+0.9
2.5
glycine synthesis protein GlyXL
(from data)
compare
MMP
_RS03625
+0.9
2.6
cell wall-binding protein
compare
MMP
_RS04410
+0.9
1.7
hypothetical protein
compare
MMP
_RS05965
+0.9
1.4
carboxymuconolactone decarboxylase family protein
compare
MMP
_RS05785
+0.9
5.9
ATP-binding protein
compare
MMP
_RS08850
+0.8
6.3
hypothetical protein
compare
MMP
_RS00345
+0.8
3.8
DUF2666 domain-containing protein
compare
MMP
_RS06600
+0.8
0.7
class III signal peptide-containing protein
compare
MMP
_RS01255
+0.8
1.8
amino acid ABC transporter ATP-binding protein
compare
MMP
_RS02545
+0.8
1.7
hypothetical protein
compare
MMP
_RS01125
+0.8
2.4
thioredoxin family protein
compare
MMP
_RS01655
+0.8
1.2
DsrE family protein
compare
MMP
_RS03305
+0.7
3.5
winged helix-turn-helix domain-containing protein
compare
MMP
_RS08770
+0.7
3.1
hypothetical protein
compare
MMP
_RS03120
+0.7
2.0
hypothetical protein
compare
MMP
_RS06935
+0.7
2.1
protease complex subunit PrcB family protein
compare
MMP
_RS08410
+0.7
1.7
DUF169 domain-containing protein
compare
MMP
_RS02530
+0.7
1.8
type II toxin-antitoxin system mRNA interferase toxin RelE/StbE family
compare
MMP
_RS08395
+0.7
1.7
DsrE family protein
compare
MMP
_RS07775
+0.7
1.6
alanine dehydrogenase
compare
MMP
_RS01570
+0.7
1.2
hypothetical protein
compare
MMP
_RS01870
+0.7
1.8
acyl-CoA dehydratase activase
compare
MMP
_RS00965
+0.7
1.0
hypothetical protein
compare
MMP
_RS08285
+0.6
2.7
serine/threonine protein kinase
compare
MMP
_RS02020
+0.6
1.3
gamma-glutamylcyclotransferase
compare
MMP
_RS01490
+0.6
1.8
tetratricopeptide repeat protein
compare
MMP
_RS07800
+0.6
2.2
ATP-binding cassette domain-containing protein
compare
MMP
_RS00690
+0.6
1.9
MBL fold metallo-hydrolase
compare
MMP
_RS07900
+0.6
1.0
YeeE/YedE family protein
compare
MMP
_RS08080
+0.6
2.0
YchF/TatD family DNA exonuclease
compare
MMP
_RS08610
+0.6
3.2
flagellar protein F
compare
MMP
_RS01135
+0.6
2.8
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS04255
+0.6
1.7
coenzyme F420 hydrogenase subunit beta
compare
MMP
_RS00090
+0.6
1.3
CoA-binding protein
compare
MMP
_RS08265
+0.6
2.9
rubrerythrin
compare
MMP
_RS07905
+0.5
2.1
DUF3343 domain-containing protein
compare
MMP
_RS03080
+0.5
0.9
universal stress protein
compare
MMP
_RS01110
+0.5
0.8
FmdE family protein
compare
MMP
_RS01185
+0.5
1.9
hypothetical protein
compare
MMP
_RS08980
+0.5
1.7
hypothetical protein
compare
MMP
_RS01260
+0.5
2.2
HDIG domain-containing protein
compare
MMP
_RS01320
+0.5
1.7
hypothetical protein
compare
MMP
_RS08290
+0.5
2.0
DNA-binding protein Alba
compare
MMP
_RS04610
+0.5
1.1
Dabb family protein
compare
MMP
_RS01300
+0.5
3.9
hypothetical protein
compare
MMP
_RS02540
+0.5
1.4
DUF2283 domain-containing protein
compare
MMP
_RS06970
+0.5
2.0
ribosomal protein S18-alanine N-acetyltransferase
compare
MMP
_RS06990
+0.5
2.3
CatB-related O-acetyltransferase
compare
MMP
_RS07550
+0.5
1.9
L-serine ammonia-lyase iron-sulfur-dependent subunit alpha
compare
MMP
_RS06140
+0.5
1.2
radical SAM protein
compare
MMP
_RS04060
+0.5
1.5
hypothetical protein
compare
MMP
_RS08665
+0.5
3.6
hypothetical protein
compare
MMP
_RS00710
+0.5
1.1
hypothetical protein
compare
MMP
_RS02255
+0.5
1.6
hypothetical protein
compare
MMP
_RS01675
+0.5
0.9
hypothetical protein
compare
MMP
_RS06100
+0.5
2.1
GNAT family N-acetyltransferase
compare
MMP
_RS08485
+0.5
2.8
tungstate ABC transporter substrate-binding protein WtpA
compare
MMP
_RS07765
+0.5
2.0
sodium:alanine symporter family protein
compare
MMP
_RS06385
+0.5
1.2
hypothetical protein
compare
MMP
_RS02260
+0.5
1.9
lactate utilization protein
compare
MMP
_RS08490
+0.5
3.2
hypothetical protein
compare
MMP
_RS00295
+0.5
1.1
gamma carbonic anhydrase family protein
compare
MMP
_RS07985
+0.5
3.2
nitroreductase family protein
compare
MMP
_RS08280
+0.5
1.3
proteasome assembly chaperone family protein
compare
MMP
_RS00540
+0.5
2.2
PLP-dependent aminotransferase family protein
compare
MMP
_RS06090
+0.5
1.4
iron ABC transporter permease
compare
MMP
_RS04600
+0.4
3.4
tetratricopeptide repeat protein
compare
MMP
_RS05715
+0.4
1.7
hypothetical protein
compare
MMP
_RS08230
+0.4
1.6
50S ribosomal protein L11 methyltransferase
compare
MMP
_RS02295
+0.4
2.8
TatD family hydrolase
compare
MMP
_RS02385
+0.4
2.5
ATP-binding protein
compare
MMP
_RS07805
+0.4
1.9
ABC transporter permease
compare
MMP
_RS06050
+0.4
1.5
ZIP family metal transporter
compare
MMP
_RS02770
+0.4
1.2
hypothetical protein
compare
MMP
_RS00480
+0.4
1.4
hypothetical protein
compare
MMP
_RS06115
+0.4
2.0
class II aldolase/adducin family protein
compare
MMP
_RS01440
+0.4
1.1
NAAT family transporter
compare
MMP
_RS08250
+0.4
1.7
pyruvate kinase
compare
MMP
_RS03845
+0.4
1.5
DNA adenine methylase
compare
MMP
_RS03900
+0.4
1.5
minichromosome maintenance protein MCM
compare
MMP
_RS06760
+0.4
0.5
hypothetical protein
compare
MMP
_RS02745
+0.4
1.1
hypothetical protein
compare
MMP
_RS07640
+0.4
1.6
mechanosensitive ion channel family protein
compare
MMP
_RS06015
+0.4
0.8
flavodoxin family protein
compare
MMP
_RS07570
+0.4
1.9
nucleotidyltransferase family protein
compare
MMP
_RS02730
+0.4
1.7
ModD protein
compare
MMP
_RS08385
+0.4
1.0
ABC transporter substrate-binding protein
compare
MMP
_RS00930
+0.4
1.0
ABC transporter permease
compare
MMP
_RS01770
+0.4
2.0
ATP-dependent endonuclease
compare
MMP
_RS03530
+0.4
1.8
tetratricopeptide repeat protein
compare
MMP
_RS02425
+0.4
1.3
hypothetical protein
compare
MMP
_RS08700
+0.4
2.8
hypothetical protein
compare
MMP
_RS01630
+0.4
2.3
N-glycosylase/DNA lyase
compare
MMP
_RS01635
+0.4
1.3
thiamine pyrophosphate-dependent enzyme
compare
MMP
_RS05975
+0.4
0.6
hypothetical protein
compare
MMP
_RS07910
+0.4
1.4
NAD(P)H-hydrate dehydratase
compare
MMP
_RS00655
+0.4
1.2
hypothetical protein
compare
MMP
_RS05910
+0.4
0.7
cell division ATPase MinD
compare
MMP
_RS04990
+0.4
1.2
aquaporin family protein
compare
MMP
_RS02635
+0.4
1.4
class I SAM-dependent methyltransferase
compare
MMP
_RS02625
+0.4
1.1
ribonuclease H-like domain-containing protein
compare
MMP
_RS02320
+0.4
1.8
glutamate-cysteine ligase family protein
compare
MMP
_RS01140
+0.4
0.7
molybdate ABC transporter permease subunit
compare
MMP
_RS04390
+0.4
1.5
hypothetical protein
compare
MMP
_RS08775
+0.4
1.8
methanogenesis marker 9 domain-containing protein
compare
MMP
_RS01070
+0.4
2.1
hypothetical protein
compare
MMP
_RS08405
+0.4
2.1
MFS transporter
compare
MMP
_RS01215
+0.4
1.5
sodium/proline symporter PutP
compare
MMP
_RS08205
+0.4
2.0
stage II sporulation protein M
compare
MMP
_RS00640
+0.4
1.2
ABC transporter substrate-binding protein
compare
MMP
_RS07715
+0.3
1.8
YfcE family phosphodiesterase
compare
MMP
_RS04290
+0.3
1.0
4Fe-4S binding protein
compare
MMP
_RS08130
+0.3
2.3
IGHMBP2 family helicase
compare
MMP
_RS07615
+0.3
1.4
energy-coupling factor ABC transporter permease
compare
MMP
_RS08835
+0.3
1.7
H(2)-dependent methylenetetrahydromethanopterin dehydrogenase-related protein
compare
MMP
_RS00555
+0.3
0.8
MFS transporter
compare
MMP
_RS05780
+0.3
1.6
multidrug resistance efflux transporter family protein
compare
MMP
_RS06965
+0.3
1.7
TIGR04014 family B12-binding domain/radical SAM domain-containing protein
compare
MMP
_RS00865
+0.3
1.1
SLC13 family permease
compare
MMP
_RS08620
+0.3
1.4
flagellar accessory protein FlaH
compare
MMP
_RS04995
+0.3
1.4
hypothetical protein
compare
MMP
_RS06170
+0.3
1.4
ABC transporter ATP-binding protein
compare
MMP
_RS08160
+0.3
1.8
EamA family transporter
compare
MMP
_RS03060
+0.3
0.8
MBL fold metallo-hydrolase
compare
MMP
_RS05325
+0.3
1.0
hypothetical protein
compare
MMP
_RS02455
+0.3
1.1
hypothetical protein
compare
MMP
_RS07770
+0.3
1.2
alanine racemase
compare
MMP
_RS01660
+0.3
0.5
hypothetical protein
compare
MMP
_RS04530
+0.3
1.3
ABC transporter permease
compare
MMP
_RS00235
+0.3
1.4
HDIG domain-containing protein
compare
MMP
_RS00935
+0.3
1.5
MATE family efflux transporter
compare
MMP
_RS08640
+0.3
2.5
TIM barrel protein
compare
MMP
_RS07590
+0.3
1.9
hypothetical protein
compare
MMP
_RS00905
+0.3
0.9
phenylacetate--CoA ligase
compare
MMP
_RS06665
+0.3
1.3
glycogen/starch synthase
compare
MMP
_RS05595
+0.3
1.7
imidazole glycerol phosphate synthase subunit HisH
compare
MMP
_RS01690
+0.3
1.4
indolepyruvate ferredoxin oxidoreductase subunit alpha
compare
MMP
_RS03070
+0.3
0.9
AzlD domain-containing protein
compare
MMP
_RS08325
+0.3
2.5
hypothetical protein
compare
MMP
_RS02710
+0.3
1.4
formylmethanofuran dehydrogenase subunit B
compare
MMP
_RS06200
+0.3
0.9
M24 family metallopeptidase
compare
MMP
_RS02610
+0.3
0.6
DUF2162 domain-containing protein
compare
MMP
_RS01685
+0.3
0.9
indolepyruvate oxidoreductase subunit beta
compare
MMP
_RS06175
+0.3
1.8
winged helix-turn-helix transcriptional regulator
compare
MMP
_RS06465
+0.3
1.7
NERD domain-containing protein
compare
MMP
_RS04220
+0.3
1.3
queuosine precursor transporter
compare
MMP
_RS03355
+0.3
1.3
beta-propeller domain-containing protein
compare
MMP
_RS02830
+0.3
1.0
DUF432 domain-containing protein
compare
MMP
_RS09060
+0.3
2.0
hypothetical protein
compare
MMP
_RS02700
+0.3
0.8
formylmethanofuran dehydrogenase subunit C
compare
MMP
_RS03565
+0.3
1.0
uracil phosphoribosyltransferase
compare
MMP
_RS08480
+0.3
2.3
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS08625
+0.3
1.9
type II/IV secretion system ATPase subunit
compare
MMP
_RS03545
+0.3
1.2
ATP-binding protein
compare
MMP
_RS02025
+0.3
1.3
DNA-directed DNA polymerase
compare
MMP
_RS08260
+0.3
1.9
DUF99 family protein
compare
MMP
_RS03965
+0.3
0.7
hypothetical protein
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis S2 in rich t2 experiments