Experiment set3IT009 for Rhodospirillum rubrum S1H

Hexanoic acid 21 mM; anaerobic

Group: carbon source
Media: MELiSSA_defined_50mMCarbonate_noCarbon + Hexanoic acid (21 mM)
Culturing: Rubrum_ML4a, flask, Anaerobic, at 28 (C), shaken=215 rpm
Growth: about 5.8 generations
By: Baptiste on 2/1/19
Media components: 0.051 mM Manganese (II) chloride tetrahydrate, 0.0811 mM Magnesium Sulfate Heptahydrate, 3.801 mM Sodium sulfate, 35.33 mM Ammonium chloride, 0.34 mM Calcium chloride dihydrate, 0.068 mM EDTA, 3.601 mM Potassium phosphate monobasic, 0.072 mM Iron (II) sulfate heptahydrate, 50 mM Sodium bicarbonate, 100.4 mM 3-(N-morpholino)propanesulfonic acid, 2.985 mM Potassium phosphate dibasic, 1.902 uM Nickel (II) sulfate hexahydrate, 0.348 uM Zinc sulfate heptahydrate, 0.02 uM Copper (II) sulfate pentahydrate, 1.623 uM Boric Acid, 0.207 uM Sodium Molybdate Dihydrate, 0.06 uM biotin

Specific Phenotypes

For 2 genes in this experiment

For carbon source Hexanoic acid in Rhodospirillum rubrum S1H

For carbon source Hexanoic acid across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
(2S)-ethylmalonyl-CoA biosynthesis	4	4	1
crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered)	14	6	2
ethylmalonyl-CoA pathway	11	8	1