Experiment set3IT009 for Herbaspirillum seropedicae SmR1

L-mannose carbon source

Group: carbon source
Media: NFbHP_noCarbon + L-mannose (5 g/L), pH=6.5
Culturing: HerbieS_ML4, flask, Aerobic, at 30 (C), shaken=120 rpm
Growth: about 5.6 generations
By: Thalita Tuleski on 19-May-16

Specific Phenotypes

For 6 genes in this experiment

For carbon source L-mannose in Herbaspirillum seropedicae SmR1

For carbon source L-mannose across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial Chemotaxis	1
Coenzyme A Biosynthesis	1
D-ribose utilization	1
Mannose Metabolism	1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	1
Xylose utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pantothenate and CoA biosynthesis
• Pentose and glucuronate interconversions
• Fructose and mannose metabolism
• Valine, leucine and isoleucine biosynthesis
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
phosphopantothenate biosynthesis II	3	1	1
phosphopantothenate biosynthesis I	4	4	1
L-valine biosynthesis	4	4	1
D-xylose degradation to ethylene glycol (engineered)	4	1	1
superpathway of coenzyme A biosynthesis III (mammals)	5	5	1
D-xylose degradation V	5	4	1
pyruvate fermentation to isobutanol (engineered)	5	4	1
D-xylose degradation III	5	3	1
D-xylose degradation VI	5	3	1
L-isoleucine biosynthesis IV	6	4	1
L-isoleucine biosynthesis I (from threonine)	7	7	1
L-isoleucine biosynthesis III	7	4	1
D-xylose degradation IV	7	3	1
L-isoleucine biosynthesis II	8	6	1
superpathway of branched chain amino acid biosynthesis	17	17	2
superpathway of coenzyme A biosynthesis I (bacteria)	9	9	1
superpathway of coenzyme A biosynthesis II (plants)	10	8	1
superpathway of L-isoleucine biosynthesis I	13	13	1
superpathway of L-threonine metabolism	18	13	1
superpathway of pentose and pentitol degradation	42	19	1