Experiment set3H46 for Pseudomonas stutzeri RCH2

LB with benzoic acid 0.4 mg/ml

Group: stress
Media: LB + benzoic acid (0.4 mg/ml)
Culturing: psRCH2_ML7, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Kelly on 6/24/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 557 C7,C8

Specific Phenotypes

For 2 genes in this experiment

For stress benzoic acid in Pseudomonas stutzeri RCH2

For stress benzoic acid across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Benzoate degradation via hydroxylation
• Styrene degradation
• Butanoate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
3-oxoadipate degradation	2	2	1
catechol degradation III (ortho-cleavage pathway)	6	6	1
superpathway of salicylate degradation	7	6	1
4-methylcatechol degradation (ortho cleavage)	7	5	1
L-glutamate degradation XI (reductive Stickland reaction)	7	4	1
aromatic compounds degradation via β-ketoadipate	9	7	1
L-glutamate degradation V (via hydroxyglutarate)	10	7	1
toluene degradation III (aerobic) (via p-cresol)	11	4	1
mandelate degradation to acetyl-CoA	18	9	1
superpathway of aerobic toluene degradation	30	7	1
superpathway of aromatic compound degradation via 3-oxoadipate	35	11	1