Experiment set3H40 for Pseudomonas stutzeri RCH2

LB with Sodium Chlorate 75 mM

Group: stress
Media: LB + Sodium Chlorate (75 mM)
Culturing: psRCH2_ML7, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Kelly on 6/24/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 556 C5,C6

Specific Phenotypes

For 26 genes in this experiment

For stress Sodium Chlorate in Pseudomonas stutzeri RCH2

For stress Sodium Chlorate across organisms

SEED Subsystems

Subsystem	#Specific
Control of cell elongation - division cycle in Bacilli	1
DNA Repair Base Excision	1
DNA repair, UvrABC system	1
DNA repair, bacterial RecFOR pathway	1
Flavodoxin	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
LMPTP YfkJ cluster	1
Multidrug Resistance Efflux Pumps	1
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes	1
Na+ translocating decarboxylases and related biotin-dependent enzymes	1
Nudix proteins (nucleoside triphosphate hydrolases)	1
Oxidative stress	1
Photorespiration (oxidative C2 cycle)	1
Pyruvate metabolism I: anaplerotic reactions, PEP	1
Queuosine-Archaeosine Biosynthesis	1
Restriction-Modification System	1
Terminal cytochrome C oxidases	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism
• Oxidative phosphorylation
• Glutamate metabolism
• Tryptophan metabolism
• Pyruvate metabolism
• Methane metabolism
• Nitrogen metabolism
• mTOR signaling pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamine biosynthesis I	1	1	1
superoxide radicals degradation	2	2	1
ammonia assimilation cycle I	2	2	1
arsenite to oxygen electron transfer	2	1	1
ammonia assimilation cycle II	2	1	1
methanol oxidation to formaldehyde IV	2	1	1
ammonia assimilation cycle III	3	3	1
ethanol degradation IV	3	3	1
L-aspartate degradation III (anaerobic)	3	2	1
L-aspartate degradation II (aerobic)	3	2	1
superpathway of ammonia assimilation (plants)	3	2	1
arsenite to oxygen electron transfer (via azurin)	3	1	1
reactive oxygen species degradation	4	4	1
aerobic respiration I (cytochrome c)	4	3	1
aerobic respiration II (cytochrome c) (yeast)	4	3	1
Fe(II) oxidation	6	3	1
methylgallate degradation	6	2	1
L-glutamate and L-glutamine biosynthesis	7	5	1
protocatechuate degradation I (meta-cleavage pathway)	8	3	1
L-arginine biosynthesis II (acetyl cycle)	10	10	1
superpathway of vanillin and vanillate degradation	10	3	1
syringate degradation	12	3	1