Experiment set3H30 for Phaeobacter inhibens DSM 17395
N-Acetyl-D-Glucosamine carbon source
Group: carbon sourceMedia: DinoMM_noCarbon + N-Acetyl-D-Glucosamine (20 mM), pH=7
Culturing: Phaeo_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jordan on 8/5/2013
Media components: 20 g/L Sea salts, 0.3 g/L Ammonium Sulfate, 0.1 g/L Potassium phosphate monobasic, Wolfe's mineral mix (0.003 g/L Magnesium Sulfate Heptahydrate, 0.0015 g/L Nitrilotriacetic acid, 0.001 g/L Sodium Chloride, 0.0005 g/L Manganese (II) sulfate monohydrate, 0.0001 g/L Cobalt chloride hexahydrate, 0.0001 g/L Zinc sulfate heptahydrate, 0.0001 g/L Calcium chloride dihydrate, 0.0001 g/L Iron (II) sulfate heptahydrate, 2.5e-05 g/L Nickel (II) chloride hexahydrate, 2e-05 g/L Aluminum potassium sulfate dodecahydrate, 1e-05 g/L Copper (II) sulfate pentahydrate, 1e-05 g/L Boric Acid, 1e-05 g/L Sodium Molybdate Dihydrate, 0.0003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.05 mg/L Pyridoxine HCl, 0.025 mg/L 4-Aminobenzoic acid, 0.025 mg/L Lipoic acid, 0.025 mg/L Nicotinic Acid, 0.025 mg/L Riboflavin, 0.025 mg/L Thiamine HCl, 0.025 mg/L calcium pantothenate, 0.01 mg/L biotin, 0.01 mg/L Folic Acid, 0.0005 mg/L Cyanocobalamin)
Growth plate: 620 F5,F6
Specific Phenotypes
For 12 genes in this experiment
For carbon source N-Acetyl-D-Glucosamine in Phaeobacter inhibens DSM 17395
For carbon source N-Acetyl-D-Glucosamine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Chitin and N-acetylglucosamine utilization | 7 |
| UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis | 1 |
| cAMP signaling in bacteria | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| taurine degradation I | 1 | 1 | 1 |
| N-acetylglucosamine degradation II | 3 | 3 | 2 |
| N-acetylglucosamine degradation I | 2 | 2 | 1 |
| hypotaurine degradation | 3 | 3 | 1 |
| chitin derivatives degradation | 8 | 3 | 2 |
| superpathway of taurine degradation | 6 | 4 | 1 |
| superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation | 6 | 2 | 1 |
| UDP-N-acetyl-D-galactosamine biosynthesis II | 7 | 5 | 1 |
| chitin degradation I (archaea) | 7 | 1 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 15 | 1 |