Experiment set3H16 for Shewanella oneidensis MR-1
Sodium L-Lactate carbon source replicate 2
Group: carbon sourceMedia: ShewMM_noCarbon + Sodium L-Lactate (20 mM), pH=7
Culturing: MR1_ML3, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 5.5 generations
By: Adam on 8/18/2013
Media components: 1.5 g/L Ammonium chloride, 1.75 g/L Sodium Chloride, 0.61 g/L Magnesium chloride hexahydrate, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 7 genes in this experiment
For carbon source Sodium L-Lactate in Shewanella oneidensis MR-1
For carbon source Sodium L-Lactate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Isobutyryl-CoA to Propionyl-CoA Module | 3 |
| Cysteine Biosynthesis | 2 |
| Isoleucine degradation | 2 |
| Valine degradation | 2 |
| Leucine Degradation and HMG-CoA Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Valine, leucine and isoleucine degradation
- Fatty acid metabolism
- Geraniol degradation
- Benzoate degradation via CoA ligation
- Propanoate metabolism
- Biosynthesis of plant hormones
- Fatty acid elongation in mitochondria
- Synthesis and degradation of ketone bodies
- Cysteine metabolism
- Lysine degradation
- Benzoate degradation via hydroxylation
- Tryptophan metabolism
- Selenoamino acid metabolism
- Inositol phosphate metabolism
- alpha-Linolenic acid metabolism
- Pyruvate metabolism
- 1- and 2-Methylnaphthalene degradation
- Ethylbenzene degradation
- Butanoate metabolism
- Terpenoid biosynthesis
- Brassinosteroid biosynthesis
- Sulfur metabolism
- Caprolactam degradation
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of terpenoids and steroids
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: