Experiment set3H15 for Phaeobacter inhibens DSM 17395
Sodium D-Lactate carbon source
Group: carbon sourceMedia: DinoMM_noCarbon + Sodium D-Lactate (20 mM), pH=7
Culturing: Phaeo_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jordan on 8/5/2013
Media components: 20 g/L Sea salts, 0.3 g/L Ammonium Sulfate, 0.1 g/L Potassium phosphate monobasic, Wolfe's mineral mix (0.003 g/L Magnesium Sulfate Heptahydrate, 0.0015 g/L Nitrilotriacetic acid, 0.001 g/L Sodium Chloride, 0.0005 g/L Manganese (II) sulfate monohydrate, 0.0001 g/L Cobalt chloride hexahydrate, 0.0001 g/L Zinc sulfate heptahydrate, 0.0001 g/L Calcium chloride dihydrate, 0.0001 g/L Iron (II) sulfate heptahydrate, 2.5e-05 g/L Nickel (II) chloride hexahydrate, 2e-05 g/L Aluminum potassium sulfate dodecahydrate, 1e-05 g/L Copper (II) sulfate pentahydrate, 1e-05 g/L Boric Acid, 1e-05 g/L Sodium Molybdate Dihydrate, 0.0003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.05 mg/L Pyridoxine HCl, 0.025 mg/L 4-Aminobenzoic acid, 0.025 mg/L Lipoic acid, 0.025 mg/L Nicotinic Acid, 0.025 mg/L Riboflavin, 0.025 mg/L Thiamine HCl, 0.025 mg/L calcium pantothenate, 0.01 mg/L biotin, 0.01 mg/L Folic Acid, 0.0005 mg/L Cyanocobalamin)
Growth plate: 620 B7,B8
Specific Phenotypes
For 9 genes in this experiment
For carbon source Sodium D-Lactate in Phaeobacter inhibens DSM 17395
For carbon source Sodium D-Lactate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glycolate, glyoxylate interconversions | 3 |
| Photorespiration (oxidative C2 cycle) | 3 |
| ABC transporter branched-chain amino acid (TC 3.A.1.4.1) | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycolate and glyoxylate degradation II | 2 | 2 | 1 |
| glycolate and glyoxylate degradation III | 3 | 1 | 1 |
| glycolate and glyoxylate degradation I | 4 | 2 | 1 |
| superpathway of glycol metabolism and degradation | 7 | 4 | 1 |
| photorespiration I | 9 | 7 | 1 |
| photorespiration III | 9 | 7 | 1 |
| photorespiration II | 10 | 7 | 1 |