Experiment set3H14 for Marinobacter adhaerens HP15

Compare to:

marine broth with methylglyoxal 0.008 vol%

Group: stress
Media: marine_broth_2216 + methylglyoxal (0.008 vol%)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jordan on 9/10/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate

Specific Phenotypes

For 31 genes in this experiment

For stress methylglyoxal in Marinobacter adhaerens HP15

For stress methylglyoxal across organisms

SEED Subsystems

Subsystem #Specific
DNA-replication 4
DNA repair, bacterial RecFOR pathway 3
DNA repair, UvrABC system 2
DNA repair, bacterial 2
Cysteine Biosynthesis 1
DNA Repair Base Excision 1
DNA repair, bacterial DinG and relatives 1
Fatty Acid Biosynthesis FASII 1
Glutathione-dependent pathway of formaldehyde detoxification 1
Glutathione: Non-redox reactions 1
Glutathione: Redox cycle 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Methionine Biosynthesis 1
Methionine Degradation 1
Methylglyoxal Metabolism 1
Multidrug Resistance Efflux Pumps 1
Phosphate metabolism 1
Protein chaperones 1
Protein degradation 1
Thioredoxin-disulfide reductase 1
Type IV pilus 1
pVir Plasmid of Campylobacter 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
methylglyoxal degradation I 3 2 2
methylglyoxal degradation VIII 3 2 2
phospholipid remodeling (phosphatidate, yeast) 2 1 1
palmitoleate biosynthesis III (cyanobacteria) 2 1 1
formaldehyde oxidation II (glutathione-dependent) 3 3 1
oleate biosynthesis III (cyanobacteria) 3 2 1
glutathione-peroxide redox reactions 3 2 1
CDP-diacylglycerol biosynthesis II 4 4 1
gondoate biosynthesis (anaerobic) 4 4 1
CDP-diacylglycerol biosynthesis I 4 4 1
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) 12 9 3
fatty acid biosynthesis initiation (mitochondria) 4 3 1
superpathway of methylglyoxal degradation 8 4 2
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) 9 9 2
oleate biosynthesis IV (anaerobic) 14 13 3
palmitate biosynthesis III 29 21 6
fatty acid elongation -- saturated 5 5 1
superpathway of fatty acid biosynthesis initiation 5 5 1
tetradecanoate biosynthesis (mitochondria) 25 17 5
CDP-diacylglycerol biosynthesis III 5 3 1
phosphatidate biosynthesis (yeast) 5 3 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 6
superpathway of fatty acid biosynthesis I (E. coli) 16 16 3
8-amino-7-oxononanoate biosynthesis I 11 9 2
superpathway of fatty acids biosynthesis (E. coli) 53 51 9
(5Z)-dodecenoate biosynthesis I 6 6 1
phosphatidylglycerol biosynthesis I 6 6 1
phosphatidylglycerol biosynthesis II 6 6 1
superpathway of phospholipid biosynthesis III (E. coli) 12 11 2
stearate biosynthesis II (bacteria and plants) 6 5 1
(5Z)-dodecenoate biosynthesis II 6 5 1
stearate biosynthesis IV 6 4 1
superpathway of stearidonate biosynthesis (cyanobacteria) 6 2 1
palmitoyl ethanolamide biosynthesis 6 2 1
superpathway of fatty acid biosynthesis II (plant) 43 38 7
superpathway of unsaturated fatty acids biosynthesis (E. coli) 20 20 3
stigma estolide biosynthesis 7 2 1
diacylglycerol and triacylglycerol biosynthesis 7 2 1
biotin biosynthesis I 15 13 2
anandamide biosynthesis II 8 2 1
streptorubin B biosynthesis 34 20 4
anandamide biosynthesis I 12 4 1
superpathway of C1 compounds oxidation to CO2 12 4 1
superpathway of cardiolipin biosynthesis (bacteria) 13 10 1
superpathway of phospholipid biosynthesis II (plants) 28 10 2
plasmalogen biosynthesis I (aerobic) 16 2 1
mupirocin biosynthesis 26 2 1
mycolate biosynthesis 205 20 4
superpathway of mycolate biosynthesis 239 21 4