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Experiment set39S405 for
Burkholderia phytofirmans PsJN
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24hr PHYTO8 MOI 1
200 most detrimental genes:
gene
name
fitness
t score
description
BPHYT
_RS11705
+3.3
3.0
hypothetical protein
compare
BPHYT
_RS19565
+2.8
21.6
gamma-glutamyl:cysteine ligase
compare
BPHYT
_RS33820
+2.8
25.9
histidine kinase
compare
BPHYT
_RS19570
+2.8
25.6
sodium/hydrogen exchanger
compare
BPHYT
_RS02595
+2.7
7.7
sulfurtransferase
compare
BPHYT
_RS14140
+2.5
6.6
phosphate transporter permease subunit PstC
compare
BPHYT
_RS14400
+2.4
5.4
anti-sigma factor
compare
BPHYT
_RS06175
+2.4
7.7
LPS biosynthesis
compare
BPHYT
_RS09330
+2.4
19.6
phytoene synthase
compare
BPHYT
_RS12985
+2.4
2.3
amino acid acetyltransferase
compare
BPHYT
_RS33920
+2.3
13.3
type II citrate synthase
compare
BPHYT
_RS05560
+2.2
11.7
Fis family transcriptional regulator
compare
BPHYT
_RS05575
+2.1
15.8
Fis family transcriptional regulator
compare
BPHYT
_RS04945
+2.1
18.1
membrane protein
compare
BPHYT
_RS12845
+2.1
16.0
ATPase
compare
BPHYT
_RS04950
+2.0
16.4
hypothetical protein
compare
BPHYT
_RS03400
+2.0
1.2
ATP-dependent DNA helicase RecG
compare
BPHYT
_RS25935
+2.0
3.9
type III secretion protein
compare
BPHYT
_RS05155
+2.0
7.9
acetyltransferase GCN5
compare
BPHYT
_RS07840
+1.9
2.5
muconate cycloisomerase
compare
BPHYT
_RS02905
+1.9
17.7
RNA polymerase subunit sigma-54
compare
BPHYT
_RS23910
+1.9
5.2
phosphoribosyl-dephospho-CoA transferase
compare
BPHYT
_RS06985
+1.9
9.3
fumarate hydratase
compare
BPHYT
_RS06075
+1.8
9.9
hypothetical protein
compare
BPHYT
_RS13645
+1.8
16.5
threonine--tRNA ligase
compare
BPHYT
_RS12725
+1.8
14.7
regulator
compare
BPHYT
_RS05590
+1.8
13.1
trehalose-6-phosphate synthase
compare
BPHYT
_RS15890
+1.7
1.5
3-hydroxy-2-methylbutyryl-CoA dehydrogenase
compare
BPHYT
_RS12465
+1.7
3.7
inositol monophosphatase
compare
BPHYT
_RS21330
+1.6
2.4
hypothetical protein
compare
BPHYT
_RS08945
+1.6
11.2
hypothetical protein
compare
BPHYT
_RS09660
+1.6
10.4
trehalose-6-phosphate synthase
compare
BPHYT
_RS28065
+1.6
1.4
hypothetical protein
compare
BPHYT
_RS28075
+1.6
2.1
cytochrome B561
compare
BPHYT
_RS14395
+1.6
6.0
sugar dehydratase
compare
BPHYT
_RS14485
+1.6
5.4
ribonuclease E
compare
BPHYT
_RS29855
+1.6
1.7
protein-tyrosine-phosphatase
compare
BPHYT
_RS32395
+1.5
1.1
hypothetical protein
compare
BPHYT
_RS25870
+1.5
0.9
type III secretion system ATPase
compare
BPHYT
_RS11815
+1.5
1.7
alcohol dehydrogenase
compare
BPHYT
_RS09500
+1.5
6.7
glucose-6-phosphate isomerase
compare
BPHYT
_RS19920
+1.5
2.3
peptidyl-prolyl cis-trans isomerase
compare
BPHYT
_RS06530
+1.5
1.6
membrane protein
compare
BPHYT
_RS35770
+1.5
1.8
glyoxalase
compare
BPHYT
_RS08235
+1.4
0.6
nucleoprotein, Hfg, host factor I
compare
BPHYT
_RS18655
+1.4
5.9
3-oxoacyl-ACP synthase
compare
BPHYT
_RS13200
+1.4
3.0
molybdopterin binding oxidoreductase
compare
BPHYT
_RS16335
+1.4
3.5
membrane protein
compare
BPHYT
_RS12150
+1.4
10.0
PII uridylyl-transferase
compare
BPHYT
_RS10135
+1.4
1.1
hypothetical protein
compare
BPHYT
_RS05025
+1.4
1.7
sugar ABC transporter substrate-binding protein
compare
BPHYT
_RS16175
+1.3
0.8
hemolysin D
compare
BPHYT
_RS15285
+1.3
3.7
transcription elongation factor GreB
compare
BPHYT
_RS09645
+1.3
2.4
ankyrin
compare
BPHYT
_RS04455
+1.3
0.8
glycosyl transferase
compare
BPHYT
_RS09815
+1.3
1.9
malate synthase
compare
BPHYT
_RS16345
+1.3
3.9
succinyl-CoA ligase [ADP-forming] subunit beta
compare
BPHYT
_RS23355
+1.3
2.1
membrane protein
compare
BPHYT
_RS15325
+1.3
3.7
ribonuclease PH
compare
BPHYT
_RS02765
+1.3
1.4
cytochrome BD oxidase subunit I
compare
BPHYT
_RS14145
+1.3
6.7
phosphate ABC transporter substrate-binding protein
compare
BPHYT
_RS04940
+1.3
1.9
hypothetical protein
compare
BPHYT
_RS26570
+1.3
1.2
5'-nucleotidase
compare
BPHYT
_RS09390
+1.2
1.6
ABC transporter permease
compare
BPHYT
_RS06375
+1.2
2.3
ribonuclease
compare
BPHYT
_RS09160
+1.2
4.3
polyhydroxyalkanoate synthesis repressor PhaR
compare
BPHYT
_RS08765
+1.2
1.0
ABC transporter ATP-binding protein
compare
BPHYT
_RS28995
+1.2
2.3
histidine kinase
compare
BPHYT
_RS31320
+1.2
2.6
hypothetical protein
compare
BPHYT
_RS08645
+1.2
1.6
sulfonate ABC transporter
compare
BPHYT
_RS06390
+1.2
2.5
ribosome-associated protein IOJAP
compare
BPHYT
_RS17095
+1.2
2.6
proline--tRNA ligase
compare
BPHYT
_RS21055
+1.2
1.9
S-formylglutathione hydrolase
compare
BPHYT
_RS11745
+1.2
1.0
(2Fe-2S)-binding protein
compare
BPHYT
_RS23280
+1.2
2.4
phosphate ABC transporter substrate-binding protein
compare
BPHYT
_RS34490
+1.2
1.2
hypothetical protein
compare
BPHYT
_RS30750
+1.2
1.0
GntR family transcriptional regulator
compare
BPHYT
_RS08705
+1.2
3.3
hypothetical protein
compare
BPHYT
_RS28030
+1.2
2.4
methylmalonate-semialdehyde dehydrogenase
compare
BPHYT
_RS10320
+1.2
3.3
hypothetical protein
compare
BPHYT
_RS06070
+1.2
5.8
cytochrome C
compare
BPHYT
_RS02640
+1.2
8.5
cytochrome C oxidase
compare
BPHYT
_RS28985
+1.2
4.1
hypothetical protein
compare
BPHYT
_RS05615
+1.1
1.0
hypothetical protein
compare
BPHYT
_RS29235
+1.1
1.3
cell shape determination protein CcmA
compare
BPHYT
_RS08135
+1.1
1.3
hypothetical protein
compare
BPHYT
_RS07900
+1.1
1.3
hypothetical protein
compare
BPHYT
_RS08405
+1.1
1.6
hypothetical protein
compare
BPHYT
_RS08000
+1.1
2.6
antibiotic biosynthesis monooxygenase
compare
BPHYT
_RS19325
+1.1
1.6
glycine cleavage system H protein
compare
BPHYT
_RS18255
+1.1
1.9
phenylacetate-CoA oxygenase subunit PaaB
compare
BPHYT
_RS03620
+1.1
5.7
poly(A) polymerase
compare
BPHYT
_RS04290
+1.1
0.8
glycosyl transferase family 2
compare
BPHYT
_RS06065
+1.1
6.3
cytochrome C
compare
BPHYT
_RS24255
+1.1
3.2
hypothetical protein
compare
BPHYT
_RS08850
+1.1
0.5
transcriptional regulator
compare
BPHYT
_RS25980
+1.1
1.1
urea carboxylase
compare
BPHYT
_RS15620
+1.1
2.2
peptidyl-prolyl cis-trans isomerase
compare
BPHYT
_RS08510
+1.1
1.4
acyl-CoA dehydrogenase
compare
BPHYT
_RS06350
+1.1
0.8
chemotaxis protein CheY
compare
BPHYT
_RS11540
+1.1
2.2
ureidoglycolate hydrolase
compare
BPHYT
_RS05610
+1.1
6.1
DNA-cytosine methyltransferase
compare
BPHYT
_RS08385
+1.1
1.2
secretion protein
compare
BPHYT
_RS30610
+1.1
2.1
major facilitator transporter
compare
BPHYT
_RS36480
+1.1
1.6
hypothetical protein
compare
BPHYT
_RS16340
+1.1
4.2
succinyl-CoA synthetase subunit alpha
compare
BPHYT
_RS20085
+1.1
1.7
mannosyltransferase
compare
BPHYT
_RS02710
+1.1
1.4
hypothetical protein
compare
BPHYT
_RS02540
+1.1
2.1
hypothetical protein
compare
BPHYT
_RS34185
+1.1
5.6
cold-shock protein
compare
BPHYT
_RS30085
+1.0
1.2
hypothetical protein
compare
BPHYT
_RS04990
+1.0
1.3
hypothetical protein
compare
BPHYT
_RS10435
+1.0
1.8
ThiJ/PfpI domain-containing protein
compare
BPHYT
_RS17640
+1.0
0.9
2-alkenal reductase
compare
BPHYT
_RS11125
+1.0
1.1
alkylhydroperoxidase
compare
BPHYT
_RS06500
+1.0
2.2
LOG family protein
compare
BPHYT
_RS20185
+1.0
1.6
sugar transferase
compare
BPHYT
_RS09590
+1.0
2.0
thioesterase
compare
BPHYT
_RS27295
+1.0
1.4
hypothetical protein
compare
BPHYT
_RS28930
+1.0
1.2
chromosome partitioning protein ParA
compare
BPHYT
_RS19130
+1.0
1.6
acetyltransferase
compare
BPHYT
_RS20880
+1.0
1.2
polyketide cyclase
compare
BPHYT
_RS34530
+1.0
1.9
LysR family transcriptional regulator
compare
BPHYT
_RS09650
+1.0
0.8
hypothetical protein
compare
BPHYT
_RS32240
+1.0
1.1
chromosome replication initiation inhibitor protein
compare
BPHYT
_RS02760
+1.0
1.0
cytochrome BD oxidase subunit II
compare
BPHYT
_RS04500
+1.0
1.6
urease accessory protein UreF
compare
BPHYT
_RS31615
+1.0
1.2
thiamine pyrophosphate protein
compare
BPHYT
_RS26930
+1.0
4.2
mechanosensitive ion channel protein
compare
BPHYT
_RS10995
+1.0
1.7
GntR family transcriptional regulator
compare
BPHYT
_RS06725
+1.0
1.6
MaoC family dehydratase
compare
BPHYT
_RS35500
+1.0
2.4
major facilitator transporter
compare
BPHYT
_RS29250
+1.0
2.5
dihydrodipicolinate synthase
compare
BPHYT
_RS05670
+1.0
1.8
hypothetical protein
compare
BPHYT
_RS06710
+1.0
1.0
hypothetical protein
compare
BPHYT
_RS33885
+1.0
7.4
tRNA pseudouridine synthase A
compare
BPHYT
_RS02530
+1.0
5.5
hypothetical protein
compare
BPHYT
_RS02525
+1.0
4.5
membrane protein
compare
BPHYT
_RS35310
+1.0
1.0
GCN5 family N-acetyltransferase
compare
BPHYT
_RS33695
+1.0
1.9
molybdenum ABC transporter ATPase
compare
BPHYT
_RS10865
+1.0
2.7
transporter
compare
BPHYT
_RS23390
+1.0
1.8
hypothetical protein
compare
BPHYT
_RS11310
+1.0
1.4
LysR family transcriptional regulator
compare
BPHYT
_RS14135
+1.0
5.9
phosphate transporter permease subunit PtsA
compare
BPHYT
_RS36150
+1.0
2.4
hypothetical protein
compare
BPHYT
_RS03625
+1.0
5.1
Histidinol-phosphatase (EC:3.1.3.15)
(from data)
compare
BPHYT
_RS10535
+1.0
3.6
DSBA oxidoreductase
compare
BPHYT
_RS15865
+1.0
1.6
exodeoxyribonuclease VII large subunit
compare
BPHYT
_RS25140
+1.0
2.0
hypothetical protein
compare
BPHYT
_RS23035
+0.9
1.1
hypothetical protein
compare
BPHYT
_RS08565
+0.9
0.5
dihydroorotate dehydrogenase
compare
BPHYT
_RS17440
+0.9
2.2
hypothetical protein
compare
BPHYT
_RS15800
+0.9
2.8
GntR family transcriptional regulator
compare
BPHYT
_RS07570
+0.9
0.9
hypothetical protein
compare
BPHYT
_RS31910
+0.9
1.2
hypothetical protein
compare
BPHYT
_RS14275
+0.9
1.5
hypothetical protein
compare
BPHYT
_RS36570
+0.9
1.5
histone
compare
BPHYT
_RS30995
+0.9
0.4
glucose-1-phosphate thymidylyltransferase
compare
BPHYT
_RS09640
+0.9
4.1
DNAase
compare
BPHYT
_RS20130
+0.9
2.2
diguanylate phosphodiesterase
compare
BPHYT
_RS22910
+0.9
0.4
hypothetical protein
compare
BPHYT
_RS09145
+0.9
6.1
poly-beta-hydroxybutyrate polymerase
compare
BPHYT
_RS16060
+0.9
2.3
ribonucleotide-diphosphate reductase subunit alpha
compare
BPHYT
_RS13170
+0.9
2.6
ATPase
compare
BPHYT
_RS10945
+0.9
1.0
2-deoxy-D-gluconate 3-dehydrogenase
compare
BPHYT
_RS36230
+0.9
0.8
hypothetical protein
compare
BPHYT
_RS02010
+0.9
2.0
copper homeostasis protein CutC
compare
BPHYT
_RS02005
+0.9
1.5
diguanylate phosphodiesterase
compare
BPHYT
_RS11455
+0.9
3.1
manganese transporter
compare
BPHYT
_RS30820
+0.9
2.2
GntR family transcriptional regulator
compare
BPHYT
_RS04140
+0.9
3.4
polysaccharide biosynthesis protein
compare
BPHYT
_RS02600
+0.9
1.1
glutaredoxin
compare
BPHYT
_RS06910
+0.9
1.7
membrane protein
compare
BPHYT
_RS30285
+0.9
1.6
succinate-semialdehyde dehydrogenase
compare
BPHYT
_RS02635
+0.9
5.5
membrane protein
compare
BPHYT
_RS36000
+0.9
1.1
hypothetical protein
compare
BPHYT
_RS05905
+0.9
1.1
hypothetical protein
compare
BPHYT
_RS03460
+0.9
4.2
mammalian cell entry protein
compare
BPHYT
_RS26780
+0.9
3.0
chemotaxis protein CheR
compare
BPHYT
_RS26040
+0.9
1.8
cysteine hydrolase
compare
BPHYT
_RS31225
+0.9
1.9
D-alanyl-D-alanine dipeptidase
compare
BPHYT
_RS30825
+0.9
1.4
extradiol dioxygenase
compare
BPHYT
_RS28670
+0.9
1.0
MFS transporter
compare
BPHYT
_RS12635
+0.9
2.6
translation factor Sua5
compare
BPHYT
_RS03725
+0.9
0.6
serine hydroxymethyltransferase
compare
BPHYT
_RS21160
+0.9
2.5
NAD(P) transhydrogenase subunit beta
compare
BPHYT
_RS22580
+0.9
3.7
transcriptional regulator
compare
BPHYT
_RS23455
+0.9
1.0
phosphorylase
compare
BPHYT
_RS16840
+0.9
1.7
transketolase
compare
BPHYT
_RS07925
+0.9
1.8
glutamine amidotransferase
compare
BPHYT
_RS02660
+0.8
1.3
membrane protein
compare
BPHYT
_RS03665
+0.8
2.0
hypothetical protein
compare
BPHYT
_RS11080
+0.8
2.2
hypothetical protein
compare
BPHYT
_RS05010
+0.8
2.4
RpiR family transcriptional regulator
compare
BPHYT
_RS26005
+0.8
1.7
MarR family transcriptional regulator
compare
BPHYT
_RS16435
+0.8
6.4
alcohol dehydrogenase
compare
BPHYT
_RS23155
+0.8
1.2
Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)
(from data)
compare
BPHYT
_RS35550
+0.8
1.3
nitrile hydratase
compare
BPHYT
_RS24040
+0.8
1.8
amino acid ABC transporter
compare
BPHYT
_RS14855
+0.8
0.4
ADP-L-glycero-D-manno-heptose-6-epimerase
compare
Specific Phenotypes
None in this experiment
For Burkholderia phytofirmans PsJN in phage experiments