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Experiment set39S367 for
Burkholderia phytofirmans PsJN
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36hr PHYTO6 MOI 10
200 most detrimental genes:
gene
name
fitness
t score
description
BPHYT
_RS11705
+4.1
2.9
hypothetical protein
compare
BPHYT
_RS14140
+3.6
6.6
phosphate transporter permease subunit PstC
compare
BPHYT
_RS19570
+3.2
26.6
sodium/hydrogen exchanger
compare
BPHYT
_RS19565
+3.2
16.6
gamma-glutamyl:cysteine ligase
compare
BPHYT
_RS09500
+2.9
17.5
glucose-6-phosphate isomerase
compare
BPHYT
_RS33820
+2.9
27.8
histidine kinase
compare
BPHYT
_RS16710
+2.8
1.3
LysR family transcriptional regulator
compare
BPHYT
_RS06530
+2.8
4.3
membrane protein
compare
BPHYT
_RS04965
+2.6
13.3
ATP-binding protein
compare
BPHYT
_RS14855
+2.5
1.1
ADP-L-glycero-D-manno-heptose-6-epimerase
compare
BPHYT
_RS14400
+2.5
5.7
anti-sigma factor
compare
BPHYT
_RS05575
+2.5
17.1
Fis family transcriptional regulator
compare
BPHYT
_RS18655
+2.4
7.0
3-oxoacyl-ACP synthase
compare
BPHYT
_RS06175
+2.4
5.6
LPS biosynthesis
compare
BPHYT
_RS02595
+2.4
3.4
sulfurtransferase
compare
BPHYT
_RS04945
+2.4
20.6
membrane protein
compare
BPHYT
_RS25935
+2.4
2.2
type III secretion protein
compare
BPHYT
_RS01455
+2.3
3.0
peptide deformylase
compare
BPHYT
_RS04950
+2.3
18.9
hypothetical protein
compare
BPHYT
_RS16675
+2.3
2.7
dihydroorotase
compare
BPHYT
_RS33920
+2.3
17.3
type II citrate synthase
compare
BPHYT
_RS16175
+2.2
1.8
hemolysin D
compare
BPHYT
_RS27890
+2.2
1.2
hypothetical protein
compare
BPHYT
_RS02865
+2.2
1.9
hypothetical protein
compare
BPHYT
_RS05560
+2.2
15.5
Fis family transcriptional regulator
compare
BPHYT
_RS02905
+2.2
19.4
RNA polymerase subunit sigma-54
compare
BPHYT
_RS12550
+2.2
1.5
GTP-binding protein HflX
compare
BPHYT
_RS22835
+2.2
2.9
flagellar motor protein MotA
compare
BPHYT
_RS21905
+2.2
1.8
IclR family transcriptional regulator
compare
BPHYT
_RS05590
+2.1
17.3
trehalose-6-phosphate synthase
compare
BPHYT
_RS08850
+2.1
0.9
transcriptional regulator
compare
BPHYT
_RS01435
+2.1
18.3
LysR family transcriptional regulator
compare
BPHYT
_RS05610
+2.1
13.4
DNA-cytosine methyltransferase
compare
BPHYT
_RS30750
+2.1
2.6
GntR family transcriptional regulator
compare
BPHYT
_RS12845
+2.0
16.4
ATPase
compare
BPHYT
_RS09330
+2.0
15.9
phytoene synthase
compare
BPHYT
_RS04940
+2.0
2.7
hypothetical protein
compare
BPHYT
_RS17035
+1.9
2.1
sugar kinase
compare
BPHYT
_RS09815
+1.9
1.8
malate synthase
compare
BPHYT
_RS04290
+1.9
0.9
glycosyl transferase family 2
compare
BPHYT
_RS06985
+1.9
12.9
fumarate hydratase
compare
BPHYT
_RS06075
+1.9
14.3
hypothetical protein
compare
BPHYT
_RS25770
+1.9
1.4
hypothetical protein
compare
BPHYT
_RS14395
+1.9
8.6
sugar dehydratase
compare
BPHYT
_RS17100
+1.8
6.2
RNA pyrophosphohydrolase
compare
BPHYT
_RS04370
+1.8
0.6
glycosyl transferase
compare
BPHYT
_RS05155
+1.8
9.5
acetyltransferase GCN5
compare
BPHYT
_RS04285
+1.8
0.8
rhamnosyltransferase
compare
BPHYT
_RS05615
+1.8
1.3
hypothetical protein
compare
BPHYT
_RS03725
+1.8
1.1
serine hydroxymethyltransferase
compare
BPHYT
_RS17640
+1.8
1.8
2-alkenal reductase
compare
BPHYT
_RS16390
+1.8
6.1
MarR family transcriptional regulator
compare
BPHYT
_RS09660
+1.7
8.4
trehalose-6-phosphate synthase
compare
BPHYT
_RS02540
+1.7
4.0
hypothetical protein
compare
BPHYT
_RS12725
+1.7
14.6
regulator
compare
BPHYT
_RS05025
+1.7
1.4
sugar ABC transporter substrate-binding protein
compare
BPHYT
_RS07840
+1.7
1.8
muconate cycloisomerase
compare
BPHYT
_RS12840
+1.7
4.4
pyruvate dehydrogenase
compare
BPHYT
_RS00920
+1.6
4.8
transposase
compare
BPHYT
_RS25575
+1.6
1.9
alpha/beta hydrolase
compare
BPHYT
_RS23910
+1.6
6.5
phosphoribosyl-dephospho-CoA transferase
compare
BPHYT
_RS28065
+1.6
1.5
hypothetical protein
compare
BPHYT
_RS14845
+1.6
0.9
cysteine synthase
compare
BPHYT
_RS34485
+1.6
2.5
diguanylate phosphodiesterase
compare
BPHYT
_RS31060
+1.6
1.5
capsular polysaccharide biosynthesis protein
compare
BPHYT
_RS27370
+1.5
2.5
CoA transferase
compare
BPHYT
_RS31615
+1.5
1.3
thiamine pyrophosphate protein
compare
BPHYT
_RS25080
+1.5
2.0
glycine/betaine ABC transporter substrate-binding protein
compare
BPHYT
_RS33515
+1.5
8.7
porin
compare
BPHYT
_RS25470
+1.5
0.9
alkylhydroperoxidase
compare
BPHYT
_RS16840
+1.5
3.0
transketolase
compare
BPHYT
_RS06195
+1.5
1.8
membrane protein
compare
BPHYT
_RS04430
+1.5
2.2
amino acid ABC transporter ATPase
compare
BPHYT
_RS26695
+1.5
2.5
cobalamin biosynthesis protein CbiG
compare
BPHYT
_RS12150
+1.5
10.5
PII uridylyl-transferase
compare
BPHYT
_RS21475
+1.5
1.6
hypothetical protein
compare
BPHYT
_RS18910
+1.5
0.8
FlgN
compare
BPHYT
_RS31515
+1.5
2.4
agmatinase
compare
BPHYT
_RS22870
+1.5
1.5
copper transporter
compare
BPHYT
_RS14145
+1.5
7.1
phosphate ABC transporter substrate-binding protein
compare
BPHYT
_RS02005
+1.4
2.2
diguanylate phosphodiesterase
compare
BPHYT
_RS13200
+1.4
2.6
molybdopterin binding oxidoreductase
compare
BPHYT
_RS10345
+1.4
1.6
flagellar motor protein MotB
compare
BPHYT
_RS16060
+1.4
3.1
ribonucleotide-diphosphate reductase subunit alpha
compare
BPHYT
_RS34035
+1.4
2.0
type II and III secretion system outermembrane protein, secretin
compare
BPHYT
_RS02860
+1.4
0.7
membrane protein
compare
BPHYT
_RS36275
+1.4
1.0
hypothetical protein
compare
BPHYT
_RS09585
+1.4
0.8
hypothetical protein
compare
BPHYT
_RS16065
+1.4
0.9
LacI family transcriptional regulator
compare
BPHYT
_RS06975
+1.3
1.3
glutamate racemase
compare
BPHYT
_RS15620
+1.3
3.4
peptidyl-prolyl cis-trans isomerase
compare
BPHYT
_RS12985
+1.3
1.3
amino acid acetyltransferase
compare
BPHYT
_RS13080
+1.3
2.2
hypothetical protein
compare
BPHYT
_RS30995
+1.3
0.6
glucose-1-phosphate thymidylyltransferase
compare
BPHYT
_RS07255
+1.3
1.2
4-oxalocrotonate decarboxylase
compare
BPHYT
_RS05670
+1.3
2.1
hypothetical protein
compare
BPHYT
_RS28670
+1.3
1.4
MFS transporter
compare
BPHYT
_RS20095
+1.3
1.3
transcriptional regulator
compare
BPHYT
_RS20060
+1.3
1.5
hypothetical protein
compare
BPHYT
_RS08945
+1.3
5.0
hypothetical protein
compare
BPHYT
_RS11555
+1.3
2.3
allantoinase
compare
BPHYT
_RS34185
+1.3
4.1
cold-shock protein
compare
BPHYT
_RS09390
+1.3
2.2
ABC transporter permease
compare
BPHYT
_RS33030
+1.3
1.5
hypothetical protein
compare
BPHYT
_RS08995
+1.3
0.9
hypothetical protein
compare
BPHYT
_RS36585
+1.3
2.5
hypothetical protein
compare
BPHYT
_RS31225
+1.3
1.7
D-alanyl-D-alanine dipeptidase
compare
BPHYT
_RS02435
+1.3
1.3
hypothetical protein
compare
BPHYT
_RS30645
+1.3
2.3
hypothetical protein
compare
BPHYT
_RS04890
+1.3
1.3
cobalamin biosynthesis protein CobD
compare
BPHYT
_RS17290
+1.2
9.1
porin
compare
BPHYT
_RS11815
+1.2
1.3
alcohol dehydrogenase
compare
BPHYT
_RS11405
+1.2
3.0
hypothetical protein
compare
BPHYT
_RS34495
+1.2
2.3
phenol degradation protein meta
compare
BPHYT
_RS33215
+1.2
2.0
glycosyltransferase
compare
BPHYT
_RS20055
+1.2
1.7
hypothetical protein
compare
BPHYT
_RS28350
+1.2
1.7
fatty acid desaturase
compare
BPHYT
_RS02525
+1.2
7.4
membrane protein
compare
BPHYT
_RS05320
+1.2
1.4
beta-lactamase
compare
BPHYT
_RS26685
+1.2
0.9
precorrin-8X methylmutase
compare
BPHYT
_RS12280
+1.2
1.3
RNA helicase
compare
BPHYT
_RS07055
+1.2
2.9
sulfotransferase
compare
BPHYT
_RS14135
+1.2
5.1
phosphate transporter permease subunit PtsA
compare
BPHYT
_RS15320
+1.2
1.7
hypothetical protein
compare
BPHYT
_RS26975
+1.2
1.3
L-lactate dehydrogenase, LldE subunit
(from data)
compare
BPHYT
_RS06375
+1.2
2.6
ribonuclease
compare
BPHYT
_RS24135
+1.2
1.6
alpha-dehydro-beta-deoxy-D-glucarate aldolase
compare
BPHYT
_RS11005
+1.1
2.3
chromate transporter
compare
BPHYT
_RS25970
+1.1
0.7
urea amidolyase
compare
BPHYT
_RS23410
+1.1
2.6
hypothetical protein
compare
BPHYT
_RS07540
+1.1
0.9
hypothetical protein
compare
BPHYT
_RS08510
+1.1
1.3
acyl-CoA dehydrogenase
compare
BPHYT
_RS03460
+1.1
6.7
mammalian cell entry protein
compare
BPHYT
_RS20155
+1.1
1.0
hypothetical protein
compare
BPHYT
_RS28505
+1.1
0.9
5-deoxyglucuronate isomerase
compare
BPHYT
_RS13645
+1.1
9.0
threonine--tRNA ligase
compare
BPHYT
_RS11150
+1.1
0.9
LysR family transcriptional regulator
compare
BPHYT
_RS11895
+1.1
2.6
peroxiredoxin
compare
BPHYT
_RS17545
+1.1
4.3
cold-shock protein
compare
BPHYT
_RS10350
+1.1
2.3
hypothetical protein
compare
BPHYT
_RS36365
+1.1
1.1
hypothetical protein
compare
BPHYT
_RS09145
+1.1
7.1
poly-beta-hydroxybutyrate polymerase
compare
BPHYT
_RS10145
+1.1
2.8
Mn-containing catalase
compare
BPHYT
_RS25980
+1.1
0.9
urea carboxylase
compare
BPHYT
_RS15170
+1.1
3.3
family 2 glycosyl transferase
compare
BPHYT
_RS16345
+1.1
3.1
succinyl-CoA ligase [ADP-forming] subunit beta
compare
BPHYT
_RS09620
+1.1
1.1
aminodeoxychorismate lyase
compare
BPHYT
_RS26675
+1.1
1.7
precorrin-6Y C5,15-methyltransferase
compare
BPHYT
_RS24095
+1.1
2.2
membrane protein
compare
BPHYT
_RS02695
+1.1
4.8
photosynthetic protein synthase I
compare
BPHYT
_RS11535
+1.1
2.0
membrane protein
compare
BPHYT
_RS12590
+1.1
1.3
dual-specificity RNA methyltransferase RlmN
compare
BPHYT
_RS21055
+1.1
1.6
S-formylglutathione hydrolase
compare
BPHYT
_RS04315
+1.1
4.7
UDP-N-acetylglucosamine 2-epimerase
compare
BPHYT
_RS03090
+1.1
1.4
hypothetical protein
compare
BPHYT
_RS11960
+1.1
0.7
MarR family transcriptional regulator
compare
BPHYT
_RS16570
+1.1
1.1
glutathione S-transferase
compare
BPHYT
_RS16340
+1.1
3.8
succinyl-CoA synthetase subunit alpha
compare
BPHYT
_RS29365
+1.1
1.4
ATP-NAD kinase
compare
BPHYT
_RS15105
+1.1
2.6
FAD-dependent oxidoreductase
compare
BPHYT
_RS05585
+1.1
3.8
hypothetical protein
compare
BPHYT
_RS15200
+1.1
3.4
hypothetical protein
compare
BPHYT
_RS03450
+1.1
5.7
paraquat-inducible protein A
compare
BPHYT
_RS09640
+1.1
5.4
DNAase
compare
BPHYT
_RS17075
+1.1
2.8
ABC transporter permease
compare
BPHYT
_RS13935
+1.1
0.9
mannosyltransferase
compare
BPHYT
_RS20830
+1.0
1.7
electron transfer flavoprotein subunit beta
compare
BPHYT
_RS23955
+1.0
2.5
LysR family transcriptional regulator
compare
BPHYT
_RS31685
+1.0
1.5
D-isomer specific 2-hydroxyacid dehydrogenase
compare
BPHYT
_RS24250
+1.0
3.6
GCN5 family acetyltransferase
compare
BPHYT
_RS20880
+1.0
1.2
polyketide cyclase
compare
BPHYT
_RS15160
+1.0
4.5
hypothetical protein
compare
BPHYT
_RS22820
+1.0
2.0
hydrolase
compare
BPHYT
_RS06065
+1.0
5.4
cytochrome C
compare
BPHYT
_RS07900
+1.0
1.8
hypothetical protein
compare
BPHYT
_RS30085
+1.0
1.2
hypothetical protein
compare
BPHYT
_RS35550
+1.0
1.3
nitrile hydratase
compare
BPHYT
_RS33695
+1.0
1.1
molybdenum ABC transporter ATPase
compare
BPHYT
_RS31040
+1.0
1.5
sugar transporter
compare
BPHYT
_RS28360
+1.0
0.9
carboxymuconolactone decarboxylase
compare
BPHYT
_RS02640
+1.0
7.4
cytochrome C oxidase
compare
BPHYT
_RS31400
+1.0
1.3
methyl-accepting chemotaxis protein
compare
BPHYT
_RS08125
+1.0
7.0
ATPase
compare
BPHYT
_RS15145
+1.0
2.3
3-oxoacyl-ACP synthase
compare
BPHYT
_RS03465
+1.0
3.6
hypothetical protein
compare
BPHYT
_RS35215
+1.0
1.3
dehydrogenase
compare
BPHYT
_RS33170
+1.0
2.0
acetyltransferase
compare
BPHYT
_RS06435
+1.0
1.4
hypothetical protein
compare
BPHYT
_RS16815
+1.0
2.1
ureidoglycolate hydrolase
compare
BPHYT
_RS06610
+1.0
1.0
triosephosphate isomerase
compare
BPHYT
_RS01965
+1.0
1.2
tRNA-Ala
compare
BPHYT
_RS16290
+1.0
1.3
hypothetical protein
compare
BPHYT
_RS10315
+1.0
2.5
MFS transporter
compare
BPHYT
_RS08190
+1.0
1.6
type II secretion system protein
compare
BPHYT
_RS31675
+1.0
2.1
hypothetical protein
compare
BPHYT
_RS24270
+1.0
0.8
C4-dicarboxylate ABC transporter substrate-binding protein
compare
BPHYT
_RS02530
+1.0
3.5
hypothetical protein
compare
BPHYT
_RS35165
+1.0
1.5
peptidase
compare
BPHYT
_RS04340
+1.0
1.6
hypothetical protein
compare
BPHYT
_RS16210
+1.0
2.0
peptidase
compare
Specific Phenotypes
None in this experiment
For Burkholderia phytofirmans PsJN in phage experiments