Experiment set39S359 for Burkholderia phytofirmans PsJN

36hr PHYTO10 MOI 10

Group: phage
Media: LB_plus_SM_buffer + Kan (50 ug/ml)
Culturing: BFirm_ML3b, 48 well microplate; Epoch2, Aerobic, at 30 (C), shaken=double orbital, continuous, 205cpm
By: Marissa on 7/7/24
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 7 genes in this experiment

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• Benzoate degradation via CoA ligation
• Limonene and pinene degradation
• Biosynthesis of unsaturated fatty acids
• Ubiquinone and menaquinone biosynthesis
• Tyrosine metabolism
• Benzoate degradation via hydroxylation
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Nucleotide sugars metabolism
• alpha-Linolenic acid metabolism
• 1- and 2-Methylnaphthalene degradation
• Butanoate metabolism
• Porphyrin and chlorophyll metabolism
• Biosynthesis of type II polyketide backbone
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast)	12	12	3
gondoate biosynthesis (anaerobic)	4	4	1
fatty acid biosynthesis initiation (mitochondria)	4	2	1
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate)	9	9	2
oleate biosynthesis IV (anaerobic)	14	13	3
palmitate biosynthesis III	29	28	6
cis-vaccenate biosynthesis	5	5	1
fatty acid elongation -- saturated	5	5	1
superpathway of unsaturated fatty acids biosynthesis (E. coli)	20	19	4
tetradecanoate biosynthesis (mitochondria)	25	23	5
superpathway of fatty acid biosynthesis initiation	5	4	1
palmitate biosynthesis II (type II fatty acid synthase)	31	29	6
superpathway of fatty acids biosynthesis (E. coli)	53	50	10
superpathway of fatty acid biosynthesis I (E. coli)	16	14	3
8-amino-7-oxononanoate biosynthesis I	11	9	2
(5Z)-dodecenoate biosynthesis I	6	5	1
stearate biosynthesis II (bacteria and plants)	6	5	1
stearate biosynthesis IV	6	4	1
(5Z)-dodecenoate biosynthesis II	6	4	1
petroselinate biosynthesis	6	2	1
superpathway of fatty acid biosynthesis II (plant)	43	38	7
anteiso-branched-chain fatty acid biosynthesis	34	30	5
odd iso-branched-chain fatty acid biosynthesis	34	30	5
even iso-branched-chain fatty acid biosynthesis	34	30	5
biotin biosynthesis I	15	13	2
streptorubin B biosynthesis	34	20	4
mycolate biosynthesis	205	25	4
superpathway of mycolate biosynthesis	239	26	4