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Experiment set39S328 for
Burkholderia phytofirmans PsJN
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24hr PHYTO6 MOI 0.1
200 most detrimental genes:
gene
name
fitness
t score
description
BPHYT
_RS11705
+3.5
2.6
hypothetical protein
compare
BPHYT
_RS19570
+3.1
26.5
sodium/hydrogen exchanger
compare
BPHYT
_RS19565
+3.1
20.6
gamma-glutamyl:cysteine ligase
compare
BPHYT
_RS33820
+2.8
26.1
histidine kinase
compare
BPHYT
_RS14140
+2.7
5.8
phosphate transporter permease subunit PstC
compare
BPHYT
_RS14855
+2.3
1.1
ADP-L-glycero-D-manno-heptose-6-epimerase
compare
BPHYT
_RS02905
+2.3
20.4
RNA polymerase subunit sigma-54
compare
BPHYT
_RS25935
+2.2
1.7
type III secretion protein
compare
BPHYT
_RS04965
+2.2
12.9
ATP-binding protein
compare
BPHYT
_RS09330
+2.2
19.1
phytoene synthase
compare
BPHYT
_RS04945
+2.1
18.4
membrane protein
compare
BPHYT
_RS04950
+2.1
16.2
hypothetical protein
compare
BPHYT
_RS06075
+2.1
12.3
hypothetical protein
compare
BPHYT
_RS05575
+2.1
15.9
Fis family transcriptional regulator
compare
BPHYT
_RS06530
+2.0
2.3
membrane protein
compare
BPHYT
_RS12845
+2.0
15.5
ATPase
compare
BPHYT
_RS33920
+2.0
12.6
type II citrate synthase
compare
BPHYT
_RS05560
+2.0
13.9
Fis family transcriptional regulator
compare
BPHYT
_RS13645
+1.9
16.5
threonine--tRNA ligase
compare
BPHYT
_RS23910
+1.9
4.1
phosphoribosyl-dephospho-CoA transferase
compare
BPHYT
_RS14400
+1.9
4.7
anti-sigma factor
compare
BPHYT
_RS12725
+1.8
15.9
regulator
compare
BPHYT
_RS02595
+1.8
2.7
sulfurtransferase
compare
BPHYT
_RS09500
+1.8
9.1
glucose-6-phosphate isomerase
compare
BPHYT
_RS05590
+1.8
14.7
trehalose-6-phosphate synthase
compare
BPHYT
_RS18655
+1.7
7.5
3-oxoacyl-ACP synthase
compare
BPHYT
_RS06985
+1.7
9.7
fumarate hydratase
compare
BPHYT
_RS08850
+1.7
0.9
transcriptional regulator
compare
BPHYT
_RS04290
+1.7
1.1
glycosyl transferase family 2
compare
BPHYT
_RS28470
+1.6
1.8
hypothetical protein
compare
BPHYT
_RS07840
+1.6
3.0
muconate cycloisomerase
compare
BPHYT
_RS02540
+1.6
4.0
hypothetical protein
compare
BPHYT
_RS26975
+1.6
2.1
L-lactate dehydrogenase, LldE subunit
(from data)
compare
BPHYT
_RS16335
+1.5
4.3
membrane protein
compare
BPHYT
_RS05155
+1.5
8.3
acetyltransferase GCN5
compare
BPHYT
_RS09660
+1.5
9.0
trehalose-6-phosphate synthase
compare
BPHYT
_RS06175
+1.4
3.9
LPS biosynthesis
compare
BPHYT
_RS05610
+1.4
8.6
DNA-cytosine methyltransferase
compare
BPHYT
_RS08945
+1.4
8.7
hypothetical protein
compare
BPHYT
_RS16675
+1.4
1.6
dihydroorotase
compare
BPHYT
_RS02525
+1.4
7.7
membrane protein
compare
BPHYT
_RS12465
+1.4
3.1
inositol monophosphatase
compare
BPHYT
_RS04940
+1.4
3.0
hypothetical protein
compare
BPHYT
_RS27890
+1.4
0.7
hypothetical protein
compare
BPHYT
_RS27175
+1.4
1.7
DNA-binding protein
compare
BPHYT
_RS13200
+1.4
3.7
molybdopterin binding oxidoreductase
compare
BPHYT
_RS14145
+1.4
7.1
phosphate ABC transporter substrate-binding protein
compare
BPHYT
_RS17035
+1.4
1.6
sugar kinase
compare
BPHYT
_RS07960
+1.4
1.7
hypothetical protein
compare
BPHYT
_RS03455
+1.4
1.9
paraquat-inducible protein A
compare
BPHYT
_RS14395
+1.4
5.7
sugar dehydratase
compare
BPHYT
_RS12150
+1.4
10.2
PII uridylyl-transferase
compare
BPHYT
_RS16390
+1.3
4.7
MarR family transcriptional regulator
compare
BPHYT
_RS34295
+1.3
1.6
fimbrial protein
compare
BPHYT
_RS09815
+1.3
1.9
malate synthase
compare
BPHYT
_RS26080
+1.3
1.0
ABC transporter ATPase
compare
BPHYT
_RS28920
+1.3
1.6
cellulose synthase
compare
BPHYT
_RS32605
+1.3
1.9
hypothetical protein
compare
BPHYT
_RS14485
+1.3
5.2
ribonuclease E
compare
BPHYT
_RS29855
+1.3
1.4
protein-tyrosine-phosphatase
compare
BPHYT
_RS05615
+1.3
1.2
hypothetical protein
compare
BPHYT
_RS02640
+1.3
8.6
cytochrome C oxidase
compare
BPHYT
_RS01170
+1.2
2.1
hypothetical protein
compare
BPHYT
_RS25820
+1.2
2.1
sugar ABC transporter
compare
BPHYT
_RS26955
+1.2
1.7
succinylglutamate desuccinylase
compare
BPHYT
_RS06065
+1.2
6.3
cytochrome C
compare
BPHYT
_RS02650
+1.2
3.2
membrane protein
compare
BPHYT
_RS31615
+1.2
1.4
thiamine pyrophosphate protein
compare
BPHYT
_RS21460
+1.2
1.9
UDP-N-acetylglucosamine 2-epimerase
compare
BPHYT
_RS20725
+1.2
1.9
histidine kinase
compare
BPHYT
_RS05670
+1.2
2.3
hypothetical protein
compare
BPHYT
_RS30995
+1.2
0.5
glucose-1-phosphate thymidylyltransferase
compare
BPHYT
_RS06390
+1.1
2.1
ribosome-associated protein IOJAP
compare
BPHYT
_RS21495
+1.1
1.5
ABC transporter
compare
BPHYT
_RS07255
+1.1
2.3
4-oxalocrotonate decarboxylase
compare
BPHYT
_RS23540
+1.1
1.7
RND transporter
compare
BPHYT
_RS05705
+1.1
1.2
hypothetical protein
compare
BPHYT
_RS24500
+1.1
1.2
histidine kinase
compare
BPHYT
_RS06610
+1.1
1.0
triosephosphate isomerase
compare
BPHYT
_RS28670
+1.1
1.6
MFS transporter
compare
BPHYT
_RS28595
+1.1
1.2
glyoxalase
compare
BPHYT
_RS34185
+1.1
2.2
cold-shock protein
compare
BPHYT
_RS03620
+1.1
6.5
poly(A) polymerase
compare
BPHYT
_RS26675
+1.1
1.9
precorrin-6Y C5,15-methyltransferase
compare
BPHYT
_RS02635
+1.1
6.2
membrane protein
compare
BPHYT
_RS16840
+1.1
2.2
transketolase
compare
BPHYT
_RS05335
+1.1
2.6
AraC family transcriptional regulator
compare
BPHYT
_RS02865
+1.1
0.6
hypothetical protein
compare
BPHYT
_RS12635
+1.1
1.9
translation factor Sua5
compare
BPHYT
_RS02975
+1.1
1.3
membrane protein
compare
BPHYT
_RS10040
+1.1
1.2
hypothetical protein
compare
BPHYT
_RS22815
+1.0
1.8
TonB-denpendent receptor
compare
BPHYT
_RS16345
+1.0
2.9
succinyl-CoA ligase [ADP-forming] subunit beta
compare
BPHYT
_RS09640
+1.0
4.5
DNAase
compare
BPHYT
_RS04430
+1.0
1.5
amino acid ABC transporter ATPase
compare
BPHYT
_RS29250
+1.0
2.6
dihydrodipicolinate synthase
compare
BPHYT
_RS25645
+1.0
1.4
dehydrogenase
compare
BPHYT
_RS16430
+1.0
8.9
cytochrome C
compare
BPHYT
_RS12290
+1.0
1.6
universal stress protein UspA
compare
BPHYT
_RS26910
+1.0
2.0
hypothetical protein
compare
BPHYT
_RS16290
+1.0
1.5
hypothetical protein
compare
BPHYT
_RS04285
+1.0
0.5
rhamnosyltransferase
compare
BPHYT
_RS21475
+1.0
1.2
hypothetical protein
compare
BPHYT
_RS28410
+1.0
2.7
ubiquinol oxidase subunit II, cyanide insensitive
compare
BPHYT
_RS03625
+1.0
5.3
Histidinol-phosphatase (EC:3.1.3.15)
(from data)
compare
BPHYT
_RS14130
+1.0
3.1
phosphate ABC transporter ATP-binding protein
compare
BPHYT
_RS16435
+1.0
7.8
alcohol dehydrogenase
compare
BPHYT
_RS06070
+1.0
6.3
cytochrome C
compare
BPHYT
_RS15865
+1.0
1.8
exodeoxyribonuclease VII large subunit
compare
BPHYT
_RS13835
+1.0
1.4
S-adenosylhomocysteine nucleosidase
compare
BPHYT
_RS26700
+1.0
3.4
hypothetical protein
compare
BPHYT
_RS17095
+1.0
2.5
proline--tRNA ligase
compare
BPHYT
_RS09160
+1.0
3.8
polyhydroxyalkanoate synthesis repressor PhaR
compare
BPHYT
_RS07705
+1.0
1.8
arginine succinyltransferase
compare
BPHYT
_RS25490
+1.0
2.3
hypothetical protein
compare
BPHYT
_RS07055
+1.0
1.7
sulfotransferase
compare
BPHYT
_RS18255
+1.0
2.2
phenylacetate-CoA oxygenase subunit PaaB
compare
BPHYT
_RS03410
+1.0
1.8
queuine tRNA-ribosyltransferase
compare
BPHYT
_RS17945
+1.0
2.4
cytochrome C biogenesis protein
compare
BPHYT
_RS31685
+1.0
1.3
D-isomer specific 2-hydroxyacid dehydrogenase
compare
BPHYT
_RS15290
+1.0
2.2
tRNA-Arg
compare
BPHYT
_RS23940
+0.9
0.5
GntR family transcriptional regulator
compare
BPHYT
_RS31205
+0.9
1.5
peptide ABC transporter ATP-binding protein
compare
BPHYT
_RS19325
+0.9
2.4
glycine cleavage system H protein
compare
BPHYT
_RS15335
+0.9
5.7
coproporphyrinogen III oxidase
compare
BPHYT
_RS01455
+0.9
1.0
peptide deformylase
compare
BPHYT
_RS07060
+0.9
2.2
aldo/keto reductase
compare
BPHYT
_RS26390
+0.9
0.8
antibiotic resistance protein
compare
BPHYT
_RS09980
+0.9
0.9
TetR family transcriptional regulator
compare
BPHYT
_RS14135
+0.9
3.2
phosphate transporter permease subunit PtsA
compare
BPHYT
_RS08985
+0.9
3.9
Rrf2 family transcriptional regulator
compare
BPHYT
_RS13890
+0.9
2.4
RpiR family transcriptional regulator
compare
BPHYT
_RS06975
+0.9
0.8
glutamate racemase
compare
BPHYT
_RS01340
+0.9
1.1
ChrB domain-containing protein
compare
BPHYT
_RS27390
+0.9
2.4
hydroxyacid dehydrogenase
compare
BPHYT
_RS09145
+0.9
6.1
poly-beta-hydroxybutyrate polymerase
compare
BPHYT
_RS16175
+0.9
0.5
hemolysin D
compare
BPHYT
_RS13110
+0.9
2.7
ABC transporter substrate-binding protein
compare
BPHYT
_RS10320
+0.9
2.2
hypothetical protein
compare
BPHYT
_RS32335
+0.9
1.6
AsnC family transcriptional regulator
compare
BPHYT
_RS02665
+0.9
5.9
MFS transporter
compare
BPHYT
_RS16060
+0.9
2.1
ribonucleotide-diphosphate reductase subunit alpha
compare
BPHYT
_RS34025
+0.9
1.1
hypothetical protein
compare
BPHYT
_RS02435
+0.9
0.9
hypothetical protein
compare
BPHYT
_RS23035
+0.9
1.7
hypothetical protein
compare
BPHYT
_RS28575
+0.9
0.8
transcriptional regulator
compare
BPHYT
_RS15415
+0.9
5.5
3-octaprenyl-4-hydroxybenzoate carboxy-lyase
compare
BPHYT
_RS04015
+0.9
1.8
glycosyl transferase
compare
BPHYT
_RS22055
+0.9
2.3
DNA-binding protein
compare
BPHYT
_RS15620
+0.9
2.2
peptidyl-prolyl cis-trans isomerase
compare
BPHYT
_RS34485
+0.9
1.8
diguanylate phosphodiesterase
compare
BPHYT
_RS06350
+0.9
1.0
chemotaxis protein CheY
compare
BPHYT
_RS22835
+0.9
0.6
flagellar motor protein MotA
compare
BPHYT
_RS09695
+0.9
1.5
hypothetical protein
compare
BPHYT
_RS23975
+0.9
1.2
peroxidase
compare
BPHYT
_RS12990
+0.9
1.7
iron transporter
compare
BPHYT
_RS17545
+0.9
3.5
cold-shock protein
compare
BPHYT
_RS26930
+0.9
3.9
mechanosensitive ion channel protein
compare
BPHYT
_RS04625
+0.9
2.0
urea ABC transporter ATP-binding protein
compare
BPHYT
_RS34495
+0.9
1.9
phenol degradation protein meta
compare
BPHYT
_RS07300
+0.9
3.8
porin
compare
BPHYT
_RS03900
+0.9
1.3
phospholipid-binding protein
compare
BPHYT
_RS25020
+0.9
1.5
phospholipid-binding protein
compare
BPHYT
_RS35265
+0.9
3.9
LysR family transcriptional regulator
compare
BPHYT
_RS12640
+0.9
4.9
phosphoesterase
compare
BPHYT
_RS07145
+0.9
1.2
DNA-binding protein
compare
BPHYT
_RS34280
+0.9
1.4
hypothetical protein
compare
BPHYT
_RS10315
+0.9
2.0
MFS transporter
compare
BPHYT
_RS23895
+0.9
1.4
hypothetical protein
compare
BPHYT
_RS25575
+0.9
0.9
alpha/beta hydrolase
compare
BPHYT
_RS08125
+0.8
4.6
ATPase
compare
BPHYT
_RS04005
+0.8
1.5
GDP-L-fucose synthase
compare
BPHYT
_RS15305
+0.8
3.3
guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
compare
BPHYT
_RS08510
+0.8
1.2
acyl-CoA dehydrogenase
compare
BPHYT
_RS10145
+0.8
2.6
Mn-containing catalase
compare
BPHYT
_RS22855
+0.8
1.7
aldehyde-activating protein
compare
BPHYT
_RS12815
+0.8
3.3
IclR family transcriptional regulator
compare
BPHYT
_RS28985
+0.8
1.9
hypothetical protein
compare
BPHYT
_RS04750
+0.8
2.2
uroporphyrin-III C-methyltransferase
compare
BPHYT
_RS07465
+0.8
2.1
FAD-dependent pyridine nucleotide-disulfide oxidoreductase
compare
BPHYT
_RS16210
+0.8
2.1
peptidase
compare
BPHYT
_RS34920
+0.8
1.1
IclR family transcriptional regulator
compare
BPHYT
_RS14340
+0.8
5.6
Fis family transcriptional regulator
compare
BPHYT
_RS15360
+0.8
1.4
hypothetical protein
compare
BPHYT
_RS23875
+0.8
0.9
sugar ABC transporter ATP-binding protein
compare
BPHYT
_RS25080
+0.8
0.9
glycine/betaine ABC transporter substrate-binding protein
compare
BPHYT
_RS14845
+0.8
0.5
cysteine synthase
compare
BPHYT
_RS28270
+0.8
2.5
ABC transporter substrate-binding protein
compare
BPHYT
_RS19915
+0.8
2.5
hemolysin D
compare
BPHYT
_RS23615
+0.8
2.1
XRE family transcriptional regulator
compare
BPHYT
_RS35115
+0.8
2.2
phospholipase C
compare
BPHYT
_RS08545
+0.8
0.8
oxidoreductase
compare
BPHYT
_RS14175
+0.8
2.1
RNA-binding protein
compare
BPHYT
_RS05395
+0.8
1.2
hypothetical protein
compare
BPHYT
_RS20220
+0.8
0.9
antibiotic synthesis protein MbtH
compare
BPHYT
_RS06605
+0.8
2.5
NAD(P)H quinone oxidoreductase
compare
BPHYT
_RS17725
+0.8
4.8
BolA family transcriptional regulator
compare
BPHYT
_RS32200
+0.8
1.3
lytic transglycosylase
compare
BPHYT
_RS31515
+0.8
1.7
agmatinase
compare
BPHYT
_RS15330
+0.8
4.2
nucleoside-triphosphate diphosphatase
compare
Specific Phenotypes
None in this experiment
For Burkholderia phytofirmans PsJN in phage experiments