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Experiment set39S304 for
Burkholderia phytofirmans PsJN
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24hr PHYTO2 MOI 0.1
200 most detrimental genes:
gene
name
fitness
t score
description
BPHYT
_RS19570
+3.2
28.6
sodium/hydrogen exchanger
compare
BPHYT
_RS19565
+3.1
20.1
gamma-glutamyl:cysteine ligase
compare
BPHYT
_RS11705
+2.9
2.4
hypothetical protein
compare
BPHYT
_RS33820
+2.7
25.6
histidine kinase
compare
BPHYT
_RS16710
+2.6
1.3
LysR family transcriptional regulator
compare
BPHYT
_RS02905
+2.3
20.2
RNA polymerase subunit sigma-54
compare
BPHYT
_RS02595
+2.2
3.0
sulfurtransferase
compare
BPHYT
_RS25935
+2.2
1.9
type III secretion protein
compare
BPHYT
_RS04945
+2.1
19.4
membrane protein
compare
BPHYT
_RS09330
+2.1
18.6
phytoene synthase
compare
BPHYT
_RS05575
+2.0
16.6
Fis family transcriptional regulator
compare
BPHYT
_RS04950
+2.0
16.8
hypothetical protein
compare
BPHYT
_RS05560
+2.0
11.5
Fis family transcriptional regulator
compare
BPHYT
_RS14140
+1.9
4.3
phosphate transporter permease subunit PstC
compare
BPHYT
_RS06075
+1.9
14.0
hypothetical protein
compare
BPHYT
_RS20155
+1.9
1.8
hypothetical protein
compare
BPHYT
_RS33920
+1.9
13.0
type II citrate synthase
compare
BPHYT
_RS12845
+1.9
15.3
ATPase
compare
BPHYT
_RS13645
+1.8
16.7
threonine--tRNA ligase
compare
BPHYT
_RS05590
+1.8
15.0
trehalose-6-phosphate synthase
compare
BPHYT
_RS12725
+1.8
15.9
regulator
compare
BPHYT
_RS04940
+1.8
3.0
hypothetical protein
compare
BPHYT
_RS14400
+1.8
4.4
anti-sigma factor
compare
BPHYT
_RS04290
+1.7
1.1
glycosyl transferase family 2
compare
BPHYT
_RS05155
+1.7
11.4
acetyltransferase GCN5
compare
BPHYT
_RS25340
+1.6
3.4
sulfatase
compare
BPHYT
_RS14395
+1.6
8.4
sugar dehydratase
compare
BPHYT
_RS04370
+1.6
0.5
glycosyl transferase
compare
BPHYT
_RS17905
+1.6
2.3
fimbrial assembly family protein
compare
BPHYT
_RS02435
+1.6
1.5
hypothetical protein
compare
BPHYT
_RS24095
+1.6
4.8
membrane protein
compare
BPHYT
_RS06530
+1.5
1.9
membrane protein
compare
BPHYT
_RS07960
+1.5
2.1
hypothetical protein
compare
BPHYT
_RS09500
+1.5
7.2
glucose-6-phosphate isomerase
compare
BPHYT
_RS09815
+1.5
3.2
malate synthase
compare
BPHYT
_RS10400
+1.5
1.6
taurine ABC transporter permease
compare
BPHYT
_RS26570
+1.5
1.7
5'-nucleotidase
compare
BPHYT
_RS16675
+1.5
1.8
dihydroorotase
compare
BPHYT
_RS23910
+1.5
3.9
phosphoribosyl-dephospho-CoA transferase
compare
BPHYT
_RS16335
+1.5
4.2
membrane protein
compare
BPHYT
_RS33400
+1.5
2.7
magnesium transporter CorA
compare
BPHYT
_RS05610
+1.5
9.6
DNA-cytosine methyltransferase
compare
BPHYT
_RS08945
+1.5
8.8
hypothetical protein
compare
BPHYT
_RS12150
+1.5
11.4
PII uridylyl-transferase
compare
BPHYT
_RS06985
+1.4
6.6
fumarate hydratase
compare
BPHYT
_RS18655
+1.4
7.2
3-oxoacyl-ACP synthase
compare
BPHYT
_RS09660
+1.4
8.2
trehalose-6-phosphate synthase
compare
BPHYT
_RS31615
+1.4
2.0
thiamine pyrophosphate protein
compare
BPHYT
_RS00690
+1.4
1.2
transcriptional regulator
compare
BPHYT
_RS02640
+1.4
10.5
cytochrome C oxidase
compare
BPHYT
_RS18600
+1.4
2.6
glutathione S-transferase
compare
BPHYT
_RS12465
+1.4
3.0
inositol monophosphatase
compare
BPHYT
_RS28930
+1.4
1.8
chromosome partitioning protein ParA
compare
BPHYT
_RS02865
+1.3
1.1
hypothetical protein
compare
BPHYT
_RS06175
+1.3
3.2
LPS biosynthesis
compare
BPHYT
_RS06990
+1.3
1.1
membrane protein
compare
BPHYT
_RS02525
+1.3
6.9
membrane protein
compare
BPHYT
_RS29175
+1.3
3.2
ABC transporter ATP-binding protein
compare
BPHYT
_RS27330
+1.3
1.9
hypothetical protein
compare
BPHYT
_RS16345
+1.3
3.4
succinyl-CoA ligase [ADP-forming] subunit beta
compare
BPHYT
_RS20665
+1.3
0.9
LysR family transcriptional regulator
compare
BPHYT
_RS20790
+1.3
1.2
TetR family transcriptional regulator
compare
BPHYT
_RS17240
+1.3
0.7
cell division protein FtsW
compare
BPHYT
_RS16390
+1.3
4.4
MarR family transcriptional regulator
compare
BPHYT
_RS01630
+1.3
1.2
ATP-dependent protease ATPase subunit HslU
compare
BPHYT
_RS03620
+1.3
7.1
poly(A) polymerase
compare
BPHYT
_RS35310
+1.3
1.6
GCN5 family N-acetyltransferase
compare
BPHYT
_RS18635
+1.3
3.3
LamB/YcsF family protein
compare
BPHYT
_RS08850
+1.3
0.6
transcriptional regulator
compare
BPHYT
_RS08565
+1.3
0.7
dihydroorotate dehydrogenase
compare
BPHYT
_RS02650
+1.2
3.3
membrane protein
compare
BPHYT
_RS14855
+1.2
0.5
ADP-L-glycero-D-manno-heptose-6-epimerase
compare
BPHYT
_RS07840
+1.2
1.6
muconate cycloisomerase
compare
BPHYT
_RS12990
+1.2
3.0
iron transporter
compare
BPHYT
_RS14145
+1.2
6.5
phosphate ABC transporter substrate-binding protein
compare
BPHYT
_RS14135
+1.2
4.9
phosphate transporter permease subunit PtsA
compare
BPHYT
_RS30085
+1.2
1.7
hypothetical protein
compare
BPHYT
_RS12290
+1.2
2.4
universal stress protein UspA
compare
BPHYT
_RS17445
+1.2
3.2
GTP-binding protein YchF
compare
BPHYT
_RS07860
+1.2
2.3
benzoate 1,2-dioxygenase subunit beta
compare
BPHYT
_RS31550
+1.2
2.0
hypothetical protein
compare
BPHYT
_RS07570
+1.1
1.2
hypothetical protein
compare
BPHYT
_RS10145
+1.1
2.8
Mn-containing catalase
compare
BPHYT
_RS23540
+1.1
3.0
RND transporter
compare
BPHYT
_RS10995
+1.1
2.1
GntR family transcriptional regulator
compare
BPHYT
_RS34185
+1.1
3.1
cold-shock protein
compare
BPHYT
_RS13610
+1.1
1.7
MerR family transcriptional regulator
compare
BPHYT
_RS27655
+1.1
1.3
potassium transporter
compare
BPHYT
_RS06065
+1.1
6.3
cytochrome C
compare
BPHYT
_RS33150
+1.1
1.2
beta-lactamase
compare
BPHYT
_RS34745
+1.1
1.1
hypothetical protein
compare
BPHYT
_RS14845
+1.1
0.9
cysteine synthase
compare
BPHYT
_RS17885
+1.1
3.0
3-dehydroquinate synthase
compare
BPHYT
_RS26955
+1.1
1.5
succinylglutamate desuccinylase
compare
BPHYT
_RS32960
+1.1
2.0
short-chain dehydrogenase
compare
BPHYT
_RS06105
+1.1
1.2
hypothetical protein
compare
BPHYT
_RS24135
+1.1
2.6
alpha-dehydro-beta-deoxy-D-glucarate aldolase
compare
BPHYT
_RS00295
+1.1
1.8
hypothetical protein
compare
BPHYT
_RS12840
+1.1
2.5
pyruvate dehydrogenase
compare
BPHYT
_RS01170
+1.1
1.2
hypothetical protein
compare
BPHYT
_RS30865
+1.1
1.6
sugar ABC transporter ATP-binding protein
compare
BPHYT
_RS02635
+1.1
5.8
membrane protein
compare
BPHYT
_RS03625
+1.0
4.8
Histidinol-phosphatase (EC:3.1.3.15)
(from data)
compare
BPHYT
_RS31395
+1.0
3.8
thiamine pyrophosphate-binding protein
compare
BPHYT
_RS29520
+1.0
0.8
hypothetical protein
compare
BPHYT
_RS33390
+1.0
2.0
phosphate ABC transporter substrate-binding protein
compare
BPHYT
_RS07255
+1.0
1.8
4-oxalocrotonate decarboxylase
compare
BPHYT
_RS33695
+1.0
1.7
molybdenum ABC transporter ATPase
compare
BPHYT
_RS06400
+1.0
3.3
oxygen-dependent coproporphyrinogen-III oxidase
compare
BPHYT
_RS10320
+1.0
2.0
hypothetical protein
compare
BPHYT
_RS29720
+1.0
1.1
molybdopterin-guanine dinucleotide biosynthesis protein MobA
compare
BPHYT
_RS05320
+1.0
1.4
beta-lactamase
compare
BPHYT
_RS23155
+1.0
1.3
Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)
(from data)
compare
BPHYT
_RS04285
+1.0
0.5
rhamnosyltransferase
compare
BPHYT
_RS05670
+1.0
2.1
hypothetical protein
compare
BPHYT
_RS29710
+1.0
2.4
FAD-binding molybdopterin dehydrogenase
compare
BPHYT
_RS05585
+1.0
3.9
hypothetical protein
compare
BPHYT
_RS02530
+1.0
6.1
hypothetical protein
compare
BPHYT
_RS34125
+1.0
3.7
heavy metal translocating P-type ATPase
compare
BPHYT
_RS14340
+1.0
6.4
Fis family transcriptional regulator
compare
BPHYT
_RS13470
+1.0
0.7
arylsulfate sulfotransferase
compare
BPHYT
_RS11250
+1.0
1.4
saccharopine dehydrogenase
compare
BPHYT
_RS05290
+1.0
0.8
hypothetical protein
compare
BPHYT
_RS26775
+1.0
1.3
chemotaxis protein CheB
compare
BPHYT
_RS28075
+1.0
1.2
cytochrome B561
compare
BPHYT
_RS26780
+1.0
5.9
chemotaxis protein CheR
compare
BPHYT
_RS22045
+1.0
1.8
peptide ABC transporter permease
compare
BPHYT
_RS17265
+1.0
1.2
cell division protein
compare
BPHYT
_RS28920
+1.0
1.1
cellulose synthase
compare
BPHYT
_RS16060
+1.0
2.1
ribonucleotide-diphosphate reductase subunit alpha
compare
BPHYT
_RS05615
+1.0
0.8
hypothetical protein
compare
BPHYT
_RS03400
+1.0
0.5
ATP-dependent DNA helicase RecG
compare
BPHYT
_RS30425
+1.0
1.6
LysR family transcriptional regulator
compare
BPHYT
_RS27060
+1.0
1.3
photosystem reaction center subunit H
compare
BPHYT
_RS34035
+0.9
1.6
type II and III secretion system outermembrane protein, secretin
compare
BPHYT
_RS06605
+0.9
3.4
NAD(P)H quinone oxidoreductase
compare
BPHYT
_RS28505
+0.9
1.1
5-deoxyglucuronate isomerase
compare
BPHYT
_RS30720
+0.9
0.9
hypothetical protein
compare
BPHYT
_RS17945
+0.9
2.4
cytochrome C biogenesis protein
compare
BPHYT
_RS16430
+0.9
8.1
cytochrome C
compare
BPHYT
_RS25065
+0.9
1.4
glycine/betaine ABC transporter ATPase
compare
BPHYT
_RS17545
+0.9
3.9
cold-shock protein
compare
BPHYT
_RS02540
+0.9
2.1
hypothetical protein
compare
BPHYT
_RS02685
+0.9
5.8
cytochrome C oxidase subunit I
compare
BPHYT
_RS10175
+0.9
1.8
membrane protein
compare
BPHYT
_RS10230
+0.9
1.3
hypothetical protein
compare
BPHYT
_RS29250
+0.9
2.2
dihydrodipicolinate synthase
compare
BPHYT
_RS28595
+0.9
0.9
glyoxalase
compare
BPHYT
_RS32415
+0.9
3.0
translation elongation factor
compare
BPHYT
_RS03170
+0.9
0.9
membrane protein
compare
BPHYT
_RS32435
+0.9
2.4
formate dehydrogenase-N subunit beta
compare
BPHYT
_RS20220
+0.9
0.8
antibiotic synthesis protein MbtH
compare
BPHYT
_RS09640
+0.9
3.9
DNAase
compare
BPHYT
_RS08665
+0.9
0.7
hypothetical protein
compare
BPHYT
_RS34485
+0.9
2.2
diguanylate phosphodiesterase
compare
BPHYT
_RS02880
+0.9
1.0
ATP-dependent protease
compare
BPHYT
_RS24640
+0.9
2.3
alcohol dehydrogenase
compare
BPHYT
_RS04720
+0.9
5.4
transcriptional regulator
compare
BPHYT
_RS22485
+0.9
1.9
NADP-dependent aryl-alcohol dehydrogenase
compare
BPHYT
_RS07705
+0.9
2.0
arginine succinyltransferase
compare
BPHYT
_RS11965
+0.9
2.8
alkylhydroperoxidase
compare
BPHYT
_RS25320
+0.9
3.1
cobalamin biosynthesis protein CobW
compare
BPHYT
_RS06070
+0.9
5.3
cytochrome C
compare
BPHYT
_RS26675
+0.9
1.5
precorrin-6Y C5,15-methyltransferase
compare
BPHYT
_RS07325
+0.9
0.4
GntR family transcriptional regulator
compare
BPHYT
_RS16435
+0.9
6.8
alcohol dehydrogenase
compare
BPHYT
_RS26015
+0.9
1.2
rod shape-determining protein MreB
compare
BPHYT
_RS14485
+0.9
2.9
ribonuclease E
compare
BPHYT
_RS04385
+0.9
1.7
nucleoside-diphosphate sugar oxidoreductase
compare
BPHYT
_RS21700
+0.9
1.4
phosphotransacetylase
compare
BPHYT
_RS06725
+0.9
2.7
MaoC family dehydratase
compare
BPHYT
_RS24270
+0.9
0.8
C4-dicarboxylate ABC transporter substrate-binding protein
compare
BPHYT
_RS35100
+0.9
1.9
ABC transporter substrate-binding protein
compare
BPHYT
_RS24550
+0.9
1.7
hypothetical protein
compare
BPHYT
_RS30210
+0.9
2.4
GCN5 family acetyltransferase
compare
BPHYT
_RS01620
+0.9
5.3
sensor histidine kinase
compare
BPHYT
_RS09445
+0.9
2.0
trigger factor
compare
BPHYT
_RS02665
+0.9
5.5
MFS transporter
compare
BPHYT
_RS08510
+0.9
1.6
acyl-CoA dehydrogenase
compare
BPHYT
_RS23975
+0.9
1.1
peroxidase
compare
BPHYT
_RS28285
+0.9
1.6
MFS transporter
compare
BPHYT
_RS29410
+0.9
3.5
HPr kinase
compare
BPHYT
_RS25575
+0.9
0.9
alpha/beta hydrolase
compare
BPHYT
_RS17035
+0.9
0.7
sugar kinase
compare
BPHYT
_RS01505
+0.9
2.5
7-cyano-7-deazaguanine reductase
compare
BPHYT
_RS28200
+0.9
0.9
sugar ABC transporter substrate-binding protein
compare
BPHYT
_RS09395
+0.9
3.3
ABC transporter permease
compare
BPHYT
_RS09145
+0.9
5.6
poly-beta-hydroxybutyrate polymerase
compare
BPHYT
_RS16175
+0.9
0.5
hemolysin D
compare
BPHYT
_RS23445
+0.9
0.9
hexuronate transporter
compare
BPHYT
_RS23875
+0.8
0.9
sugar ABC transporter ATP-binding protein
compare
BPHYT
_RS34445
+0.8
2.3
GntR family transcriptional regulator
compare
BPHYT
_RS06375
+0.8
1.9
ribonuclease
compare
BPHYT
_RS17030
+0.8
1.0
lipid hydroperoxide peroxidase
compare
BPHYT
_RS13715
+0.8
3.4
hypothetical protein
compare
BPHYT
_RS33740
+0.8
1.8
hypothetical protein
compare
BPHYT
_RS15620
+0.8
2.1
peptidyl-prolyl cis-trans isomerase
compare
BPHYT
_RS23125
+0.8
3.0
FAD-dependent oxidoreductase
compare
BPHYT
_RS24045
+0.8
2.5
amino acid ABC transporter
compare
BPHYT
_RS15335
+0.8
5.5
coproporphyrinogen III oxidase
compare
Specific Phenotypes
None in this experiment
For Burkholderia phytofirmans PsJN in phage experiments