Experiment set36IT014 for Klebsiella michiganensis M5al

K. M5a1 KLEB22 MOI 1

Group: phage
Media: LB_plus_SM_buffer + Kan (50 ug/ml)
Culturing: Koxy_ML2a, 48 well microplate; Epoch2, Aerobic, at 30 (C), shaken=double orbital, continuous, 205cpm
By: Vivek on 3/10/23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 8 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Colanic acid biosynthesis	1
D-ribose utilization	1
Deoxyribose and Deoxynucleoside Catabolism	1
Sucrose utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• T cell receptor signaling pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
ribose phosphorylation	2	2	1
2-deoxy-D-ribose degradation I	3	3	1
Salmonella enterica serotype O:3,10 O antigen biosynthesis	5	2	1
Porphyromonas gingivalis O-LPS antigen biosynthesis	6	2	1
Salmonella enterica serotype O:4 O antigen biosynthesis (group B1)	6	2	1
Salmonella enterica serotype O:9 O antigen biosynthesis	6	2	1
Salmonella enterica serotype O:2 O antigen biosynthesis	6	2	1
Salmonella enterica serotype O:9,46 O antigen biosynthesis	6	2	1
Salmonella enterica serotype O:8 O antigen biosynthesis	8	1	1
Salmonella enterica serotype O:9,46,27 O antigen biosynthesis	9	2	1
succinoglycan biosynthesis	14	1	1