Experiment set36IT009 for Klebsiella michiganensis M5al

K. M5a1 KLEB23 MOI 1

Group: phage
Media: LB_plus_SM_buffer + Kan (50 ug/ml)
Culturing: Koxy_ML2a, 48 well microplate; Epoch2, Aerobic, at 30 (C), shaken=double orbital, continuous, 205cpm
By: Vivek on 3/10/23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 14 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Chorismate Synthesis	1
Colanic acid biosynthesis	1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate)	1
D-ribose utilization	1
Deoxyribose and Deoxynucleoside Catabolism	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Nitrogen fixation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Glycerophospholipid metabolism
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones
• T cell receptor signaling pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycerophosphodiester degradation	2	2	1
ribose phosphorylation	2	2	1
phosphatidylcholine resynthesis via glycerophosphocholine	2	1	1
2-deoxy-D-ribose degradation I	3	3	1
glycerol and glycerophosphodiester degradation	4	4	1
phospholipid remodeling (phosphatidylethanolamine, yeast)	4	2	1
chorismate biosynthesis from 3-dehydroquinate	5	5	1
Salmonella enterica serotype O:3,10 O antigen biosynthesis	5	2	1
Salmonella enterica serotype O:2 O antigen biosynthesis	6	2	1
Porphyromonas gingivalis O-LPS antigen biosynthesis	6	2	1
Salmonella enterica serotype O:9,46 O antigen biosynthesis	6	2	1
Salmonella enterica serotype O:4 O antigen biosynthesis (group B1)	6	2	1
Salmonella enterica serotype O:9 O antigen biosynthesis	6	2	1
chorismate biosynthesis I	7	7	1
Salmonella enterica serotype O:8 O antigen biosynthesis	8	1	1
Salmonella enterica serotype O:9,46,27 O antigen biosynthesis	9	2	1
superpathway of L-tyrosine biosynthesis	10	10	1
superpathway of L-phenylalanine biosynthesis	10	10	1
peptidoglycan recycling II	10	7	1
chorismate biosynthesis II (archaea)	12	8	1
anandamide biosynthesis I	12	3	1
superpathway of L-tryptophan biosynthesis	13	13	1
peptidoglycan recycling I	14	14	1
succinoglycan biosynthesis	14	1	1
superpathway of aromatic amino acid biosynthesis	18	18	1
superpathway of chorismate metabolism	59	54	1