Experiment set32S99 for Bacteroides thetaiotaomicron VPI-5482
B-glucan from yeast; autoclaved 2.5 mg/ml carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + B-glucan from yeast (2.5 mg/ml)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 8 genes in this experiment
For carbon source B-glucan from yeast in Bacteroides thetaiotaomicron VPI-5482
For carbon source B-glucan from yeast across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Restriction-Modification System | 4 |
| Aromatic amino acid degradation | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-tryptophan degradation II (via pyruvate) | 3 | 3 | 3 |
| L-serine degradation | 3 | 3 | 2 |
| L-cysteine degradation II | 3 | 2 | 2 |
| D-serine degradation | 3 | 2 | 2 |
| felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis | 5 | 2 | 2 |
| L-methionine biosynthesis II | 6 | 3 | 2 |
| glycine betaine degradation III | 7 | 4 | 2 |
| glycine betaine degradation I | 8 | 4 | 2 |
| L-mimosine degradation | 8 | 4 | 2 |
| glutathione-mediated detoxification I | 8 | 2 | 2 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 12 | 2 |
| purine nucleobases degradation II (anaerobic) | 24 | 12 | 2 |