Experiment set32S135 for Bacteroides thetaiotaomicron VPI-5482
L-Asparagine nitrogen source; Varel_Bryant_medium_Glucose_DTT_NaS_noNitrogen
Group: nitrogen sourceMedia: Varel_Bryant_medium_Glucose_DTT_NaS_noNitrogen + L-Asparagine (10 mM)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 3 mM Dithiothreitol, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, 3 mM Sodium sulfide nonahydrate, Mineral 3B solution minus Nitrogen (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 1 genes in this experiment
For nitrogen source L-Asparagine in Bacteroides thetaiotaomicron VPI-5482
For nitrogen source L-Asparagine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cysteine Biosynthesis | 1 |
| Methionine Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-cysteine biosynthesis I | 2 | 2 | 1 |
| seleno-amino acid biosynthesis (plants) | 5 | 2 | 1 |
| superpathway of sulfate assimilation and cysteine biosynthesis | 9 | 7 | 1 |
| superpathway of seleno-compound metabolism | 19 | 5 | 1 |