Experiment set32S110 for Bacteroides thetaiotaomicron VPI-5482

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Rhamnogalacturonan - from potato; autoclaved 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + Rhamnogalacturonan - from potato (2.5 mg/ml)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source Rhamnogalacturonan - from potato in Bacteroides thetaiotaomicron VPI-5482

For carbon source Rhamnogalacturonan - from potato across organisms

SEED Subsystems

Subsystem #Specific
Alginate metabolism 1
D-Galacturonate and D-Glucuronate Utilization 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.