Experiment set32S107 for Bacteroides thetaiotaomicron VPI-5482
Mannan from Saccharomyces cerevisiae; autoclaved 2.5 mg/ml carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + Mannan from Saccharomyces cerevisiae (2.5 mg/ml)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 6 genes in this experiment
For carbon source Mannan from Saccharomyces cerevisiae in Bacteroides thetaiotaomicron VPI-5482
For carbon source Mannan from Saccharomyces cerevisiae across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Archaeal lipids | 1 |
| Iron acquisition in Vibrio | 1 |
| Isoprenoid Biosynthesis | 1 |
| Proline Synthesis | 1 |
| Ton and Tol transport systems | 1 |
| polyprenyl synthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Arginine and proline metabolism
- Terpenoid biosynthesis
- Biosynthesis of terpenoids and steroids
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: