Experiment set32S100 for Bacteroides thetaiotaomicron VPI-5482

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B-glucan from yeast; autoclaved 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + B-glucan from yeast (2.5 mg/ml)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 6 genes in this experiment

For carbon source B-glucan from yeast in Bacteroides thetaiotaomicron VPI-5482

For carbon source B-glucan from yeast across organisms

SEED Subsystems

Subsystem #Specific
Restriction-Modification System 5

Metabolic Maps

Color code by fitness: see overview map or list of maps.