Experiment set32IT056 for Klebsiella michiganensis M5al

200 most detrimental genes:

gene	name	fitness	t score	description
BWI76_RS23140		+5.9	3.0	carboxysome shell protein
BWI76_RS15195		+4.6	2.2	DUF4186 domain-containing protein
BWI76_RS10735		+4.2	17.4	hypothetical protein
BWI76_RS06525		+3.8	27.0	ATP-dependent Clp protease proteolytic subunit
BWI76_RS06570		+3.4	1.5	thiamin pyrimidine pyrophosphate hydrolase
BWI76_RS06530		+3.3	10.0	ATP-dependent Clp protease ATP-binding subunit ClpX
BWI76_RS27780		+3.2	1.2	hypothetical protein
BWI76_RS17780		+3.0	2.2	septum formation inhibitor
BWI76_RS21165		+2.8	7.3	Fe-S cluster assembly transcriptional regulator IscR
BWI76_RS20430		+2.8	1.7	penicillin-insensitive murein endopeptidase
BWI76_RS17770		+2.8	2.1	isomerase/hydrolase
BWI76_RS11040		+2.7	1.4	ribonuclease E
BWI76_RS21945		+2.7	1.3	LysR family transcriptional regulator
BWI76_RS23365		+2.6	2.8	LysR family transcriptional regulator
BWI76_RS16900		+2.5	2.0	4-hydroxy-2-oxovalerate aldolase
BWI76_RS04260		+2.4	1.2	peroxide stress protein YaaA
BWI76_RS06745		+2.3	1.1	N-acetyltransferase GCN5
BWI76_RS14805		+2.2	1.3	hypothetical protein
BWI76_RS16930		+2.1	1.3	transcriptional regulator
BWI76_RS12815		+2.1	1.9	transcriptional regulator FeaR
BWI76_RS18820		+2.0	1.3	nitrogenase molybdenum-iron protein subunit beta
BWI76_RS16975		+2.0	1.6	cysteine desufuration protein SufE
BWI76_RS18175		+2.0	1.2	hydrolase
BWI76_RS18140		+1.9	1.3	zinc ABC transporter permease
BWI76_RS18135		+1.9	2.5	zinc ABC transporter ATP-binding protein ZnuC
BWI76_RS12495		+1.9	4.0	2-Cys peroxiredoxin
BWI76_RS15285		+1.9	1.4	stress protection protein MarC
BWI76_RS12605		+1.9	2.5	hypothetical protein
BWI76_RS08350		+1.9	2.4	carboxylase
BWI76_RS25000		+1.8	3.0	malonate decarboxylase acyl carrier protein
BWI76_RS10725		+1.8	1.8	hypothetical protein
BWI76_RS13155		+1.8	1.2	hypothetical protein
BWI76_RS26855		+1.6	1.5	cellulose synthase operon protein YhjQ
BWI76_RS15030		+1.6	0.9	ABC transporter
BWI76_RS10900		+1.6	1.6	putative oxidoreductase
BWI76_RS08670		+1.6	2.9	N-acetyltransferase
BWI76_RS07830		+1.6	0.8	3-phytase
BWI76_RS08600		+1.6	1.9	3-deoxy-7-phosphoheptulonate synthase
BWI76_RS15670		+1.6	1.2	hypothetical protein
BWI76_RS22715		+1.5	1.1	type I-E CRISPR-associated endoribonuclease Cas2
BWI76_RS13710		+1.5	1.4	benzoate 1,2-dioxygenase small subunit
BWI76_RS13720		+1.5	1.1	1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase
BWI76_RS18065		+1.5	3.7	DNA polymerase III subunit theta
BWI76_RS16490		+1.5	3.4	glutaredoxin
BWI76_RS00415		+1.5	2.0	glucose-1-phosphatase
BWI76_RS11350		+1.5	1.3	DeoR family transcriptional regulator
BWI76_RS16990		+1.5	1.8	ABC transporter ATP-binding protein
BWI76_RS18235		+1.4	1.2	trehalose-phosphatase
BWI76_RS22510		+1.4	5.5	hypothetical protein
BWI76_RS26875		+1.4	2.8	dipeptide ABC transporter permease DppC
BWI76_RS06630		+1.4	1.1	hypothetical protein
BWI76_RS00565		+1.4	4.4	branched-chain amino acid transport
BWI76_RS08625		+1.4	1.1	UDP-glucose 4-epimerase GalE
BWI76_RS23215		+1.4	1.3	N-acetylmuramoyl-L-alanine amidase
BWI76_RS00715		+1.4	2.7	two-component sensor histidine kinase
BWI76_RS15275		+1.4	1.1	isomerase
BWI76_RS17565		+1.4	3.2	hypothetical protein
BWI76_RS18780		+1.4	1.9	D-alanyl-D-alanine carboxypeptidase
BWI76_RS19525		+1.4	1.0	D-alanyl-D-alanine endopeptidase
BWI76_RS09155		+1.3	1.6	undecaprenyl-diphosphatase
BWI76_RS13550		+1.3	1.7	polar amino acid ABC transporter inner membrane subunit
BWI76_RS02180		+1.3	1.1	hypothetical protein
BWI76_RS24540		+1.3	1.5	hypothetical protein
BWI76_RS18345		+1.3	4.0	transcriptional regulator SdiA
BWI76_RS01540		+1.3	2.8	thiamine biosynthesis protein ThiS
BWI76_RS01195		+1.3	1.8	membrane protein
BWI76_RS10825		+1.3	1.4	deferrochelatase/peroxidase EfeB
BWI76_RS19465		+1.3	1.4	hypothetical protein
BWI76_RS07060		+1.3	1.4	carbamate kinase
BWI76_RS06795		+1.3	1.0	hypothetical protein
BWI76_RS14645		+1.3	1.4	sensor histidine kinase
BWI76_RS24055		+1.3	1.1	MFS transporter
BWI76_RS03165		+1.3	2.0	phage polarity suppression protein
BWI76_RS11610		+1.3	2.4	glutamate dehydrogenase
BWI76_RS17930		+1.3	1.8	cold-shock protein CspC
BWI76_RS21710		+1.2	1.1	nitroreductase
BWI76_RS08800		+1.2	1.2	phosphoethanolamine transferase EptA
BWI76_RS09630		+1.2	0.7	leucyl/phenylalanyl-tRNA--protein transferase
BWI76_RS09160		+1.2	1.6	multidrug transporter MdfA
BWI76_RS15135		+1.2	1.5	hypothetical protein
BWI76_RS21930		+1.2	1.7	LysR family transcriptional regulator
BWI76_RS01905		+1.2	1.9	envelope stress response protein PspG
BWI76_RS20700		+1.2	1.3	sulfate/thiosulfate transporter subunit
BWI76_RS06540		+1.2	3.3	DNA-binding protein HU-beta
BWI76_RS01560		+1.2	2.9	Rsd/AlgQ family anti-sigma factor
BWI76_RS23905		+1.2	1.1	Z-ring-associated protein
BWI76_RS16450		+1.2	1.5	fusaric acid resistance protein
BWI76_RS14045		+1.2	4.7	oxidoreductase
BWI76_RS20720		+1.2	1.5	hypothetical protein
BWI76_RS08510		+1.2	1.2	cyd operon protein YbgE
BWI76_RS27425		+1.2	1.9	DNA-directed RNA polymerase subunit omega
BWI76_RS26470		+1.2	1.0	4-deoxy-4-formamido-L-arabinose- phosphoundecaprenol deformylase
BWI76_RS07470		+1.1	0.9	MBL fold metallo-hydrolase
BWI76_RS24010		+1.1	1.2	hypothetical protein
BWI76_RS05785		+1.1	1.8	CI repressor
BWI76_RS20295		+1.1	0.8	dihydroneopterin triphosphate 2'-epimerase
BWI76_RS10905		+1.1	1.7	hypothetical protein
BWI76_RS18240		+1.1	1.7	L-arabinose ABC transporter permease AraH
BWI76_RS07820		+1.1	0.9	S-methyl-5-thioribose kinase
BWI76_RS17785		+1.1	1.4	septum site-determining protein MinD
BWI76_RS18335		+1.1	1.6	DNA-binding response regulator
BWI76_RS07560		+1.1	1.7	aminotransferase regulator domain-containing protein
BWI76_RS01360		+1.1	1.6	acetyl-CoA C-acyltransferase FadA
BWI76_RS01585		+1.1	4.8	transcriptional regulator
BWI76_RS06950		+1.1	1.2	ABC transporter ATP-binding protein
BWI76_RS01885		+1.1	1.4	CsbD family protein
BWI76_RS22265		+1.1	4.3	NADH:flavorubredoxin oxidoreductase
BWI76_RS22740		+1.1	1.2	type I-E CRISPR-associated protein Cse2/CasB
BWI76_RS21275		+1.1	0.9	lytic transglycosylase F
BWI76_RS01535		+1.1	1.9	thiazole synthase
BWI76_RS06835		+1.1	1.2	molecular chaperone HtpG
BWI76_RS11820		+1.1	1.4	short-chain dehydrogenase/reductase SDR
BWI76_RS15940		+1.1	1.3	DMSO reductase maturation protein DsmD
BWI76_RS20795		+1.1	1.1	ethanolamine catabolic microcompartment shell protein EutN
BWI76_RS03190		+1.1	1.7	hypothetical protein
BWI76_RS05880		+1.1	0.9	AraC-type DNA-binding domain-containing protein
BWI76_RS19355		+1.1	4.1	LacI family transcriptional regulator
BWI76_RS22135		+1.1	1.0	transport-associated protein
BWI76_RS12975		+1.1	1.3	type I glyceraldehyde-3-phosphate dehydrogenase
BWI76_RS25160		+1.1	1.7	transcription elongation factor GreA
BWI76_RS20635		+1.1	0.8	sulfate transporter CysZ
BWI76_RS00710		+1.0	1.6	6-N-hydroxylaminopurine resistance protein
BWI76_RS01530		+1.0	1.3	2-iminoacetate synthase ThiH
BWI76_RS21380		+1.0	1.3	uracil-DNA glycosylase
BWI76_RS18385		+1.0	1.1	hypothetical protein
BWI76_RS12715		+1.0	1.6	universal stress protein F
BWI76_RS18575		+1.0	2.7	transcriptional regulator
BWI76_RS26595		+1.0	1.5	FAD-dependent oxidoreductase
BWI76_RS02220		+1.0	2.5	carbon-phosphorus lyase subunit PhnH
BWI76_RS10300		+1.0	1.3	MFS transporter
BWI76_RS10705		+1.0	0.9	Gamma-glutamyl-gamma-aminobutyrate hydrolase (EC 3.5.1.94) (from data)
BWI76_RS11300		+1.0	1.3	cupin
BWI76_RS22290		+1.0	1.6	PTS cellobiose/arbutin/salicin transporter subunit IIBC
BWI76_RS15715		+1.0	2.4	putative ninG protein
BWI76_RS24335		+1.0	1.8	two-component system response regulator QseB
BWI76_RS09050		+1.0	2.5	MFS transporter
BWI76_RS21020		+1.0	2.3	MFS transporter
BWI76_RS06060		+1.0	1.7	DNA-binding response regulator
BWI76_RS14105		+1.0	3.3	NADH:ubiquinone reductase (Na(+)-transporting) subunit E
BWI76_RS21740		+1.0	1.6	transcriptional regulator, LysR family protein
BWI76_RS07430		+1.0	1.6	NUDIX hydrolase
BWI76_RS10560		+1.0	1.2	gamma-glutamyltransferase
BWI76_RS00115		+1.0	1.2	6-phosphogluconate phosphatase
BWI76_RS13850		+1.0	0.9	N-acetyltransferase
BWI76_RS09290		+1.0	2.7	phage Tail Protein X
BWI76_RS14215		+1.0	1.2	hypothetical protein
BWI76_RS18610		+1.0	1.4	transcriptional regulator LysR
BWI76_RS14030		+1.0	2.5	ABC transporter permease
BWI76_RS27650		+1.0	1.6	EamA family transporter
BWI76_RS23965		+0.9	1.2	arginine exporter protein
BWI76_RS16445		+0.9	1.0	auxiliary transporter membrane fusion protein
BWI76_RS20010		+0.9	1.9	catalase
BWI76_RS06410		+0.9	3.4	YajQ family cyclic di-GMP-binding protein
BWI76_RS11410		+0.9	1.4	leucine efflux protein
BWI76_RS14970		+0.9	1.5	pyrroline-5-carboxylate reductase
BWI76_RS07230		+0.9	2.1	transcriptional regulator
BWI76_RS25040		+0.9	1.1	hypothetical protein
BWI76_RS07475		+0.9	1.0	DNA-binding transcriptional regulator RamA
BWI76_RS05710		+0.9	1.0	TetR family transcriptional regulator
BWI76_RS22815		+0.9	1.9	hybrid sensor histidine kinase/response regulator
BWI76_RS11425		+0.9	1.4	GAF domain/diguanylate cyclase
BWI76_RS20090		+0.9	1.3	ribonuclease Z
BWI76_RS14200		+0.9	1.5	protocatechuate 4,5-dioxygenase
BWI76_RS21345		+0.9	1.2	sigma-E factor regulatory protein RseB
BWI76_RS00440		+0.9	1.4	CopG family transcriptional regulator
BWI76_RS08610		+0.9	0.9	galactose-1-epimerase
BWI76_RS24925		+0.9	1.8	DnaA initiator-associating protein DiaA
BWI76_RS22645		+0.9	3.7	RNA polymerase sigma factor RpoS
BWI76_RS25060		+0.9	2.0	lipoprotein NlpI
BWI76_RS20360		+0.9	0.9	cell division protein DedD
BWI76_RS08880		+0.9	1.4	secretion protein HlyD
BWI76_RS11515		+0.9	1.1	peptide-methionine (R)-S-oxide reductase
BWI76_RS05645		+0.9	4.0	fructuronate reductase
BWI76_RS12680		+0.9	1.2	oxidoreductase
BWI76_RS23325		+0.9	1.3	hypothetical protein
BWI76_RS19195		+0.9	2.1	nickel ABC transporter permease
BWI76_RS23085		+0.9	1.3	adenosylcobinamide-phosphate synthase
BWI76_RS07535		+0.9	1.4	dipeptide/oligopeptide/nickel ABC transporter ATP-binding protein
BWI76_RS24380		+0.8	1.5	esterase YqiA
BWI76_RS05555		+0.8	1.9	Crl family RNA polymerase assembly factor
BWI76_RS22130		+0.8	1.8	fructose-1-phosphate/6-phosphogluconate phosphatase
BWI76_RS05570		+0.8	0.8	hypothetical protein
BWI76_RS09820		+0.8	2.2	hypothetical protein
BWI76_RS25415		+0.8	1.2	GntR family transcriptional regulator
BWI76_RS11445		+0.8	1.3	hypothetical protein
BWI76_RS12520		+0.8	1.3	hypothetical protein
BWI76_RS24770		+0.8	1.1	hypothetical protein
BWI76_RS14095		+0.8	1.5	hypothetical protein
BWI76_RS01805		+0.8	1.0	UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
BWI76_RS03175		+0.8	1.0	hypothetical protein
BWI76_RS13740		+0.8	1.4	formate dehydrogenase-N subunit alpha
BWI76_RS20460		+0.8	0.9	acetyl-CoA C-acyltransferase FadI
BWI76_RS23400		+0.8	2.9	oligogalacturonide lyase
BWI76_RS23990		+0.8	1.2	DNA-binding protein
BWI76_RS24315		+0.8	1.2	cell division protein FtsP
BWI76_RS02750		+0.8	1.3	DNA-binding transcriptional regulator AraC
BWI76_RS17675		+0.8	0.7	hypothetical protein
BWI76_RS08595		+0.8	1.4	hypothetical protein
BWI76_RS06780		+0.8	0.7	MexE family multidrug efflux RND transporter periplasmic adaptor subunit
BWI76_RS11100		+0.8	1.6	beta-ketoacyl-[acyl-carrier-protein] synthase II

Specific Phenotypes

For 2 genes in this experiment