Experiment set31S7 for Bacteroides thetaiotaomicron VPI-5482
b-Glucan from Beet (1,2-b-Glucan); filtered 2.5 mg/ml carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + b-Glucan from Beet (1,2-b-Glucan) (2.5 mg/ml)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 3 genes in this experiment
For carbon source b-Glucan from Beet (1,2-b-Glucan) in Bacteroides thetaiotaomicron VPI-5482
For carbon source b-Glucan from Beet (1,2-b-Glucan) across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Beta-Glucoside Metabolism | 1 |
| Iron acquisition in Vibrio | 1 |
| Ton and Tol transport systems | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| neolinustatin bioactivation | 3 | 2 | 2 |
| linustatin bioactivation | 4 | 2 | 2 |
| linamarin degradation | 2 | 1 | 1 |
| lotaustralin degradation | 2 | 1 | 1 |
| cellulose degradation II (fungi) | 3 | 2 | 1 |
| coumarin biosynthesis (via 2-coumarate) | 5 | 2 | 1 |
| α-tomatine degradation | 6 | 1 | 1 |
| firefly bioluminescence | 14 | 2 | 1 |