Experiment set31S57 for Bacteroides thetaiotaomicron VPI-5482

Mannan from Saccharomyces cerevisiae; filtered 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + Mannan from Saccharomyces cerevisiae (2.5 mg/ml)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 8 genes in this experiment

For carbon source Mannan from Saccharomyces cerevisiae in Bacteroides thetaiotaomicron VPI-5482

For carbon source Mannan from Saccharomyces cerevisiae across organisms

SEED Subsystems

Subsystem	#Specific
Iron acquisition in Vibrio	1
Proline Synthesis	1
Ton and Tol transport systems	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Puromycin biosynthesis
• Arginine and proline metabolism
• Nucleotide sugars metabolism
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-proline biosynthesis III (from L-ornithine)	3	2	1
L-proline biosynthesis I (from L-glutamate)	4	4	1
L-arginine degradation VI (arginase 2 pathway)	4	3	1
L-arginine degradation XIII (reductive Stickland reaction)	5	2	1