Experiment set31S52 for Bacteroides thetaiotaomicron VPI-5482
Chondroitin sulfate A sodium salt from bovine trachea; filtered 2.5 mg/ml carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + Chondroitin sulfate A sodium salt from bovine trachea (2.5 mg/ml)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 9 genes in this experiment
For carbon source Chondroitin sulfate A sodium salt from bovine trachea across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
Pathway | #Steps | #Present | #Specific |
---|---|---|---|
2-deoxy-D-ribose degradation I | 3 | 2 | 1 |
2-deoxy-α-D-ribose 1-phosphate degradation | 3 | 1 | 1 |
choline-O-sulfate degradation | 3 | 1 | 1 |
4-deoxy-L-threo-hex-4-enopyranuronate degradation | 5 | 5 | 1 |
superpathway of pyrimidine deoxyribonucleosides degradation | 6 | 3 | 1 |
superpathway of purine deoxyribonucleosides degradation | 7 | 4 | 1 |
superpathway of microbial D-galacturonate and D-glucuronate degradation | 31 | 15 | 1 |