Experiment set31S51 for Bacteroides thetaiotaomicron VPI-5482

Chondroitin sulfate A sodium salt from bovine trachea; filtered 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + Chondroitin sulfate A sodium salt from bovine trachea (2.5 mg/ml)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 7 genes in this experiment

For carbon source Chondroitin sulfate A sodium salt from bovine trachea in Bacteroides thetaiotaomicron VPI-5482

For carbon source Chondroitin sulfate A sodium salt from bovine trachea across organisms

SEED Subsystems

Subsystem	#Specific
Choline Transport	1
Choline and Betaine Uptake and Betaine Biosynthesis	1
D-Galacturonate and D-Glucuronate Utilization	1
Iron acquisition in Vibrio	1
N-Acetyl-Galactosamine and Galactosamine Utilization	1
Ton and Tol transport systems	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose and glucuronate interconversions

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
choline-O-sulfate degradation	3	1	1
4-deoxy-L-threo-hex-4-enopyranuronate degradation	5	5	1
superpathway of microbial D-galacturonate and D-glucuronate degradation	31	15	1