Experiment set31S17 for Bacteroides thetaiotaomicron VPI-5482

Pectin from apple; autoclaved 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + Pectin from apple (2.5 mg/ml)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source Pectin from apple in Bacteroides thetaiotaomicron VPI-5482

For carbon source Pectin from apple across organisms

SEED Subsystems

Subsystem	#Specific
Alginate metabolism	1
D-Galacturonate and D-Glucuronate Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.