Experiment set31IT051 for Klebsiella michiganensis M5al
KLEB16 MOI 1
Group: phageMedia: LB_plus_SM_buffer + KLEB16 MOI 1 + Kan (50 ug/ml)
Culturing: Koxy_ML2a, 48 well microplate; Epoch2, Aerobic, at 30 (C), shaken=double orbital, continuous, 205cpm
By: Vivek on 12/8/21
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 4 genes in this experiment
For phage KLEB16 MOI 1 in Klebsiella michiganensis M5al
For phage KLEB16 MOI 1 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Peptidoglycan Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 5 | 2 |
| peptidoglycan recycling II | 10 | 7 | 1 |
| peptidoglycan recycling I | 14 | 14 | 1 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 1 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 1 |