Experiment set31IT049 for Klebsiella michiganensis M5al
KLEB16 MOI 10
Group: phageMedia: LB_plus_SM_buffer + KLEB16 MOI 10 + Kan (50 ug/ml)
Culturing: Koxy_ML2a, 48 well microplate; Epoch2, Aerobic, at 30 (C), shaken=double orbital, continuous, 205cpm
By: Vivek on 12/8/21
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 2 genes in this experiment
For phage KLEB16 MOI 10 in Klebsiella michiganensis M5al
For phage KLEB16 MOI 10 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| D-gluconate and ketogluconates metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fructose and mannose metabolism
- Ascorbate and aldarate metabolism
- C21-Steroid hormone metabolism
- Glycine, serine and threonine metabolism
- Bisphenol A degradation
- Nucleotide sugars metabolism
- Polyketide sugar unit biosynthesis
- Aminosugars metabolism
- Linoleic acid metabolism
- Tetrachloroethene degradation
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Retinol metabolism
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of type II polyketide products
- Biosynthesis of alkaloids derived from shikimate pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| methylglyoxal degradation III | 2 | 2 | 1 |
| L-ascorbate biosynthesis VIII (engineered pathway) | 7 | 4 | 2 |
| ketogluconate metabolism | 8 | 7 | 2 |
| superpathway of methylglyoxal degradation | 8 | 5 | 1 |
| detoxification of reactive carbonyls in chloroplasts | 10 | 1 | 1 |