Experiment set2S606 for Mycobacterium tuberculosis H37Rv
D,L-Malic Acid 10 mM carbon source
Group: carbon sourceMedia: Sautons minimal media with no carbon + D,L-Malic Acid (10 mM)
Culturing: MycoTube_ML10, tube, Aerobic, at 37 (C)
By: Kayla Dinshaw on 10/2/23
Media components: 0.8 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L Potassium phosphate monobasic, 0.2 g/L L-Asparagine, 0.05 g/L Ferric ammonium citrate, 0.02 g/L Magnesium sulfate, 0.0005 g/L Calcium chloride, 1e-05 g/L Zinc sulfate heptahydrate, 0.2 vol% Ethanol, 0.05 vol% Tyloxapol
Specific Phenotypes
For 1 genes in this experiment
For carbon source D,L-Malic Acid in Mycobacterium tuberculosis H37Rv
For carbon source D,L-Malic Acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Ketoisovalerate oxidoreductase | 1 |
| Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
- Propanoate metabolism
- Reductive carboxylate cycle (CO2 fixation)
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| acetate and ATP formation from acetyl-CoA III | 1 | 1 | 1 |
| acetate conversion to acetyl-CoA | 1 | 1 | 1 |
| superpathway of acetate utilization and formation | 3 | 3 | 1 |
| ethanol degradation IV | 3 | 3 | 1 |
| ethanol degradation II | 3 | 3 | 1 |
| ethanol degradation III | 3 | 2 | 1 |
| L-isoleucine biosynthesis V | 3 | 2 | 1 |
| chitin deacetylation | 4 | 3 | 1 |
| 2-methylcitrate cycle I | 5 | 4 | 1 |
| β-alanine biosynthesis II | 6 | 5 | 1 |
| L-isoleucine biosynthesis IV | 6 | 4 | 1 |
| 2-methylcitrate cycle II | 6 | 3 | 1 |
| superpathway of bitter acids biosynthesis | 18 | 3 | 3 |
| colupulone and cohumulone biosynthesis | 6 | 1 | 1 |
| lupulone and humulone biosynthesis | 6 | 1 | 1 |
| adlupulone and adhumulone biosynthesis | 6 | 1 | 1 |
| reductive glycine pathway of autotrophic CO2 fixation | 9 | 4 | 1 |
| cis-geranyl-CoA degradation | 9 | 1 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 9 | 1 |