Experiment set2S568 for Synechocystis sp000284455 PCC 6803

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high light; bubbling

Group: light_bubbling
Media: BG11C
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=air bubling
By: Luis on 6/30/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 40 genes in this experiment

SEED Subsystems

Subsystem #Specific
Coenzyme F420 synthesis 2
Cyanobacterial Circadian Clock 2
ZZ gjo need homes 2
Bacterial Cell Division 1
Bacterial hemoglobins 1
Cobalamin synthesis 1
Coenzyme B12 biosynthesis 1
Conserved gene cluster possibly involved in RNA metabolism 1
High affinity phosphate transporter and control of PHO regulon 1
Phosphate metabolism 1
Photosystem I 1
Photosystem II 1
Sialic Acid Metabolism 1
Synechocystis experimental 1
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis 1
Ubiquinone Biosynthesis 1
tRNA aminoacylation, Asp and Asn 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
menaquinol-4 biosynthesis I 1 1 1
menaquinol-9 biosynthesis 1 1 1
menaquinol-6 biosynthesis 1 1 1
menaquinol-8 biosynthesis 1 1 1
photosynthesis light reactions 4 4 2
menaquinol-10 biosynthesis 2 2 1
menaquinol-11 biosynthesis 2 2 1
menaquinol-7 biosynthesis 2 2 1
menaquinol-12 biosynthesis 2 2 1
pyrimidine nucleobases salvage II 2 2 1
menaquinol-13 biosynthesis 2 2 1
UDP-α-D-glucose biosynthesis 2 1 1
protective electron sinks in the thylakoid membrane (PSII to PTOX) 2 1 1
pyrimidine ribonucleosides salvage III 2 1 1
superpathway of photosynthetic hydrogen production 6 5 2
GDP-α-D-glucose biosynthesis 3 2 1
trehalose degradation V 3 2 1
cyclic electron flow 3 2 1
glycogen biosynthesis I (from ADP-D-Glucose) 4 4 1
starch degradation V 4 3 1
phylloquinol biosynthesis 4 3 1
sucrose degradation IV (sucrose phosphorylase) 4 3 1
starch degradation III 4 2 1
dTDP-β-L-rhamnose biosynthesis 5 5 1
glucosylglycerol biosynthesis 5 5 1
UDP-N-acetyl-D-glucosamine biosynthesis I 5 5 1
glucose and glucose-1-phosphate degradation 5 3 1
sucrose degradation II (sucrose synthase) 5 3 1
CDP-6-deoxy-D-gulose biosynthesis 5 3 1
D-galactose degradation I (Leloir pathway) 5 2 1
O-antigen building blocks biosynthesis (E. coli) 11 10 2
glycogen degradation II 6 4 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 3 1
sucrose biosynthesis II 8 7 1
glycogen degradation I 8 7 1
ubiquinol-8 biosynthesis (early decarboxylation) 8 4 1
ubiquinol-9 biosynthesis (early decarboxylation) 8 3 1
ubiquinol-9 biosynthesis (late decarboxylation) 8 2 1
glycogen biosynthesis III (from α-maltose 1-phosphate) 8 2 1
ubiquinol-6 biosynthesis (late decarboxylation) 8 1 1
ubiquinol-7 biosynthesis (late decarboxylation) 8 1 1
ubiquinol-10 biosynthesis (early decarboxylation) 8 1 1
ubiquinol-7 biosynthesis (early decarboxylation) 8 1 1
oxygenic photosynthesis 17 17 2
sucrose biosynthesis I (from photosynthesis) 9 8 1
chitin biosynthesis 9 5 1
ubiquinol-8 biosynthesis (late decarboxylation) 9 2 1
ubiquinol-10 biosynthesis (late decarboxylation) 9 1 1
ubiquinol-6 biosynthesis from 4-aminobenzoate (yeast) 9 1 1
superpathway of menaquinol-8 biosynthesis III 9 1 1
superpathway of menaquinol-10 biosynthesis 10 9 1
superpathway of menaquinol-11 biosynthesis 10 9 1
superpathway of menaquinol-9 biosynthesis 10 9 1
superpathway of menaquinol-7 biosynthesis 10 9 1
superpathway of menaquinol-12 biosynthesis 10 9 1
superpathway of menaquinol-8 biosynthesis I 10 9 1
superpathway of menaquinol-13 biosynthesis 10 9 1
superpathway of menaquinol-6 biosynthesis 10 9 1
starch biosynthesis 10 6 1
CMP-legionaminate biosynthesis I 10 2 1
superpathway of menaquinol-8 biosynthesis II 10 1 1
colanic acid building blocks biosynthesis 11 10 1
superpathway of ubiquinol-6 biosynthesis (late decarboxylation) 11 1 1
superpathway of ubiquinol-8 biosynthesis (early decarboxylation) 12 6 1
ethene biosynthesis V (engineered) 25 24 2
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 23 2
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) 13 11 1
1-butanol autotrophic biosynthesis (engineered) 27 22 2
peptidoglycan recycling I 14 9 1
superpathway of phylloquinol biosynthesis 15 14 1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) 15 14 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 13 2
streptomycin biosynthesis 18 2 1
superpathway of anaerobic sucrose degradation 19 14 1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis 19 6 1
tRNA charging 21 20 1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis 24 6 1
superpathway of chorismate metabolism 59 43 2
adenosylcobalamin biosynthesis II (aerobic) 33 28 1
adenosylcobalamin biosynthesis I (anaerobic) 36 31 1
colibactin biosynthesis 38 7 1