Experiment set2S567 for Synechocystis sp000284455 PCC 6803

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medium light; bubbling

Group: light_bubbling
Media: BG11C
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=air bubling
By: Luis on 6/30/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 23 genes in this experiment

SEED Subsystems

Subsystem #Specific
Protein chaperones 2
Bacterial hemoglobins 1
Cobalamin synthesis 1
Coenzyme B12 biosynthesis 1
DNA-replication 1
DNA repair, UvrABC system 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Heat shock dnaK gene cluster extended 1
Macromolecular synthesis operon 1
Peptidoglycan Biosynthesis 1
Proteolysis in bacteria, ATP-dependent 1
Sialic Acid Metabolism 1
Threonine and Homoserine Biosynthesis 1
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis 1
Ubiquinone Biosynthesis 1
tRNA aminoacylation, Asp and Asn 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-aspartate degradation I 1 1 1
L-aspartate biosynthesis 1 1 1
3-(4-hydroxyphenyl)pyruvate biosynthesis 1 1 1
malate/L-aspartate shuttle pathway 2 2 1
atromentin biosynthesis 2 1 1
L-tyrosine degradation II 2 1 1
L-tryptophan degradation IV (via indole-3-lactate) 2 1 1
L-glutamate degradation II 2 1 1
UDP-α-D-glucose biosynthesis 2 1 1
L-phenylalanine biosynthesis I 3 3 1
L-asparagine degradation III (mammalian) 3 2 1
trehalose degradation V 3 2 1
L-tyrosine biosynthesis I 3 2 1
GDP-α-D-glucose biosynthesis 3 2 1
L-tyrosine degradation IV (to 4-methylphenol) 3 1 1
sulfolactate degradation III 3 1 1
(R)-cysteate degradation 3 1 1
L-phenylalanine degradation II (anaerobic) 3 1 1
indole-3-acetate biosynthesis VI (bacteria) 3 1 1
glycogen biosynthesis I (from ADP-D-Glucose) 4 4 1
sucrose degradation IV (sucrose phosphorylase) 4 3 1
starch degradation V 4 3 1
starch degradation III 4 2 1
superpathway of L-aspartate and L-asparagine biosynthesis 4 2 1
L-phenylalanine degradation III 4 2 1
L-tyrosine degradation III 4 2 1
5,5'-dehydrodivanillate degradation 4 1 1
2-amino-3-carboxymuconate semialdehyde degradation to 2-hydroxypentadienoate 4 1 1
L-tryptophan degradation VIII (to tryptophol) 4 1 1
glucosylglycerol biosynthesis 5 5 1
dTDP-β-L-rhamnose biosynthesis 5 5 1
UDP-N-acetyl-D-glucosamine biosynthesis I 5 5 1
CDP-6-deoxy-D-gulose biosynthesis 5 3 1
glucose and glucose-1-phosphate degradation 5 3 1
superpathway of plastoquinol biosynthesis 5 3 1
trans-4-hydroxy-L-proline degradation I 5 3 1
sucrose degradation II (sucrose synthase) 5 3 1
D-galactose degradation I (Leloir pathway) 5 2 1
L-tyrosine degradation I 5 2 1
protein O-mannosylation II (mammals, core M1 and core M2) 5 1 1
L-tyrosine degradation V (reductive Stickland reaction) 5 1 1
L-tryptophan degradation XIII (reductive Stickland reaction) 5 1 1
protein O-mannosylation III (mammals, core M3) 5 1 1
4-hydroxybenzoate biosynthesis I (eukaryotes) 5 1 1
2-amino-3-carboxymuconate semialdehyde degradation to glutaryl-CoA 5 1 1
L-phenylalanine degradation VI (reductive Stickland reaction) 5 1 1
O-antigen building blocks biosynthesis (E. coli) 11 10 2
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 6 2
TCA cycle VIII (Chlamydia) 6 6 1
superpathway of L-threonine biosynthesis 6 6 1
glycogen degradation II 6 4 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 3 1
peptidoglycan maturation (meso-diaminopimelate containing) 12 4 2
superpathway of sulfolactate degradation 6 2 1
coenzyme M biosynthesis II 6 1 1
anaerobic energy metabolism (invertebrates, cytosol) 7 4 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 6 2
2-nitrobenzoate degradation I 7 1 1
glycogen degradation I 8 7 1
sucrose biosynthesis II 8 7 1
ubiquinol-8 biosynthesis (early decarboxylation) 8 4 1
glycogen biosynthesis III (from α-maltose 1-phosphate) 8 2 1
protein O-mannosylation I (yeast) 8 1 1
superpathway of aromatic amino acid biosynthesis 18 17 2
sucrose biosynthesis I (from photosynthesis) 9 8 1
chitin biosynthesis 9 5 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 5 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 3 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 9 1
starch biosynthesis 10 6 1
CMP-legionaminate biosynthesis I 10 2 1
rosmarinic acid biosynthesis I 10 1 1
colanic acid building blocks biosynthesis 11 10 1
(S)-reticuline biosynthesis I 11 1 1
protein N-glycosylation (Haloferax volcanii) 11 1 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 8 1
superpathway of ubiquinol-8 biosynthesis (early decarboxylation) 12 6 1
indole-3-acetate biosynthesis II 12 3 1
L-tryptophan degradation XII (Geobacillus) 12 2 1
L-tryptophan degradation IX 12 2 1
superpathway of L-isoleucine biosynthesis I 13 13 1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) 13 11 1
peptidoglycan recycling I 14 9 1
superpathway of rosmarinic acid biosynthesis 14 1 1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) 15 14 1
L-tryptophan degradation III (eukaryotic) 15 3 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 13 2
peptidoglycan biosynthesis II (staphylococci) 17 12 1
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 1
superpathway of anaerobic energy metabolism (invertebrates) 17 9 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 12 1
streptomycin biosynthesis 18 2 1
superpathway of anaerobic sucrose degradation 19 14 1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis 19 6 1
protein N-glycosylation initial phase (eukaryotic) 19 1 1
superpathway of chorismate metabolism 59 43 3
tRNA charging 21 20 1
L-tryptophan degradation XI (mammalian, via kynurenine) 23 5 1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis 24 6 1
aspartate superpathway 25 19 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 1 1
adenosylcobalamin biosynthesis II (aerobic) 33 28 1
adenosylcobalamin biosynthesis I (anaerobic) 36 31 1
colibactin biosynthesis 38 7 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 20 1