Experiment set2S559 for Synechocystis sp000284455 PCC 6803

Guanidine Hydrochloride nitrogen source

Group: nitrogen source
Media: BG11C_TES + Guanidine Hydrochloride (5 mM)
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=100 rpm
By: Luis on 6/30/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, 20 mM TES, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 21 genes in this experiment

For nitrogen source Guanidine Hydrochloride in Synechocystis sp000284455 PCC 6803

For nitrogen source Guanidine Hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter branched-chain amino acid (TC 3.A.1.4.1)	2
Coenzyme F420 synthesis	2
NiFe hydrogenase maturation	2
Urea decomposition	2
Ammonia assimilation	1
Arginine and Ornithine Degradation	1
Bacterial hemoglobins	1
Cytochrome B6-F complex	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Glycogen metabolism	1
Polyamine Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups
• Purine metabolism
• Glutamate metabolism
• D-Glutamine and D-glutamate metabolism
• Starch and sucrose metabolism
• Atrazine degradation
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamine degradation I	1	1	1
urea degradation II	1	1	1
L-glutamate biosynthesis I	2	2	1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	2	1
putrescine biosynthesis I	2	2	1
ammonia assimilation cycle III	3	3	1
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
glycogen biosynthesis I (from ADP-D-Glucose)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	4	1
superpathway of putrescine biosynthesis	4	2	1
L-glutamate and L-glutamine biosynthesis	7	6	1
L-citrulline biosynthesis	8	5	1
superpathway of polyamine biosynthesis I	8	3	1
superpathway of allantoin degradation in plants	8	1	1
starch biosynthesis	10	6	1
NiFe(CO)(CN)2 cofactor biosynthesis	10	3	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	5	1
superpathway of L-citrulline metabolism	12	7	1
superpathway of L-arginine and L-ornithine degradation	13	6	1
superpathway of arginine and polyamine biosynthesis	17	11	1