Experiment set2S558 for Synechocystis sp000284455 PCC 6803

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Ammonium chloride nitrogen source

Group: nitrogen source
Media: BG11C_TES + Ammonium chloride (5 mM)
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=100 rpm
By: Luis on 6/30/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, 20 mM TES, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 1 genes in this experiment

For nitrogen source Ammonium chloride in Synechocystis sp000284455 PCC 6803

For nitrogen source Ammonium chloride across organisms

SEED Subsystems

Subsystem #Specific
CO2 uptake, carboxysome 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
CO2 fixation into oxaloacetate (anaplerotic) 2 2 1
cyanate degradation 3 3 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 6 1
3-hydroxypropanoate cycle 13 4 1
glyoxylate assimilation 13 3 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 6 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 5 1
superpathway of the 3-hydroxypropanoate cycle 18 4 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 20 1