Experiment set2S553 for Synechocystis sp000284455 PCC 6803

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high light; bubbling

Group: light_bubbling
Media: BG11C
Culturing: Synechocystis_PCC6803_ML3, vertical flask, 5% CO2 enriched air bubling, growth chamber, continous light, at 30 (C), shaken=100 rpm
By: Luis on 6/30/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 13 genes in this experiment

SEED Subsystems

Subsystem #Specific
Coenzyme F420 synthesis 2
Cobalamin synthesis 1
Coenzyme B12 biosynthesis 1
Cyanobacterial Circadian Clock 1
Sialic Acid Metabolism 1
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis 1
tRNA aminoacylation, Asp and Asn 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
menaquinol-6 biosynthesis 1 1 1
menaquinol-9 biosynthesis 1 1 1
menaquinol-4 biosynthesis I 1 1 1
menaquinol-8 biosynthesis 1 1 1
menaquinol-10 biosynthesis 2 2 1
menaquinol-7 biosynthesis 2 2 1
menaquinol-11 biosynthesis 2 2 1
menaquinol-12 biosynthesis 2 2 1
menaquinol-13 biosynthesis 2 2 1
UDP-α-D-glucose biosynthesis 2 1 1
trehalose degradation V 3 2 1
GDP-α-D-glucose biosynthesis 3 2 1
glycogen biosynthesis I (from ADP-D-Glucose) 4 4 1
phylloquinol biosynthesis 4 3 1
starch degradation V 4 3 1
sucrose degradation IV (sucrose phosphorylase) 4 3 1
starch degradation III 4 2 1
glucosylglycerol biosynthesis 5 5 1
dTDP-β-L-rhamnose biosynthesis 5 5 1
UDP-N-acetyl-D-glucosamine biosynthesis I 5 5 1
CDP-6-deoxy-D-gulose biosynthesis 5 3 1
sucrose degradation II (sucrose synthase) 5 3 1
glucose and glucose-1-phosphate degradation 5 3 1
D-galactose degradation I (Leloir pathway) 5 2 1
O-antigen building blocks biosynthesis (E. coli) 11 10 2
glycogen degradation II 6 4 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 3 1
sucrose biosynthesis II 8 7 1
glycogen degradation I 8 7 1
glycogen biosynthesis III (from α-maltose 1-phosphate) 8 2 1
sucrose biosynthesis I (from photosynthesis) 9 8 1
chitin biosynthesis 9 5 1
superpathway of menaquinol-8 biosynthesis III 9 1 1
superpathway of menaquinol-11 biosynthesis 10 9 1
superpathway of menaquinol-7 biosynthesis 10 9 1
superpathway of menaquinol-6 biosynthesis 10 9 1
superpathway of menaquinol-8 biosynthesis I 10 9 1
superpathway of menaquinol-12 biosynthesis 10 9 1
superpathway of menaquinol-13 biosynthesis 10 9 1
superpathway of menaquinol-10 biosynthesis 10 9 1
superpathway of menaquinol-9 biosynthesis 10 9 1
starch biosynthesis 10 6 1
CMP-legionaminate biosynthesis I 10 2 1
superpathway of menaquinol-8 biosynthesis II 10 1 1
colanic acid building blocks biosynthesis 11 10 1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) 13 11 1
peptidoglycan recycling I 14 9 1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) 15 14 1
superpathway of phylloquinol biosynthesis 15 14 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 13 2
streptomycin biosynthesis 18 2 1
superpathway of anaerobic sucrose degradation 19 14 1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis 19 6 1
tRNA charging 21 20 1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis 24 6 1
adenosylcobalamin biosynthesis II (aerobic) 33 28 1
adenosylcobalamin biosynthesis I (anaerobic) 36 31 1
colibactin biosynthesis 38 7 1
superpathway of chorismate metabolism 59 43 1