Experiment set2S540 for Synechocystis sp000284455 PCC 6803

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Nickel (II) sulfate hexahydrate 3 uM

Group: stress
Media: BG11C + Nickel (II) sulfate hexahydrate (3 uM)
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=100 rpm
By: Luis on 6/30/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 12 genes in this experiment

For stress Nickel (II) sulfate hexahydrate in Synechocystis sp000284455 PCC 6803

For stress Nickel (II) sulfate hexahydrate across organisms

SEED Subsystems

Subsystem #Specific
Peptidoglycan Biosynthesis 1
Synechocystis experimental 1
Ubiquinone Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
peptidoglycan maturation (meso-diaminopimelate containing) 12 4 2
ubiquinol-8 biosynthesis (early decarboxylation) 8 4 1
ubiquinol-9 biosynthesis (early decarboxylation) 8 3 1
ubiquinol-9 biosynthesis (late decarboxylation) 8 2 1
ubiquinol-7 biosynthesis (early decarboxylation) 8 1 1
ubiquinol-10 biosynthesis (early decarboxylation) 8 1 1
ubiquinol-6 biosynthesis (late decarboxylation) 8 1 1
ubiquinol-7 biosynthesis (late decarboxylation) 8 1 1
ubiquinol-8 biosynthesis (late decarboxylation) 9 2 1
ubiquinol-10 biosynthesis (late decarboxylation) 9 1 1
ubiquinol-6 biosynthesis from 4-aminobenzoate (yeast) 9 1 1
superpathway of ubiquinol-6 biosynthesis (late decarboxylation) 11 1 1
superpathway of ubiquinol-8 biosynthesis (early decarboxylation) 12 6 1
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 1
peptidoglycan biosynthesis II (staphylococci) 17 12 1
superpathway of chorismate metabolism 59 43 1