Experiment set2S538 for Synechocystis sp000284455 PCC 6803
Copper (II) sulfate pentahydrate 5 uM
Group: stressMedia: BG11C + Copper (II) sulfate pentahydrate (5 uM)
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=100 rpm
By: Luis on 6/30/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)
Specific Phenotypes
For 12 genes in this experiment
For stress Copper (II) sulfate pentahydrate in Synechocystis sp000284455 PCC 6803
For stress Copper (II) sulfate pentahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Restriction-Modification System | 1 |
| Soluble cytochromes and functionally related electron carriers | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- N-Glycan biosynthesis
- Fructose and mannose metabolism
- O-Glycan biosynthesis
- High-mannose type N-glycan biosynthesis
- O-Mannosyl glycan biosynthesis
- Keratan sulfate biosynthesis
- Lipopolysaccharide biosynthesis
- Peptidoglycan biosynthesis
- Glycerolipid metabolism
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- Sphingolipid metabolism
- Glycosphingolipid biosynthesis - lacto and neolacto series
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - ganglio series
- Carotenoid biosynthesis - General
- Zeatin biosynthesis
- Flavonoid biosynthesis
- Anthocyanin biosynthesis
- Flavone and flavonol biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| protein O-mannosylation II (mammals, core M1 and core M2) | 5 | 1 | 1 |
| protein O-mannosylation III (mammals, core M3) | 5 | 1 | 1 |
| protein O-mannosylation I (yeast) | 8 | 1 | 1 |
| protein N-glycosylation (Haloferax volcanii) | 11 | 1 | 1 |
| protein N-glycosylation initial phase (eukaryotic) | 19 | 1 | 1 |