Experiment set2S534 for Synechocystis sp000284455 PCC 6803

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Ethanol 2 vol%

Group: stress
Media: BG11C + Ethanol (2 vol%)
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=100 rpm
By: Luis on 6/9/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 16 genes in this experiment

For stress Ethanol in Synechocystis sp000284455 PCC 6803

For stress Ethanol across organisms

SEED Subsystems

Subsystem #Specific
Cyanobacterial Circadian Clock 3
Bacterial hemoglobins 1
Cobalamin synthesis 1
Coenzyme B12 biosynthesis 1
Oxidative stress 1
Oxygen and light sensor PpaA-PpsR 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
Salmonella enterica serotype O:3,10 O antigen biosynthesis 5 1 1
Salmonella enterica serotype O:9,46 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:4 O antigen biosynthesis (group B1) 6 1 1
Porphyromonas gingivalis O-LPS antigen biosynthesis 6 1 1
Salmonella enterica serotype O:2 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:9 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:8 O antigen biosynthesis 8 1 1
Salmonella enterica serotype O:9,46,27 O antigen biosynthesis 9 1 1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) 13 11 1
succinoglycan biosynthesis 14 1 1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) 15 14 1
adenosylcobalamin biosynthesis II (aerobic) 33 28 1
adenosylcobalamin biosynthesis I (anaerobic) 36 31 1