Experiment set2S533 for Synechocystis sp000284455 PCC 6803

D-Sorbitol 500 mM

Group: stress
Media: BG11C + D-Sorbitol (500 mM)
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=100 rpm
By: Luis on 6/9/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 29 genes in this experiment

For stress D-Sorbitol in Synechocystis sp000284455 PCC 6803

For stress D-Sorbitol across organisms

SEED Subsystems

Subsystem	#Specific
Urea decomposition	4
Di-Inositol-Phosphate biosynthesis	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Heat shock dnaK gene cluster extended	1
Queuosine-Archaeosine Biosynthesis	1
Respiratory dehydrogenases 1	1
Transport of Manganese	1
Trehalose Biosynthesis	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Carotenoid biosynthesis - General
• Pentose and glucuronate interconversions
• Biosynthesis of steroids
• Puromycin biosynthesis
• Lysine degradation
• Starch and sucrose metabolism
• N-Glycan biosynthesis
• Nucleotide sugars metabolism
• Streptomycin biosynthesis
• Lipopolysaccharide biosynthesis
• Peptidoglycan biosynthesis
• Inositol phosphate metabolism
• Glycerophospholipid metabolism
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Biotin metabolism
• Porphyrin and chlorophyll metabolism
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Phosphatidylinositol signaling system

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycerol-3-phosphate shuttle	2	2	1
nitrate reduction IX (dissimilatory)	2	1	1
trehalose biosynthesis I	2	1	1
glycerol-3-phosphate to fumarate electron transfer	2	1	1
myo-inositol biosynthesis	2	1	1
glycerophosphodiester degradation	2	1	1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer	2	1	1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer	2	1	1
glycerol-3-phosphate to hydrogen peroxide electron transport	2	1	1
glycerol degradation I	3	2	1
sn-glycerol 3-phosphate anaerobic respiration	3	1	1
D-myo-inositol (1,4,5)-trisphosphate degradation	3	1	1
queuosine biosynthesis I (de novo)	4	3	1
glycerol and glycerophosphodiester degradation	4	2	1
glucosylglycerol biosynthesis	5	5	1
queuosine biosynthesis III (queuosine salvage)	5	2	1
protein O-mannosylation II (mammals, core M1 and core M2)	5	1	1
protein O-mannosylation III (mammals, core M3)	5	1	1
superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism	6	1	1
phytate degradation I	14	2	2
protein O-mannosylation I (yeast)	8	1	1
protein N-glycosylation (Haloferax volcanii)	11	1	1
protein N-glycosylation initial phase (eukaryotic)	19	1	1