Experiment set2S520 for Synechocystis sp000284455 PCC 6803

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medium light; bubbling

Group: light_bubbling
Media: BG11C
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=air bubling
By: Luis on 5/26/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 22 genes in this experiment

SEED Subsystems

Subsystem #Specific
DNA-replication 1
Glycogen metabolism 1
Macromolecular synthesis operon 1
Methionine Biosynthesis 1
Methionine Degradation 1
Queuosine-Archaeosine Biosynthesis 1
Terminal cytochrome C oxidases 1
Trehalose Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
menaquinol-9 biosynthesis 1 1 1
menaquinol-8 biosynthesis 1 1 1
menaquinol-6 biosynthesis 1 1 1
menaquinol-4 biosynthesis I 1 1 1
menaquinol-12 biosynthesis 2 2 1
menaquinol-13 biosynthesis 2 2 1
menaquinol-7 biosynthesis 2 2 1
menaquinol-11 biosynthesis 2 2 1
menaquinol-10 biosynthesis 2 2 1
arsenite to oxygen electron transfer 2 1 1
succinate to cytochrome c oxidase via plastocyanin (thylakoid membrane) 3 2 1
succinate to chytochrome c oxidase via cytochrome c6 (thylakoid membrane) 3 1 1
arsenite to oxygen electron transfer (via azurin) 3 1 1
aerobic respiration I (cytochrome c) 4 3 1
queuosine biosynthesis I (de novo) 4 3 1
NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane) 4 3 1
aerobic respiration II (cytochrome c) (yeast) 4 2 1
(2R)-methoxymalonyl-[acp] biosynthesis 4 1 1
creatinine degradation I 4 1 1
queuosine biosynthesis III (queuosine salvage) 5 2 1
Salmonella enterica serotype O:3,10 O antigen biosynthesis 5 1 1
Fe(II) oxidation 6 2 1
Porphyromonas gingivalis O-LPS antigen biosynthesis 6 1 1
Salmonella enterica serotype O:2 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:9 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:9,46 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:4 O antigen biosynthesis (group B1) 6 1 1
Salmonella enterica serotype O:8 O antigen biosynthesis 8 1 1
Salmonella enterica serotype O:9,46,27 O antigen biosynthesis 9 1 1
superpathway of menaquinol-8 biosynthesis III 9 1 1
superpathway of menaquinol-8 biosynthesis I 10 9 1
superpathway of menaquinol-13 biosynthesis 10 9 1
superpathway of menaquinol-9 biosynthesis 10 9 1
superpathway of menaquinol-12 biosynthesis 10 9 1
superpathway of menaquinol-6 biosynthesis 10 9 1
superpathway of menaquinol-7 biosynthesis 10 9 1
superpathway of menaquinol-10 biosynthesis 10 9 1
superpathway of menaquinol-11 biosynthesis 10 9 1
peptidoglycan recycling II 10 3 1
superpathway of menaquinol-8 biosynthesis II 10 1 1
peptidoglycan recycling I 14 9 1
succinoglycan biosynthesis 14 1 1
superpathway of chorismate metabolism 59 43 1