Experiment set2S435 for Phocaeicola vulgatus CL09T03C04

Galactose 1-phosphate dipotassium salt pentahydrate carbon source

Group: carbon source
Media: Varel_Bryant_medium + Galactose 1-phosphate dipotassium salt pentahydrate (20 mM)
Culturing: Bvulgatus_CL09T03C04_ML5, 24-well microplate; 1.2 mL volume, Anaerobic, at 34 (C), shaken=0 rpm
By: Surya Tripathi on 3-Jul-23
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 10 genes in this experiment

For carbon source Galactose 1-phosphate dipotassium salt pentahydrate in Phocaeicola vulgatus CL09T03C04

For carbon source Galactose 1-phosphate dipotassium salt pentahydrate across organisms

SEED Subsystems

Subsystem	#Specific
L-fucose utilization	1
L-fucose utilization temp	1
Phosphate metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism
• gamma-Hexachlorocyclohexane degradation
• Folate biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
diethylphosphate degradation	2	1	1
pyruvate-derived opine biosynthesis I (bacteria)	3	1	1
pyruvate-derived opine biosynthesis II (marine invertebrates)	5	1	1