Experiment set2S419 for Phocaeicola vulgatus CL09T03C04

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D-Mannose carbon source

Group: carbon source
Media: Varel_Bryant_medium + D-Mannose (20 mM)
Culturing: Bvulgatus_CL09T03C04_ML5, 24-well microplate; 1.2 mL volume, Anaerobic, at 34 (C), shaken=0 rpm
By: Surya Tripathi on 3-Jul-23
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 6 genes in this experiment

For carbon source D-Mannose in Phocaeicola vulgatus CL09T03C04

For carbon source D-Mannose across organisms

SEED Subsystems

Subsystem #Specific
Campylobacter Iron Metabolism 1
Carboxysome 1
Entner-Doudoroff Pathway 1
Glycolysis and Gluconeogenesis 1
Pentose phosphate pathway 1
Respiratory dehydrogenases 1 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
NADH to cytochrome bo oxidase electron transfer I 2 1 1
NADH to cytochrome bd oxidase electron transfer I 2 1 1
pentose phosphate pathway (oxidative branch) I 3 3 1
aerobic respiration III (alternative oxidase pathway) 3 2 1
aerobic respiration I (cytochrome c) 4 2 1
formaldehyde oxidation I 6 3 1
NAD(P)/NADPH interconversion 6 2 1
Fe(II) oxidation 6 1 1
pentose phosphate pathway 8 8 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 14 2
Entner-Doudoroff pathway I 9 8 1
sucrose biosynthesis I (from photosynthesis) 9 6 1
glycolysis II (from fructose 6-phosphate) 11 10 1
gluconeogenesis III 12 10 1
formaldehyde assimilation III (dihydroxyacetone cycle) 12 10 1
gluconeogenesis I 13 11 1
glycolysis I (from glucose 6-phosphate) 13 11 1
Calvin-Benson-Bassham cycle 13 9 1
superpathway of glucose and xylose degradation 17 16 1
oxygenic photosynthesis 17 9 1
heterolactic fermentation 18 14 1
superpathway of hexitol degradation (bacteria) 18 12 1
superpathway of anaerobic sucrose degradation 19 14 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of N-acetylneuraminate degradation 22 17 1
ethene biosynthesis V (engineered) 25 15 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 19 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 13 1
1-butanol autotrophic biosynthesis (engineered) 27 14 1